John T Poirier
John T Poirier
CRISPR-Cas9 technology has revolutionized large-scale functional genomic screening in mammalian cell-culture systems. Due in part to optimized lentiviral delivery vectors; it is now possible to perform CRISPR-Cas9 screens in animals in orde...
Dynamics of Indel Profiles Induced by Various CRISPR/Cas9 Delivery Methods [0.03%]
由不同CRISPR / Cas9递送方法诱导的插入和缺失谱的动力学变化规律
Michael Kosicki,Sandeep S Rajan,Flaminia C Lorenzetti et al.
Michael Kosicki et al.
The introduction of CRISPR/Cas9 gene editing in mammalian cells is a scientific breakthrough, which has greatly affected basic research and gene therapy. The simplicity and general access to CRISPR/Cas9 reagents has in an unprecedented mann...
Marta Martinez-Lage,Raúl Torres-Ruiz,Sandra Rodriguez-Perales
Marta Martinez-Lage
The CRISPR/Cas9 system development has revolutionized the field of genome engineering through the efficient creation of targeted breaks in the DNA of almost any organism and cell type, opening an avenue for a wide range of applications in b...
Gene Editing and CRISPR Therapeutics: Strategies Taught by Cell and Gene Therapy [0.03%]
基因编辑及CRISPR药物治疗：来自细胞和基因治疗的策略启示
Juan C Ramirez
Juan C Ramirez
A few years ago, we assisted in the demonstration for the first time of the revolutionary idea of a type of adaptive-immune system in the bacteria kingdom. This system, named CRISPR, and variants engineered in the lab, have been demonstrate...
Wenyuan Han,Qunxin She
Wenyuan Han
CRISPR research is a very young research field since it was only 10years ago when the system was found to confer antiviral defense. Nevertheless, there has been an explosion of publications in CRISPR research in the past 5years. The researc...
Pregnancy and Breast Cancer [0.03%]
妊娠与乳腺癌
Ramadevi Subramani,Rajkumar Lakshmanaswamy
Ramadevi Subramani
Breast cancer is the most commonly diagnosed type of cancer among women worldwide. The majority of breast cancers are sporadic and the etiology is not well understood. Several factors have been attributed to altering the risk of breast canc...
Current Approaches to Diagnosis and Treatment of Ductal Carcinoma In Situ and Future Directions [0.03%]
当前的导管原位癌诊断和治疗思路及未来方向
Randi Ryan,Ossama Tawfik,Roy A Jensen et al.
Randi Ryan et al.
The presentation and treatment of ductal carcinoma in situ (DCIS) has changed substantially over the years. While previously an incidental pathologic finding in more advanced, palpable tumors, the institution of screening mammography has re...
Ramadevi Subramani,Rajkumar Lakshmanaswamy
Ramadevi Subramani
Complementary and alternative medicine (CAM) has been in use among cancer patients for a long time. There are several types of CAM that are practiced in various parts of the world. For example, traditional medicinal practices followed in In...
Microbiota and Breast Cancer [0.03%]
微生物群与乳腺癌
Sridhar Mani
Sridhar Mani
Breast carcinogenesis and/or cancer growth and/or drug sensitivity has a multifactorial etiology-perhaps the least well-characterized aspect being that of the distant environmental influences, namely, the microbiota that inhabit humans. For...