SPTEdU-seq enables parallel optics-free newborn cell tracking and spatial total transcriptional dynamics in intact microenvironments [0.03%]
SPTEdU-seq可在完整微环境中实现无需光学器件的新出生细胞追踪和空间总转录动态变化的并行光学操作
Haofu Niu,Shuang Zhang,Jizhong Mao et al.
Haofu Niu et al.
Understanding biological processes requires spatiotemporal mapping of proliferative and transcriptional dynamics. Current spatial transcriptomics methods capture only protein-coding transcripts and static snapshots, obscuring non-coding RNA...
Preservation of chondrocyte microspheroids by local sustained hydrogen supply improves osteoarthritic cartilage repair [0.03%]
局部持续供氢保存软骨细胞微球体可改善骨关节炎软骨修复
Shengqiang Chen,Mingrui Luo,Sizhen Chen et al.
Shengqiang Chen et al.
The inflammatory pathological microenvironment of osteoarthritis (OA) degrades the cell state and function of stem cell-derived grafts and presents a major obstacle to developing effective cell therapies. Here, we show that the viability an...
Shuyue Xu,Jingwen Xu,Qiqi Yang et al.
Shuyue Xu et al.
Tumors are increasingly recognized as a consequence of systemic immune dysregulation, while current therapies merely focus on direct tumor killing or local immune activation, overlooking the systemic immune landscape that enables tumorigene...
Human pluripotent stem cell-derived atrioventricular node-like pacemaker cells exhibit biological conduction bridge properties [0.03%]
人多能干细胞来源的房室结类起搏细胞具有生物传导桥功能属性
Michelle Lohbihler,Amos A Lim,Stéphane Massé et al.
Michelle Lohbihler et al.
The atrioventricular node (AVN) ensures synchronized heart contractions by establishing the electrical connection between the atria and ventricles. Dysfunction of the pacemaker cells of the AVN leads to atrioventricular block (AV block), a ...
Metabolite-induced DNA damage drives stochastic stem cell loss and clonal hematopoiesis [0.03%]
代谢物诱导的DNA损伤驱动干细胞随机损耗和克隆造血发生
Ashley N Kamimae-Lanning,Jill M Brown,Matthias Günther et al.
Ashley N Kamimae-Lanning et al.
DNA damage and mutations in hematopoietic stem cells (HSCs) enable clonal hematopoiesis (CH). Such damage occurs across a lifetime, but its origins remain unknown. Here, we demonstrate that endogenous formaldehyde causes HSC attrition and s...
Decoding the spatiotemporal development of the blood-brain barrier in human cortex [0.03%]
解析人类皮层血脑屏障的时空发展过程
Zhongqiu Li,Yanxin Li,Ziqing He et al.
Zhongqiu Li et al.
The blood-brain barrier (BBB) is essential for maintaining the homeostasis of the central nervous system. However, the processes of BBB formation in humans remain unclear. Here, using single-cell spatiotemporal transcriptomics, we investiga...
Auditory activity sustains adult neurogenesis and cognition through the locus coeruleus-norepinephrine system [0.03%]
通过蓝斑-去甲肾上腺素系统维持成人神经发生和认知的听觉活动
Qiang Liu,Xing Luo,Ziqi Liang et al.
Qiang Liu et al.
Hearing loss has been considered as potentially the leading modifiable risk factor for cognitive decline and dementia, but the causal link between these two conditions remains unsolved. Here, we report that mice with specific ablation of co...
Species-specific chromatin architecture and neurogenesis mediated by a human enhancer [0.03%]
特定物种的染色体结构和神经元生成由人类增强子介导
Federica Mosti,Jing Liu,Katie Lam et al.
Federica Mosti et al.
Genomic modifications underlie the evolution of human brain features, including a larger neocortex. Human accelerated regions (HARs) are highly conserved loci containing human-specific variants, with ∼50% identified as neurodevelopmental e...
Paired patient-derived organoids reveal transcription factor-driven epigenetic remodeling in breast cancer metastasis [0.03%]
配对患者衍生类器官揭示了乳腺癌转移中转录因子驱动的表观遗传重塑
Xinxin Rao,Jingwen Wang,Zhibin Qiao et al.
Xinxin Rao et al.
Breast cancer exhibits marked clinical heterogeneity and dynamic epigenetic reprogramming during tumor progression, yet current subtyping approaches fail to capture molecular changes associated with metastasis. Here, we establish a comprehe...
In vivo CAR-Tfh cell reprogramming restores tolerance in a mouse model of autoimmune hepatitis [0.03%]
体内CAR-Tfh细胞重编程可在自身免疫性肝炎小鼠模型中恢复耐受性
Zuolin Zheng,Ying Liu,Ganyu Wang et al.
Zuolin Zheng et al.
Autoimmune diseases involve the coordinated dysregulation of multiple types of immune cells, and single-cell targeted therapies often yield suboptimal results. Here, we developed chimeric antigen receptor (CAR)-engineered follicular helper ...