Cryogenic electron tomography and elemental analysis of mitochondrial granules in human retinal ganglion cells [0.03%]
人视网膜神经节细胞线粒体颗粒的低温电子层析成像和元素分析
Gong-Her Wu,Cathy Hou,Andrew Thron et al.
Gong-Her Wu et al.
Combining three-dimensional (3D) visualization with elemental analysis of vitrified cells can provide crucial insights into subcellular structures and elemental compositions in their native environments. We present a coordinated approach us...
Crystal structures of PAK2 reveal new insights into its autoinhibitory mechanism [0.03%]
PAK2的晶体结构揭示了其新的自身抑制机制的见解
Hui-Fang Hu,Zhipu Luo,Yikan Zhang et al.
Hui-Fang Hu et al.
Type I p21-activated kinases (PAK1/2/3) exist in an auto-inhibited form and are stimulated by small G-protein binding and auto-phosphorylation. Previous structural and biochemical studies suggested that PAK1 is a dimer in crystals, and prob...
Structural basis of pseudoGTPase-mediated protein-protein interactions [0.03%]
伪GTP酶介导的蛋白质-蛋白质相互作用的结构基础
Bing Wang,Rui Yang,Chun Wan et al.
Bing Wang et al.
GTPases regulate cellular processes through conformational changes triggered by guanine nucleotide binding. Recently, pseudoGTPases, the catalytically inactive counterparts of GTPases, have been identified across species from bacteria to hu...
Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV [0.03%]
在100 kV下利用单颗粒冷冻电镜进行低于3 Å的蛋白质结构测定
Dimple Karia,Adrian F Koh,Wen Yang et al.
Dimple Karia et al.
Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report sub-3 Å resolution structures using the 100 keV Tundra cryo-TEM, equipped with the Falcon ...
Antibody-like recognition of a γδ T cell receptor toward a foreign antigen [0.03%]
类似抗体的γδT细胞受体识别外来抗原方式
Liam Rashleigh,Hariprasad Venugopal,Michael T Rice et al.
Liam Rashleigh et al.
The antigen recognition principles of B cells and αβ T cells have been well described compared to those of the γδ T cell. By way of their specificity conferring receptor (γδTCR), γδ T cells can directly bind proteinaceous antigens. ...
Jack P Connor,Sheena E Radford,David J Brockwell
Jack P Connor
Over 500 amyloid structures have been solved to date to near-atomic resolution. This has highlighted an enormous diversity of fibril structures conforming to the canonical cross-β amyloid fold. Using α-synuclein and tau amyloid structures...
Melanie Schneider,José Antonio Marquez,Andrew R Leach
Melanie Schneider
Proteins exhibit conformational dynamics that underpin function and inform drug design. EnsembleFlex is a computational suite to extract, quantify, and visualize conformational heterogeneity from experimentally determined structure ensemble...
Automated identification of small molecules in cryoelectron microscopy data with density- and energy-guided evaluation [0.03%]
基于密度和能量引导评估的冷冻电子显微镜数据中小分子的自动识别方法研究
Andrew Muenks,Daniel P Farrell,Guangfeng Zhou et al.
Andrew Muenks et al.
Methodological improvements in cryoelectron microscopy (cryo-EM) have made it useful in ligand-bound structure determination for biology and drug design. However, determining ligand conformation and identity is challenging at the resolution...
Cross-species recognition of squirrel ACE2 by the receptor binding domains of SARS-CoV-2, RaTG13, PCoV-GD and PCoV-GX [0.03%]
SARS-CoV-2、RaTG13、PCoV-GD和PCoV-GX的受体结合域识别松鼠ACE2蛋白质研究
Chenghai Wang,Xiaoyan Nan,Yang Deng et al.
Chenghai Wang et al.
SARS-CoV-2 has a broad animal host range, but its origin and intermediate hosts are still debated. Arctic ground squirrel is proved to be a susceptible animal host of SARS-CoV-2 and RaTG13, as the Arctic ground squirrel ACE2 (sACE2) could b...
Regulatory mechanisms of PP2A complex assembly driven by physicochemical differences in A-subunit isoforms [0.03%]
由A亚基异构体的物理化学差异驱动的PP2A复合物装配的调节机制
Alexander Day,Wei Huang,Daniel Leonard et al.
Alexander Day et al.
Protein phosphatase 2A (PP2A) is crucial for regulating cellular pathways, with its holoenzyme assembly affecting enzyme function and substrate selection. The PP2A holoenzyme comprises scaffold A-, regulatory B-, and catalytic C-subunits, e...