Vijai Singh
Vijai Singh
Ingita Dey Munshi,Indra Mani
Ingita Dey Munshi
Cell-free systems let researchers carry out biological processes like protein synthesis and metabolism without using living cells. This approach has become increasingly important in synthetic biology because it allows for quick testing of i...
Mansi Acharya,Indra Mani
Mansi Acharya
Cell-free systems (CFS) decouple gene expression and metabolic pathways from living cells, offering a rapid, modular platform for biosensing, pathway prototyping, and protein production. This review surveys mechanistic and data-driven compu...
Ajay Kumar,Juveriya Israr,Shabroz Alam
Ajay Kumar
Cell-free transcription-translation (TXTL) systems provide a robust platform for in vitro protein synthesis, transforming molecular biology, synthetic biology, and biotechnology by replicating natural protein synthesis outside of living cel...
Non-canonical amino acid incorporation via genetic code reprogramming in a cell-free translation system [0.03%]
基于无细胞系统遗传密码重编程的非标准氨基酸蛋白质翻译体外引入技术研究进展
Harinarayana Ankamareddy,Hemasundar Alavilli,Vini Madathil et al.
Harinarayana Ankamareddy et al.
Non-canonical amino acids (ncAAs) and unnatural amino acids (UAAs) both holds the characteristic features of the amino acids but lacks functional role in the protein synthesis. The ncAAs and UAAs possess unique chemical, physical or biologi...
Mohd Tariq,Nil Patil,Mukul Jain et al.
Mohd Tariq et al.
Cell-free systems (CFS) have emerged as transformative tools in synthetic biology, enabling the execution of complex biological reactions such as transcription and translation outside the confines of living cells. By eliminating the cellula...
B G Pavana,G Sowmiya,Sai Ramesh et al.
B G Pavana et al.
Cell-free protein synthesis (CFPS) has emerged as a revolutionary process for producing proteins quickly and in a modular way, with the optionality of not having all of the restraints of living cells. As described in this chapter, we provid...
Brahmjot Singh,Jyoti,Suhail Kapta et al.
Brahmjot Singh et al.
High-throughput screening (HTS) has revolutionized the identification and evaluation of biomolecules by enabling the parallel testing of large libraries of compounds, nucleic acids, and proteins against biological targets. Traditionally con...
Rupal Dhariwal,Mukul Jain
Rupal Dhariwal
Cell-free systems have also become a revolutionary platform for low-cost diagnostics, providing fast, flexible, and scalable solutions to the conventional cell-based assays. Such systems, which utilize the fundamental biochemical machinery ...
Stuti Ganatra,Alok Pandya
Stuti Ganatra
Cell-free systems (CFSs) have become powerful tools in synthetic biology, enabling the creation of fast, modular, and customizable biosensors without relying on living cells. By utilizing in vitro transcription and translation, these system...