Full-size recombinant ORF1p-L1 and RT domain of ORF2p-L1: Protein expression, purification and characterization [0.03%]
全长ORF1p-L1和ORF2p-L1 RT结构域的蛋白质表达、纯化及鉴定
Vladislav Aksenov,Maria G Glazunova,Igor P Oscorbin et al.
Vladislav Aksenov et al.
Long interspersed nuclear elements-1 (LINEs-1) are known to be active human retrotransposons containing two protein-coding genes, ORF1 and ORF2, whose expression results in the production of two proteins-ORF1p-L1 and ORF2p-L1, respectively....
Identification of novel biomolecular characteristics and bioinformatic analyses of the anterior cingulate cortex in morphine-dependent mice via proteomic profiling [0.03%]
基于成瘾小鼠纹状区蛋白组学特征的生物标志物筛选及验证研究
Jun-Xia Yang,Song Yao,Hong-Wei Cao et al.
Jun-Xia Yang et al.
Impaired function of the anterior cingulate cortex (ACC) is widely recognized as a critical factor in drug dependence. This study aimed to investigate protein alterations in the ACC of morphine-dependent mice using 4D label-free quantitativ...
Electrochemical enzyme biosensing reveals differential abundance of GPI-anchored metalloprotease across Leishmania (Viannia) braziliensis subpopulations [0.03%]
电化学酶生物传感揭示Leishmania(Viannia)braziliensis亚群之间的锚定金属蛋白酶的差异性丰度
Fatemeh Farshchi,Geovane Dias-Lopes,Vitor Ennes-Vidal et al.
Fatemeh Farshchi et al.
Leishmania (Viannia) braziliensis Thor strain contains subpopulations Thor03, Thor10, and Thor22 with distinct biological and infection profiles in vitro and vivo. This study investigated GPI-anchored metalloproteases from the Thor strain a...
MS analysis of proteins concentrated on the surface of AFM chips from the blood plasma of healthy volunteers [0.03%]
健康志愿者血浆AFM芯片表面集中的蛋白质的质谱分析
Arina I Gordeeva,Anastasia A Valueva,Tatyana A Materova et al.
Arina I Gordeeva et al.
Proteomic analysis of blood plasma plays a crucial role in biomedical research for biomarker discovery, development of diagnostic systems, and monitoring of treatment efficacy. In this study, we combine mass spectrometric analysis with surf...
Chemometric evaluation of key residues affecting the stability of cold shock proteins [0.03%]
冷休克蛋白稳定性的关键残基的化学计量学评价
Jack E Buckley,Nathaniel J Zbacnik,Charles S Henry et al.
Jack E Buckley et al.
Reduced properties, also known as amino acid descriptors, quantify changes in physicochemical properties of amino acids upon mutation. Combined with PLS (partial least squares) modeling, the impact of various mutations on the conformational...
Purification of human sperm-specific PGK2 and assay development for drug screening for potential non-hormonal contraceptives [0.03%]
人特异性精子PGK2的纯化及潜在非激素类避孕药物筛选检测方法的研发
Samprikta Kundu,Safia Baig,Ariba Mahmood et al.
Samprikta Kundu et al.
Biochemical characterization of a glycoside hydrolase family 10 β-galactosidase from a haloalkaliphilic archaeon [0.03%]
来自耐盐嗜碱性的古菌的β-半乳糖苷酶（糖基水解酶家族10）的生化表征
Nawee Jantarit,Chamaipon Beagbandee,Yothin Teethaisong et al.
Nawee Jantarit et al.
Extremophilic archaea have garnered attention as valuable sources of robust enzymes appropriate for industrial applications. This study involved the cloning and heterologous expression of a gene initially designated as encoding a putative g...
Cytotoxicity originates at the amorphous intermediate stage during amyloid-like oligomerization of serum albumin under mild denaturing conditions [0.03%]
在温和变性条件下，血清白蛋白类淀粉样低聚化过程中无定形中间体阶段产生细胞毒性
Gulafsha,Rinshu Singh,Geeta Arya et al.
Gulafsha et al.
Protein misfolding and aggregation into amyloid-like structures cause many pathological conditions and remain central to understanding protein homeostasis. Here, we report mechanistic insights into the aggregation of human serum albumin (HS...
Calcium dependence and biochemical characterization of metacaspase-3 from Trypanosoma cruzi [0.03%]
钙离子依赖性和生化表征美洲钩虫metacaspase-3
Ane Caroline Moreira Duarte,Vinicius Henrique de Oliveira,João Pedro Martins Silva Costa et al.
Ane Caroline Moreira Duarte et al.
Metacaspases are cysteine proteases structurally related to caspases, widely distributed in plants, fungi, and protozoa, but absent in metazoans. In Trypanosoma cruzi, the etiological agent of Chagas disease, the functional and biochemical ...
Multifunctionality analysis of serine hydroxymethyltransferases from human and Escherichia coli [0.03%]
人和大肠杆菌丝氨酸羟甲基转移酶多功能性分析
Mahiro Hayashi,Kumiko Sakai-Kato,Tetsuya Miyamoto
Mahiro Hayashi
Serine hydroxymethyltransferase (SHMT) catalyzes the conversion of l-serine and tetrahydrofolate (THF) to glycine and 5,10-methylenetetrahydrofolate, respectively. In a previous study, we found that human and Escherichia coli SHMTs possess ...