Expression and Purification of p70 (∆NH2-146/∆CT104) S6 K, a 57.3 kDa N- and C-Terminal Truncated p70S6 Kinase-GST Fusion Protein in Bacterial Expression System [0.03%]
表达及纯化不含N端146氨基酸和C端104氨基酸的p70(S6K) GST融合蛋白（分子量57.3KD）细菌表达体系研究
Shafat A Latoo
Shafat A Latoo
An essential modulator of cell growth and division is the evolutionarily conserved kinase (S6K1). It is triggered by certain stimulants, including amino acids, insulin, and other growth hormones. The Akt/phosphatidylinositol 3-kinase pathwa...
Advantages and Limitations of AlphaFold in Structural Biology: Insights from Recent Studies [0.03%]
AlphaFold在结构生物学中的优势与局限性：近期研究的启示
Mandarina Qing Cheng Li,Sihan Wang,Shi-Ruei Lin et al.
Mandarina Qing Cheng Li et al.
Over the past three years, AlphaFold-a deep learning-based protein structure prediction system-has transformed structural biology by providing near-experimental accuracy models directly from amino acid sequences. This narrative review synth...
Peptide and Protein Tags for the Half-Life Extension of Therapeutic Agents: A Review Article [0.03%]
用于药物长效化的多肽和蛋白标签：综述文章
Sorour Aboutalebi,Fatemeh Najafi,Negin Yazdizadeh et al.
Sorour Aboutalebi et al.
Although biological drugs have been considered as one of the effective and growing therapeutic approaches in the pharmaceutical industry in recent decades, the largest concern about them is the insufficient stability and rapid degradation i...
Evaluating the Structural Stability of Recombinant Rotavirus Capsid Protein VP6 in Altered Physicochemical States Using Fluorescence and CD Spectroscopy [0.03%]
基于荧光和圆二色谱技术评价轮状病毒衣壳蛋白VP6重组体系在不同物化状态下结构稳定性
Modibedi Lesego Gladys,Preenan Pillay
Modibedi Lesego Gladys
Rotaviruses (RV) are a major cause of severe childhood diarrhoea, particularly in developing nations, necessitating stable vaccines. Therefore, the presented preliminary study aimed to assess the impact of altered physicochemical properties...
Non-ionic Detergent-Assisted Refolding of Protein from Protein-SDS Complex [0.03%]
蛋白从蛋白-十二烷基硫酸钠复合物的非离子去污剂辅助复性
Teruo Akuta,Tomoto Ura,Takeshi Oikawa et al.
Teruo Akuta et al.
Refolding of protein from denatured structure caused by sodium dodecyl sulfate (SDS) was examined using agarose native gel electrophoresis and circular dichroism (CD). Refolding of protein from SDS complex was induced with the addition of n...
Challenges in Serum Albumin Measurements: A Comparative Study of the Bromocresol Green and the Capillary Zone Electrophoretic Method [0.03%]
血清白蛋白测定的挑战：溴甲酚绿法与毛细管电泳法的比较研究
Mariem Othmani,Yessine Amri,Siwar Chelbi et al.
Mariem Othmani et al.
Serum albumin (SA) is a key biomarker routinely used in clinical practice. Multiple analytical methods exist for its measurement, including bromocresol green (BCG) colorimetry and capillary zone electrophoresis (CZE). However, discrepancies...
Arginine 11, Arginine 32, and Lysine 44 of a Staphylococcal Anti-sigma Factor are Indispensable Residues [0.03%]
金黄色葡萄球菌抗σ因子第11位的精氨酸、第32位的精氨酸和第44位的赖氨酸是不可或缺的残基
Debasmita Sinha,Debabrata Sinha,Tushar Chakraborty et al.
Debasmita Sinha et al.
σB, a Staphylococcus aureus-encoded alternative sigma factor, is inhibited by RsbW, an anti-sigma factor. RsbW also dimerizes in solution and phosphorylates RsbV, an RsbW antagonist. Of the predicted RsbW residues involved in its dimerizat...
Mahsa Cheshomi,Nikta Shobeiri,Amineh Sadat Tajani et al.
Mahsa Cheshomi et al.
Protein and peptide drugs have become essential in treating numerous diseases due to their high specificity and therapeutic potential. However, their clinical application faces several challenges, including chemical instability, physical in...
GCMS and Molecular Docking Based Insights into the Antifungal Potential of Bacillus Bs-06 Against Fusarium oxysporum f. sp. lycopersici [0.03%]
基于GCMS和分子对接的枯草芽孢杆菌Bs-06抗轮枝镰刀菌潜伏感染潜力研究新见解
K Vigneshwaran,K Rajamohan,P Balabaskar et al.
K Vigneshwaran et al.
The utilization of Bacillus spp. as eco-compatible bioresources represented a promising strategy in antifungal biotechnology. In this study, Bacillus subtilis Bs-06, isolated from the rhizosphere of healthy tomato plants, exhibited strong a...
Multi-scale In Silico and Biochemical Evaluation of Natural Bisbenzylisoquinoline Alkaloids as Aldose Reductase Inhibitors [0.03%]
天然双苯基异喹啉生物碱作为醛糖还原酶抑制剂的多尺度计算机及生化评价
Emadeldin M Kamel,Noha A Ahmed,Sarah I Othman et al.
Emadeldin M Kamel et al.
Aldose reductase (AR) is the rate-limiting enzyme of the polyol pathway and a validated target for preventing micro- and neurovascular complications of diabetes. Here, we combined multi-scale in-silico analyses with biochemical testing to e...