Refined annexin V/PI apoptosis protocol to minimize false-positive necrotic cells [0.03%]
一种优化的Annexin V/PI凋亡检测方案以减少假阳性坏死细胞
Aurora Schiavon,Carlotta Evaristo,Laura Saba et al.
Aurora Schiavon et al.
Programmed cell death (PCD) is a fundamental biological process required for tissue homeostasis and the removal of damaged or infected cells. Apoptosis, one of the most studied PCD modalities, is routinely assessed using the Annexin V/propi...
Modulating amyloid-β 42 aggregation and neurotoxicity by Kunitz domains and their derived peptides [0.03%]
利用Kunitz结构域及其衍生多肽调节淀粉样蛋白-β42聚集及神经毒性作用
Maya Rabinovich,Shiran Lacham-Hartman,Niv Papo
Maya Rabinovich
Alzheimer's disease is associated with the aggregation of amyloid-β42 (Aβ42) into species of varying sizes, with intermediate oligomers being the most neurotoxic. We recently reported that amyloid precursor protein inhibitor (APPI), a Kun...
An integrated automated pipeline for cloning, transient expression, and purification augmenting the plant cell pack platform for biopharmaceutical screening [0.03%]
一种整合的自动化流水线克隆、转录表达及纯化技术促进植物细胞包平台用于生物药物筛选
Monique Schulze,Patrick Opdensteinen,Sandor Albert et al.
Monique Schulze et al.
Biopharmaceuticals, particularly monoclonal antibodies (mAbs), are a rapidly expanding class of therapeutics and have benefitted from high-throughput screening (HTS) strategies for protein engineering. Obtaining such complex biopharmaceutic...
Sustainable production of recombinant FGF2 in cell culture spent media using engineered Lactococcus lactis: Modelling and process integration [0.03%]
采用工程化乳酸菌回收细胞培养液中重组FGF2的可持续生产：建模与过程整合
Prashant Mainali,Jin Hao Tan,Jun Ping Quek et al.
Prashant Mainali et al.
Spent mammalian cell culture media are commonly discarded despite having residual nutrients that could be repurposed for microbial biomanufacturing. Fibroblast growth factor 2 (FGF2) is a major cost contributor in serum-free cell culture me...
A compact minimal promoter-feedback circuit enables enhanced oviduct-restricted transgene expression in chickens [0.03%]
一种紧凑的最小启动子反馈电路，可在鸡中实现输卵管特异表达基因增强表达
Yoshinori Kawabe,Takayuki Otsubo,Reina Obata et al.
Yoshinori Kawabe et al.
Precise spatial restriction of transgene expression is essential for safe in vivo production of bioactive proteins; however, regulatory elements that confer tissue specificity often span large genomic regions and are incompatible with size-...
Biparatopic affibody engineering enables high-affinity sortilin blockade and progranulin elevation [0.03%]
双价AFFIBODY工程实现高亲和力SORL1阻断并升高PGRN水平
Moira Ek,Hanna Lindberg,Stefan Ståhl et al.
Moira Ek et al.
Loss-of-function mutations in the gene encoding progranulin (PGRN) are a common cause of frontotemporal dementia, leading to markedly decreased PGRN levels. A potential therapeutic strategy is therefore to increase extracellular PGRN by blo...
Lycopene pathway rewiring by combinatorial co-expression of constituent enzymes in a cell-free protein synthesis system [0.03%]
无细胞蛋白质合成系统中组合表达途径酶重构类胡萝卜素代谢路径
Heawon Lim,Yunbyeong Chae,Dayeon Nam et al.
Heawon Lim et al.
We established a platform for reconstructing the lycopene pathway by combinatorial co-expression of five constituent enzymes using a crude Escherichia coli cell-free protein synthesis (CFPS) system. DNA templates were prepared in a modular ...
Engineering Cytochromes for Photocatalysis: Biohybrid Assemblies for Light-Driven Dye Decoloration [0.03%]
工程化细胞色素用于光催化：用于光驱动脱色的生物杂合组装体
Jessica H van Wonderen,Daisy L Kent,Mary E G Emmerson et al.
Jessica H van Wonderen et al.
A major challenge in developing effective photocatalysts lies in engineering the efficient coupling of one-electron photochemistry with the multi-electron requirements of chemical transformations. Here we demonstrate biohybrid assemblies th...
Fish immunization by duckweed biomass accumulating recombinant cyprinid herpesvirus 3 antigens induces specific immune response [0.03%]
聚集草鱼呼肠孤病毒3型抗原的水生植物 Wolffia arrhiza诱导鱼类特异性免疫反应
Olena Kishchenko,Anton Peterson,Mikolaj Adamek et al.
Olena Kishchenko et al.
Cyprinid herpesvirus-3 (CyHV-3) is highly contagious and lethal to cyprinid fish, necessitating development of sustainable vaccination strategies. Our study explores duckweed as platform for vaccine production and as vehicle for oral admini...
Evaluation of Ogataea polymorpha DUR31 TPP riboswitch as a tool to downregulate gene expression in the yeast Komagataella phaffii [0.03%]
评估毕赤酵母中的Ogataea polymorpha DUR31 TPP核糖开关作为下调基因表达的工具
Paweł Małecki,Brigitte Gasser
Paweł Małecki
Komagataella phaffii (syn. Pichia pastoris) is a methylotrophic yeast widely established as host for recombinant protein production and increasingly used as cell factory for C1-based metabolites. Nevertheless, synthetic biology tools for re...