Development and validation of an improved diced electrophoresis gel assay cutter-plate system for enzymomics studies [0.03%]
一种用于酶组学研究的改进型切胶电泳胶片测定系统的建立及验证
Toru Komatsu,Masahiro Shimoda,Yukiko Kawamura et al.
Toru Komatsu et al.
Diced electrophoresis gel (DEG) assay is a methodology to identify enzymes with a specified activity in complex cell or tissue lysates by means of two-dimensional separation using isoelectric focusing and native PAGE, followed by dicing of ...
Phosphoproteomic identification and functional characterization of protein kinase substrates by 2D-DIGE and Phos-tag PAGE [0.03%]
基于二维差异凝胶电泳及 Phos-tag 梯度聚丙烯酰胺凝胶电泳的磷酸化蛋白质组学分析及功能鉴定研究
Kou Motani,Hidetaka Kosako
Kou Motani
Protein phosphorylation is one of the most common post-translational modifications in eukaryotes and can regulate diverse properties of proteins. Protein kinases are encoded by more than 500 genes in higher eukaryotes and play central roles...
Bioenergetic and proteomic profiling to screen small molecule inhibitors that target cancer metabolisms [0.03%]
用于靶向癌症代谢的小分子抑制剂的生物能和蛋白质组学筛选
Yushi Futamura,Makoto Muroi,Harumi Aono et al.
Yushi Futamura et al.
Cancer cells can reprogram their metabolic machinery to survive. This altered metabolism, which is distinct from the metabolism of normal cells, is thought to be a possible target for the development of new cancer therapies. In this study, ...
Increase in constitutively active MEK1 species by introduction of MEK1 mutations identified in cancers [0.03%]
癌症中发现的MEK1突变体的引入增加了一种组成性活化的MEK1物种
Emiko Kinoshita-Kikuta,Eiji Kinoshita,Sayaka Ueda et al.
Emiko Kinoshita-Kikuta et al.
The kinase MEK1 is an essential component of the mitogen-activated protein kinase cascades. Somatic mutations that have been identified in the MEK1-coding gene generally enhance kinase activity. Consequently, MEK1 has attracted much interes...
A rapid separation and characterization of mucins from mouse submandibular glands by supported molecular matrix electrophoresis [0.03%]
基于分子矩阵的支持电解法对小鼠颌下腺粘蛋白的快速分离与表征
Akihiko Kameyama,Kimi Yamakoshi,Atsushi Watanabe
Akihiko Kameyama
Mucins are heavily glycosylated proteins with high molecular mass, and are involved in various diseases including infection, inflammation, and cancer. As easy separation method, such as gel electrophoresis, however, does not exist for mucin...
A method for profiling the phosphorylation state of tyrosine protein kinases [0.03%]
一种酪氨酸蛋白激酶磷酸化状态的分析方法
Yuuki Uezato,Isamu Kameshita,Keiko Morisawa et al.
Yuuki Uezato et al.
Protein kinases are known to be implicated in various biological phenomena and diseases through their involvement in protein phosphorylation. Therefore, analysis of the activity of protein kinases by examination of their phosphorylation sta...
Prognostic significance of G6PD expression and localization in lung adenocarcinoma [0.03%]
G6PD表达及定位对肺腺癌预后的意义
Ryo Nagashio,Shota Oikawa,Kengo Yanagita et al.
Ryo Nagashio et al.
Abnormal expressions of extracellular matrix (ECM) proteins are correlated with increased tumor progression, an advanced histologic grade, and metastasis. LCN1 cells derived from a pulmonary large cell neuroendocrine carcinoma were grown to...
Effects of salazosulfapyridine on the profile of cell surface proteins, revealed by biotinylation of cell surface proteins and 2-dimentional electrophoresis [0.03%]
细胞表面生物素标记和二维电泳揭示的柳氮磺胺吡啶对细胞表面蛋白谱型的影响
Kazuki Omoteyama,Toshiyuki Sato,Mitsumi Arito et al.
Kazuki Omoteyama et al.
Objective: We investigated effects of salazosulfapyridine (SASP) on the protein profile of cell surface (CS)-proteins of SW982, a human synovial sarcoma cell line, using biotinylation of CS-proteins and 2-dimensional fluo...
Hiroyuki Yokota
Hiroyuki Yokota
The significance of proteomics in the pharmaceutical industry has increased since overcoming initial difficulties. This review discusses recent proteomics publications from pharmaceutical companies to identify new trends in proteomics appli...