Modular protein scaffold architecture and AI-guided sequence optimization facilitate de novo metalloenzyme engineering [0.03%]
模块化蛋白质支架结构及人工智能引导的序列优化可促进从头金属酶工程
Paula Wagner Egea,Florent Delhommel,Ghulam Mustafa et al.
Paula Wagner Egea et al.
Incorporating metal cofactors into computationally designed protein scaffolds provides a versatile route to novel protein functions, including the potential for new-to-nature enzyme catalysis. However, a major challenge in protein design is...
Structure of a SLC26 Anion Transporter STAS Domain in Complex with Acyl Carrier Protein: Implications for E. coli YchM in Fatty Acid Metabolism [0.03%]
SLC26阴离子转运蛋白STAS结构域与酰基载体蛋白复合物的结构：对大肠杆菌YchM在脂肪酸代谢中作用的意义
Mohan Babu,Jack F Greenblatt,Andrew Emili et al.
Mohan Babu et al.
Structural basis of hydride and proton transfer reactions revealed by the detection of hydrogen atoms in mammalian NADH-cytochrome b5 reductase [0.03%]
通过检测哺乳动物NADH细胞色素b5还原酶中氢原子揭示 hydration 和质子转移反应的结构基础
Yu Hirano,Kazuo Kurihara,Katsuhiro Kusaka et al.
Yu Hirano et al.
Many structural studies have been reported for ferredoxin:NADP+ reductase family members, but an experimental validation of the catalytic hydride and proton transfer steps through a direct detection of the involved hydrogen atoms has not be...
Structural basis of VAChT inhibition by spiroindolines and alkylsulfones [0.03%]
.spiroindoline和烷基砜抑制VAChT的结构基础
Yang Zhang,Zhe Zhang
Yang Zhang
Cholinergic signaling relies on the vesicular acetylcholine transporter (VAChT) to package acetylcholine (ACh) into synaptic vesicles. VAChT dysfunction is implicated in neurological and neuromuscular disorders, including Alzheimer's diseas...
Structural analysis of a Gram-positive type VII ABC transporter induced by cell wall-targeting antibiotics [0.03%]
细胞壁靶向抗生素诱导的革兰氏阳性菌第VII型ABC转运蛋白的结构分析
Peixuan Yu,Bradon S Krah,Melanie A Orlando et al.
Peixuan Yu et al.
Bacteria utilize a variety of mechanisms to remodel the cell wall in response to environmental and antimicrobial stress. In the model organism Bacillus subtilis, the ytr operon encoding putative ATP-binding cassette (ABC) transporter(s) is ...
High throughput mutational characterization of the GPCR ligand C5a using yeast display and deep sequencing [0.03%]
基于酵母展示和高通量测序的G蛋白偶联受体配体C5a突变分析
Yu Xu,Kaushik Thakkar,Li Guan et al.
Yu Xu et al.
High-throughput mutagenesis approaches are widely employed to systematically characterize protein functions and play a critical role in therapeutic developments. As the largest class of membrane receptors, G protein-coupled receptors (GPCRs...
Subtle changes in ligand-receptor interactions dramatically alter transcriptional outcomes of pregnane X receptor modulators [0.03%]
配体-受体相互作用的细微变化可显著改变孕烷X受体调节剂的转录结果
Andrew D Huber,Efren Garcia-Maldonado,Wenwei Lin et al.
Andrew D Huber et al.
Nuclear receptor antagonists are used to treat various diseases, but the precise antagonist mechanisms differ among receptors and compounds. Understanding the interplay between ligand-receptor interactions and transcriptional outcomes is cr...
Structure of nanobody-inhibited state of human bile salt transporter NTCP [0.03%]
人胆盐转运蛋白NTCP的纳米抗体抑制态结构
Daniel Yoon,Kamil Nosol,Ali Rasouli et al.
Daniel Yoon et al.
Sodium-taurocholate co-transporting polypeptide (NTCP) is a sodium-dependent transporter mediating the hepatic uptake of bile salts and serving as the receptor of hepatitis B and D viruses. While previous studies identified binding sites fo...
Cryo-EM structure of the RfxCas13d-crRNA-off-target-RNA complex [0.03%]
RfxCas13d-crRNA脱靶RNA复合物的冷冻电镜结构
Qianxi Yang,Yifang Sun,Lei Sun et al.
Qianxi Yang et al.
The CRISPR-Cas system is crucial for the adaptive immune response of prokaryotes and has been widely applied for genetic engineering. Cas13d, a type VI-D CRISPR-Cas effector, functions as RNA-guided ribonuclease and has been engineered for ...
Guojin Ou,Qingting Liu,Haizhan Jiao et al.
Guojin Ou et al.
The subcortical maternal complex (SCMC) is essential for mammalian preimplantation development, yet how SCMCcore (MATER/NLRP5, TLE6, FLOPED/OOEP) engages regulatory partners remains unclear. We determined cryo-EM structures of mouse SCMC bo...