Signal transduction of fertilization in frog eggs and anti-apoptotic mechanism in human cancer cells: common and specific functions of membrane microdomains [0.03%]
蛙卵受精信号转导和人类癌细胞抗凋亡机制中的膜微域的异同点研究
Ken-Ichi Sato
Ken-Ichi Sato
Membrane microdomains or lipid/membrane rafts are distinct areas on the plasma membranes, where a specific subset of lipids (e.g. cholesterol, sphingolipids) and proteins (e.g. glycosylphosphatidylinositol-anchored proteins, growth factor r...
Phytochemical approach and bioanalytical strategy to develop chaperone-based medications [0.03%]
植物化学方法及生物分析策略开发基于分子伴侣的药物
Kastenholz Bernd
Kastenholz Bernd
Currently, no pharmaceuticals for the etiological treatment of degenerative protein-misfolding diseases (e.g., ALS, Alzheimer's or prion diseases) are commercially available. In this technical note theoretical considerations and practical a...
Display of E. coli Alkaline Phosphatase pIII or pVIII Fusions on Phagemid Surfaces Reveals Monovalent Decoration with Active Molecules [0.03%]
利用噬菌粒表面展示系统实现E.coli碱性磷酸酶pIII或pVIII融合蛋白的单分子活性展示
Michael Weichel,Rolf Jaussi,Claudio Rhyner et al.
Michael Weichel et al.
Active alkaline phosphatase of Escherichia coli (PhoA, EC 3.1.3.1) was displayed via the leucine zipper element of the Jun-Fos heterodimer on the surface of filamentous phage and the kinetic parameters K(m) and k(cat) were determined. The p...
Screening Outside the Catalytic Site: Inhibition of Macromolecular Inter-actions Through Structure-Based Virtual Ligand Screening Experiments [0.03%]
基于结构的虚拟配体筛选实验在催化位点之外的筛查：通过抑制大分子相互作用实现
Olivier Sperandio,Maria A Miteva,Kenneth Segers et al.
Olivier Sperandio et al.
During these last 15 years, drug discovery strategies have essentially focused on identifying small molecules able to inhibit catalytic sites. However, other mechanisms could be targeted. Protein-protein interactions play crucial roles in a...
F Javier Moreno,Alfonso Clemente
F Javier Moreno
2S albumin storage proteins are becoming of increasing interest in nutritional and clinical studies as they have been reported as major food allergens in seeds of many mono- and di-cotyledonous plants. This review describes the main biochem...
Heparin alters viral serpin, serp-1, anti-thrombolytic activity to anti-thrombotic activity [0.03%]
肝素改变病毒丝氨酸蛋白酶抑制剂serp-1的抗纤溶活性为抗凝血活性
Xing Li,Heather Schneider,Andrew Peters et al.
Xing Li et al.
Serine protease inhibitors (serpins) regulate coagulation and inflammation. Heparin, a glycosaminoglycan, is an important cofactor for modulation of the inhibitory function of mammalian serpins. The secreted myxoma viral serpin, Serp-1 exer...
Consensus prediction of protein conformational disorder from amino acidic sequence [0.03%]
基于氨基酸序列的蛋白质构象无序区域共识预测方法
Suresh Kumar,Oliviero Carugo
Suresh Kumar
Predictions of protein conformational disorder are important in structural biology since they can allow the elimination of protein constructs, the three-dimensional structure of which cannot be determined since they are natively unfolded. H...
Angela de Laurentiis,Lorna Donovan,Alexandre Arcaro
Angela de Laurentiis
Lipid rafts and caveolae are microdomains of the plasma membrane enriched in sphingolipids and cholesterol, and hence are less fluid than the remainder of the membrane. Caveolae have an invaginated structure, while lipid rafts are flat regi...
Gillian D Pullinger,Alistair J Lax
Gillian D Pullinger
We have investigated histidine residues near the active site of the mitogenic Pasteurella multocida toxin. Mutation of H1202 or H1228 had little effect, while the effect of mutation on H1223 depended on the amino acid substituted. Mutation ...
Increased endogenous nitric oxide release by iron chelation and purinergic activation in the rat carotid body [0.03%]
铁螯合和嘌呤能激活增加大鼠颈动脉体的内源性一氧化氮释放量
Man-Lung Fung,Meifang Li,Sukhamay Lahiri
Man-Lung Fung
We examined the hypothesis that hypoxic chemotransduction with stabilization of HIF-1 and activation of purinoceptors stimulate the endogenous NO production in the rat carotid body. The effects of blockade of purinoceptors with suramin, or ...