From sequence to function: Exploring biophysical properties of bacteriophage BFK20 lytic transglycosylase domain from the minor tail protein gp15 [0.03%]
从序列到功能：研究噬菌体BFK20次要尾部蛋白gp15裂解转糖基酶结构域的生物物理性质
Kristina Papayova,Lucia Bocanova,Vladena Bauerova et al.
Kristina Papayova et al.
Bacteriophages have evolved different mechanisms of infection and penetration of bacterial cell walls. In Siphoviridae-like viruses, the inner tail proteins have a pivotal role in these processes and often encode lytic protein domains which...
Assigning roles in Chlamydomonas ribosome biogenesis: The conserved factor NIP7 [0.03%]
绿藻叶绿体中的核糖体生物发生：保守因子NIP7的作用机制研究
Raissa Ferreira Gutierrez,Heloisa Ciol,Angélica L Carrillo Barra et al.
Raissa Ferreira Gutierrez et al.
Ribosome biogenesis (RB) is a highly conserved process across eukaryotes that results in the assembly of functional ribosomal subunits. Studies in Saccharomyces cerevisiae and Homo sapiens have identified numerous RB factors (RBFs), includi...
Identification of potential pharmacological chaperones that selectively stabilize mutated Aspartoacylases in Canavan disease [0.03%]
卡那万病潜在药物伴侣的鉴定及其选择性稳定突变天冬氨酰胺酶的作用
Nitesh Kumar Poddar,Yasanandana S Wijayasinghe,Ronald E Viola
Nitesh Kumar Poddar
Canavan disease is caused by mutations in the ASPA gene, leading to diminished catalytic activity of aspartoacylase in the brain. Clinical missense mutations are found throughout the enzyme structure, with many of these mutated enzymes havi...
Kinetic characterization of the C-terminal domain of Malonyl-CoA reductase [0.03%]
丙二酰辅酶A还原酶C末端结构域的动态特征研究
Mirela Tkalcic Cavuzic,Amanda Silva de Sousa,Jeremy R Lohman et al.
Mirela Tkalcic Cavuzic et al.
Malonyl-CoA reductase utilizes two equivalents of NADPH to catalyze the reduction of malonyl-CoA to 3-hydroxypropionic acid (3HP). This reaction is part of the carbon fixation pathway in the phototrophic bacterium Chloroflexus aurantiacus. ...
The regulation of the thermal stability and affinity of the HSPA5 (Grp78/BiP) by clients and nucleotides is modulated by domains coupling [0.03%]
客户蛋白和核苷酸通过结合域耦合调节HSPA5(Grp78/BiP)的热稳定性和亲和力
Noeli S M Silva,Bruna Siebeneichler,Carlos S Oliveira et al.
Noeli S M Silva et al.
The HSPA5 protein (BiP/Grp78) serves as a pivotal chaperone in maintaining cellular protein quality control. As a member of the human HSP70 family, HSPA5 comprises two distinct domains: a nucleotide-binding domain (NBD) and a peptide-bindin...
Co-chaperonin GroES subunit exchange as dependent on time, pH, protein concentration, and urea [0.03%]
协同分子GroES亚基交换取决于时间、PH值、蛋白质浓度和尿素的影响
Victor Marchenkov,Alexey Surin,Victor Ugarov et al.
Victor Marchenkov et al.
The discovery of a subunit exchange in some oligomeric proteins, implying short-term dissociation of their oligomeric structure, requires new insights into the role of the quaternary structure in oligomeric protein stability and function. H...
Mapping the Periostin splice isoforms in atopic dermatitis and an in vitro asthma model - A multi-platform analysis using mass spectrometry and RT-qPCR [0.03%]
基于质谱和RT-qPCR的多平台分析在特应性皮炎及体外哮喘模型中映射骨桥蛋白剪切异构体分布
Christian E Rusbjerg-Weberskov,Anne Kruse Hollensen,Christian Kroun Damgaard et al.
Christian E Rusbjerg-Weberskov et al.
Periostin is a matricellular protein known to be alternatively spliced to produce ten isoforms with a molecular weight of 78-91 kDa. Within the extracellular matrix, periostin attaches to cell surfaces to induce signaling via integrin-bindi...
Robust assessment of sample preparation protocols for proteomics of cells and tissues [0.03%]
用于细胞和组织蛋白质组学研究的样品前处理方案的效果评估
Francielle Aguiar Gomes,Douglas Ricardo Souza Junior,Mariana Pereira Massafera et al.
Francielle Aguiar Gomes et al.
In proteomic studies, the reliability and reproducibility of results hinge on well-executed protein extraction and digestion protocols. Here, we systematically compared three established digestion methods for macrophages, namely filter-assi...
Exploring liquid-liquid phase separation in the organisation of Golgi matrix proteins [0.03%]
液-液相分离在高尔基体矩阵蛋白组织中的作用探索
Luis Felipe S Mendes,Carolina G Oliveira,Kevin F Simões et al.
Luis Felipe S Mendes et al.
The Golgi apparatus is a critical organelle in protein sorting and lipid metabolism. Characterized by its stacked, flattened cisternal structure, the Golgi exhibits distinct polarity with its cis- and trans-faces orchestrating various prote...
Plumbagin accelerates serum albumin's amyloid aggregation kinetics and generates fibril polymorphism by inducing non-native β-sheet structures [0.03%]
白花丹素通过诱导非天然β折叠结构加速血清白蛋白淀粉样蛋白聚集并产生原纤维多态性
Chanchal Chauhan,Poonam Singh,Shivani A Muthu et al.
Chanchal Chauhan et al.
The ligand-induced conformational switch of proteins has great significance in understanding the biophysics and biochemistry of their self-assembly. In this work, we have investigated the ability of plumbagin (PL), a hydroxynaphthoquinone c...