AI tools for cryo-EM: Protein particle picking, density map enhancement, and atomic model building [0.03%]
用于cryo-em的AI工具：蛋白颗粒挑选、密度图增强和原子模型构建
Ashwin Dhakal,Rajan Gyawali,Joel Selvaraj et al.
Ashwin Dhakal et al.
Single-particle cryo-electron microscopy (cryo-EM) has rapidly evolved from largely manual, stepwise workflows to AI-assisted, increasingly automated protein structure determination pipelines. This chapter presents an end-to-end view on our...
Joel Yeo,N Duane Loh
Joel Yeo
Cryo-electron microscopy (cryo-EM) has revolutionized structural biology, enabling near-atomic resolution determination of biological macromolecules. The success of modern reconstruction algorithms relies heavily on simplified image formati...
Overview and future potential in structural studies of bacteriophages by cryogenic electron microscopy [0.03%]
低温电子显微镜在噬菌体结构研究中的应用及其潜力
Michael Sherman,Elena Orlova
Michael Sherman
Recent advances in biological specimen preparation, electron optics, and image processing software have led to explosive growth in structural biology, in particular, in bacteriophage structural studies in situ. Bacteriophages are microscopi...
Microcrystal electron diffraction (MicroED) for high-resolution structure determination [0.03%]
用于高分辨率结构测定的微晶电子衍射（MicroED）技术
Brent L Nannenga,Michael W Martynowycz
Brent L Nannenga
Microcrystal electron diffraction (MicroED) has emerged as a robust approach for determining structures of biological macromolecules, small organic molecules and materials at high resolution. MicroED acquires electron diffraction data from ...
GoToCloud: Development and future of the cloud-based platform for cryo-EM structure-based drug DesignDevelopment and future of GoToCloud [0.03%]
基于云的结构基础药物设计平台GoToCloud的开发及其未来
Toshio Moriya
Toshio Moriya
The "Resolution Revolution" in cryogenic electron microscopy (Cryo-EM) has fundamentally advanced structure-based drug design (SBDD), moving beyond static snapshots to dynamic conformational analysis. However, the resulting exponential grow...
Recent cryo-EM structures redefine the functional amyloid landscape [0.03%]
近期的冷冻电镜结构重新定义了功能淀粉样蛋白的分布格局
Salvador Ventura,Andrea Bartolomé-Nafría,Javier Garcia-Pardo
Salvador Ventura
Amyloids are traditionally associated with neurodegenerative and systemic diseases, yet it is now clear that they also perform essential physiological functions across kingdoms of life. Over the last decade, advances in cryogenic electron m...
Insights into the structure and diversity of the bacterial flagellum from cryo-EM studies [0.03%]
冷冻电镜研究揭示细菌鞭毛的结构和多样性新见解
Kailin Qin,Matías R Iglesias Rando,Julien R C Bergeron
Kailin Qin
The bacterial flagellum is a large proteinaceous macromolecular machine that enables motility, host interaction, and virulence across bacterial species. Cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) have reshaped...
Calcium-sensing receptor involved in the oral perception of kokumi substances [0.03%]
钙感受器与 Kokumi 物质的口腔感知有关
Hiroki Yamaguchi,Seiji Kitajima
Hiroki Yamaguchi
Taste is a key determinant of food preference and involves basic tastes and complex sensations such as mouthfeel. To understand taste at a molecular level, researchers have used cryo-electron microscopy (cryo-EM) to determine the structures...
Computational approaches for protein complex modeling for intermediate resolution cryo-EM maps [0.03%]
中间分辨率冷冻电镜图谱的蛋白质复合物建模计算方法
Joon Hong Park,Genki Terashi,Daisuke Kihara
Joon Hong Park
Cryogenic electron microscopy (cryo-EM) has become a central technology for determining macromolecular structures in structural biology. However, interpreting density maps by structure modeling from intermediate (∼5-10Å) to low resolution...