Thermal unfolding curves of high concentration bovine IgG measured by FTIR spectroscopy [0.03%]
傅里叶变换红外光谱法测定高浓度牛免疫球蛋白的热变性曲线
V Sathya Devi,Denis R Coleman,Jeremy Truntzer
V Sathya Devi
The purpose of this research is to study the thermal unfolding of high concentration bovine Immunoglobulin G (IgG) under 26 different experimental conditions by Fourier Transform Infrared spectroscopy with improved purge conditions and soft...
Effect of human serum albumin on the kinetics of 4-methylumbelliferyl-β-D-N-N'-N″ Triacetylchitotrioside hydrolysis catalyzed by hen egg white lysozyme [0.03%]
人血清白蛋白对鸡卵清溶菌酶催化4-甲基伞形酮-β-D-N-N'-N''三乙酰基巂氨三糖苷水解动力学的影响
Cristian Calderon,Elsa Abuin,Eduardo Lissi et al.
Cristian Calderon et al.
The effect of human serum albumin (HSA) addition on the rate of hydrolysis of the synthetic substrate 4-methylumbelliferyl-β-D-N-N'-N″ triacetylchitotrioside ((NAG)(3)-MUF) catalyzed by hen egg white lysozyme has been measured in aqueous ...
Leviserpin: a serine peptidase inhibitor (Serpin) from the Sugarcane Weevil Sphenophorus levis [0.03%]
Sphenophorus levis丝氨酸蛋白酶抑制剂levisermiン的克隆、表达及功能分析
Fernando P P Fonseca,Priscila T L Ike,Diego M Assis et al.
Fernando P P Fonseca et al.
Serine peptidase inhibitors (serpins) form a superfamily of proteins covering abroad spectrum of different biological functions. Here we describe the inhibitory characterization of leviserpin, the first serpin from the sugar cane weevil Sph...
Biochemical characterization of allantoinase from Escherichia coli BL21 [0.03%]
大肠杆菌Allantoin酶的生化性质研究
Ya-Yeh Ho,Hui-Chuan Hsieh,Cheng-Yang Huang
Ya-Yeh Ho
Bacterial allantoinase (ALLase; EC 3.5.2.5), which catalyzes the conversion of allantoin into allantoate, possesses a binuclear metal center in which two metal ions are bridged by a posttranslationally carboxylated lysine. Here, we characte...
Purification and characterization of a lectin with high hemagglutination property isolated from Allium altaicum [0.03%]
一种具有强凝血活性的葱属植物球茎蛋白的纯化及性质鉴定
Santosh Kumar Upadhyay,Sharad Saurabh,Rahul Singh et al.
Santosh Kumar Upadhyay et al.
A lectin was purified from the leaves of Allium altaicum and corresponding gene was cloned. The lectin namely Allium altaicum agglutinin (AAA) was ~24 kDa homodimeric protein and similar to a typical garlic leaf lectin. It was synthesized a...
Oxidative damage of lysozyme and human serum albumin and their mixtures: a comparison of photosensitized and peroxyl radical promoted processes [0.03%]
溶菌酶和人血清白蛋白及其混合物的氧化损伤:光敏与过氧自由基促进过程的比较研究
Andrea Arenas,Rodrigo Vasquez,Camilo López-Alarcón et al.
Andrea Arenas et al.
Oxidative modifications of lysozyme (Lyso) and human serum albumin (HSA) mediated by photoinduced processes and peroxyl radicals were studied. Both oxidative conditions were applied to the separate proteins and their mixtures. Dimerization ...
Comparative Study
The protein journal. 2011 Jun;30(5):359-65. DOI:10.1007/s10930-011-9341-1 2011
Heterologous expression of stress-responsive DUF538 domain containing protein and its morpho-biochemical consequences [0.03%]
异源表达逆境响应DUF538结构域蛋白及其形态生化效应
Ashraf Gholizadeh
Ashraf Gholizadeh
As a usual response, plants induce/activate various proteins which are thought to be involved in defense mechanisms against the biotic and abiotic stresses they may be confronted with. The novel DUF538 domain containing proteins with unknow...
Bactericidal activity identified in 2S Albumin from sesame seeds and in silico studies of structure-function relations [0.03%]
芝麻籽2S清蛋白具抗菌活性及结构功能关系的理论研究
Simone Maria-Neto,Rodrigo V Honorato,Fábio T Costa et al.
Simone Maria-Neto et al.
Pathogenic bacteria constitute an important cause of hospital-acquired infections. However, the misuse of available bactericidal agents has led to the appearance of antibiotic-resistant strains. Thus, efforts to seek new antimicrobials with...
Identification of a lysine residue important for the catalytic activity of yeast farnesyl diphosphate synthase [0.03%]
鉴定出一个对于酵母法尼基二磷酸合成酶催化活性非常重要的赖氨酸残基
Marc J C Fischer,Sophie Meyer,Patricia Claudel et al.
Marc J C Fischer et al.
The Saccharomyces cerevisiae ERG20 gene (encoding farnesyl diphosphate synthase) has been subjected to a set of mutations at the catalytic site, at position K254 to determine the in vivo impact. The mutated strains have been shown to exhibi...
Thermal denaturation of β-glucosidase B from Paenibacillus polymyxa proceeds through a Lumry-Eyring mechanism [0.03%]
多黏类芽孢杆菌β-葡萄糖苷酶B的热变性通过Lumry-Eyring机制进行
Menandro Camarillo-Cadena,Georgina Garza-Ramos,Mariana Peimbert et al.
Menandro Camarillo-Cadena et al.
β-glucosidase B (BglB), 1,4-β-D: -glucanohydrolase, is an enzyme with various technological applications for which some thermostable mutants have been obtained. Because BglB denatures irreversibly with heating, the stabilities of these mu...