Conformational and dynamic properties of the KH1 domain of FMRP and its fragile X syndrome linked G266E variant [0.03%]
脆性X综合征KH1结构域及其G266E突变体的构象与动态性质研究
Flavia Catalano,Daniele Santorelli,Alessandra Astegno et al.
Flavia Catalano et al.
The Fragile X messenger ribonucleoprotein (FMRP) is a multi-domain protein involved in interactions with various macromolecules, including proteins and coding/non-coding RNAs. The three KH domains (KH0, KH1 and KH2) within FMRP are recogniz...
The apo-acyl coenzyme A binding protein of Leishmania major forms a unique 'AXXA' motif mediated dimer [0.03%]
利什曼原虫的游离酰基辅酶a结合蛋白形成一个独特的由"AXXA"基序介导的二聚体
Shalini Verma,Rohit Singh Dangi,Manoj Kumar Rajak et al.
Shalini Verma et al.
Acyl-Coenzyme A binding domain containing proteins (ACBDs) are ubiquitous in nearly all eukaryotes. They can exist as a free protein, or a domain of a large, multidomain, multifunctional protein. Besides modularity, ACBDs also display multi...
Characterization of AICAR transformylase/IMP cyclohydrolase (ATIC) bifunctional enzyme from Candidatus Liberibacer asiaticus [0.03%]
稻粒肿原体AICA转酰基酶/次黄嘌呤-鸟嘌呤核苷酸磷酸核糖转移酶(ATIC)双功能酶的鉴定
Sapna Lonare,Surabhi Rode,Preeti Verma et al.
Sapna Lonare et al.
The bifunctional enzyme, 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) transformylase/inosine monophosphate (IMP) cyclohydrolase (ATIC) is involved in catalyzing penultimate and final steps of purine de novo biosynthetic pathway cru...
A role of methionines in the functioning of oxidatively modified fibrinogen [0.03%]
甲硫氨酸在氧化修饰纤维蛋白原功能中的作用
L V Yurina,A D Vasilyeva,E S Gavrilina et al.
L V Yurina et al.
Posttranslational modifications in fibrinogen resulting from induced oxidation or oxidative stress in the organism can have deleterious influence on optimal functioning of fibrinogen, causing a disturbance in assembly and properties of fibr...
Epitope mapping of SARS-CoV-2 RBDs by hydroxyl radical protein footprinting reveals the importance of including negative antibody controls [0.03%]
SARS-CoV-2 RBD表位图谱的羟基 radicals 蛋白质足迹法测定：包含阴性抗体对照的重要性不容忽视
Daniel Nyberg Larsen,Jakub Zbigniew Kaczmarek,Yaseelan Palarasah et al.
Daniel Nyberg Larsen et al.
Understanding protein-protein interactions is crucial for drug design and investigating biological processes. Various techniques, such as CryoEM, X-ray spectroscopy, linear epitope mapping, and mass spectrometry-based methods, can be employ...
Active roles of lytic polysaccharide monooxygenases in human pathogenicity [0.03%]
溶解性多糖单加氧酶在人类病原体中的积极作用
Daniel Kracher,Tina Lanzmaier,Leonor Vieira Carneiro
Daniel Kracher
Lytic polysaccharide monooxygenases (LPMOs) are redox enzymes widely studied for their involvement in microbial and fungal biomass degradation. The catalytic versatility of these enzymes is demonstrated by the recent discovery of LPMOs in a...
Human apo-metallothionein 1a is not a random coil: Evidence from guanidinium chloride, high temperature, and acidic pH unfolding studies [0.03%]
人类apo金属硫蛋白1a不是随机线圈的证据：来自瓜胺氯化物、高温和酸性ph变性研究的结果
Natalie C Korkola,Martin J Stillman
Natalie C Korkola
The structures of apo-metallothioneins (apo-MTs) have been relatively elusive due to their fluxional, disordered state which has been difficult to characterize. However, intrinsically disordered protein (IDP) structures are rather diverse, ...
Structural basis for the allosteric behaviour and substrate specificity of Lactococcus lactis Prolidase [0.03%]
Lactococcus乳酸菌脯氨酸二肽酶别构行为及底物特异性的作用机制基础
Shangyi Xu,Pawel Grochulski,Takuji Tanaka
Shangyi Xu
Prolidase (EC 3.4.13.9) is an enzyme that specifically hydrolyzes Xaa-Pro dipeptides into free amino acids. We previously studied kinetic behaviours and solved the crystal structure of wild-type (WT) Lactococcus lactis prolidase (Llprol), s...
Biophysical characterization of human-cell-expressed, full-length κI O18/O8, AL-09, λ6a, and Wil immunoglobulin light chains [0.03%]
人细胞表达的全长κI O18/O8, AL-09, λ6a和Wil免疫球蛋白轻链的生物物理特征分析
Pinaki Misra,Alexander Tischer,Lindsey Lampe et al.
Pinaki Misra et al.
Immunoglobulin light chain (AL) amyloidosis involves the deposition of insoluble monoclonal AL protein fibrils in the extracellular space of different organs leading to dysfunction and death. Development of methods to efficiently express an...
Characterisation of the forest cobra (Naja melanoleuca) venom using a multifaceted mass spectrometric-based approach [0.03%]
利用多种质谱法对森林眼镜蛇（Naja melanoleuca）的毒液进行特征分析
C Ruth Wang,Alix C Harlington,Marten F Snel et al.
C Ruth Wang et al.
Snake venoms consist of highly biologically active proteins and peptides that are responsible for the lethal physiological effects of snakebite envenomation. In order to guide the development of targeted antivenom strategies, comprehensive ...