Antioxidant properties of dimethyl sulfoxide and its viability as a solvent in the evaluation of neuroprotective antioxidants [0.03%]
二甲基亚砜的抗氧化性质及其作为溶剂评估神经保护性抗氧化剂的适用性
Carolina Sanmartín-Suárez,Ramón Soto-Otero,Inés Sánchez-Sellero et al.
Carolina Sanmartín-Suárez et al.
Introduction: Dimethyl sulfoxide is an amphiphilic compound whose miscibility with water and its ability to dissolve lipophilic compounds make it an appreciated solvent in biomedical research. However, its reported antiox...
Utility of quantitative whole-body autoradiography (QWBA) and imaging mass spectrometry (IMS) by matrix-assisted laser desorption/ionization (MALDI) in the assessment of ocular distribution of drugs [0.03%]
定量全身自辐射照相(QWBA)和基质辅助激光解析电离成像质谱(MALDI-IMS)技术在评估药物眼内分布中的应用价值
Dieter M Drexler,Sarah H Tannehill-Gregg,Lifei Wang et al.
Dieter M Drexler et al.
Introduction: Assessment of drug candidate properties and potential liabilities can greatly benefit from issue driven studies that are designed to address specific toxicological effects such as ocular phototoxicity. If a ...
Mohammed T Al Samri,Mariam Al Shamsi,Suhail Al-Salam et al.
Mohammed T Al Samri et al.
Introduction: A novel in vitro system was developed to measure O₂ consumption by murine tissues over several hours. Methods: Tissue sp...
Morten Laursen,Søren-Peter Olesen,Morten Grunnet et al.
Morten Laursen et al.
Introduction: The minipig represents an attractive experimental animal within cardiovascular research due to its extensive similarities to the human heart in terms of anatomy and physiology. Although minipigs have been us...
A multi-species assay for siRNA-mediated mRNA knockdown analysis without the need for RNA purification [0.03%]
无需纯化RNA即可进行siRNA介导的mRNA敲低分析的多物种检测试验
Zhibin Wang,Stephen J Pacchione,Zhutian Niu et al.
Zhibin Wang et al.
Introduction: Various animal models are routinely used to evaluate the efficacy and toxicity of small interfering RNA (siRNA) therapeutics. Given that the most common measure of efficacy with siRNA therapeutics is mRNA kn...
Effect of biological matrix and sample preparation on qPCR quantitation of siRNA drugs in animal tissues [0.03%]
生物基质及样品预处理对动物组织样本中siRNA药物qPCR定量分析的影响研究
Jessica Seitzer,Hangchun Zhang,Martin Koser et al.
Jessica Seitzer et al.
Introduction: Quantitative pharmacokinetic measurement of short nucleotide sequences in animal tissues is critical to the successful development of siRNA-based drugs. Stem-loop qRT-PCR is a sensitive and precise methodolo...
CMV promoter is inadequate for expression of mutant human RyR2 in transgenic rabbits [0.03%]
CMV启动子不足以在转基因兔中表达突变的人类RyR2蛋白质
Paulina Wakula,Egbert Bisping,Jens Kockskämper et al.
Paulina Wakula et al.
Introduction: Fundamental differences in Ca²+ homeostasis between mice and larger mammals require the validation of the mechanisms of arrhythmogenesis before translation into human pathophysiology. The purpose of this st...
Current approaches and issues in non-clinical evaluation of abuse and dependence [0.03%]
当前非临床评价药物滥用和依赖性的方法及存在问题
Paul Moser,Toni Wolinsky,Vincent Castagné et al.
Paul Moser et al.
Preclinical assessment of drug abuse and dependence has been the subject of several recent regulatory guidelines. Both the European and US authorities recommend a tiered approach and are generally aligned on the methods which should be used...
Comparison of the NIDS® rapid assay with ELISA methods in immunogenicity testing of two biotherapeutics [0.03%]
NIDS® 快速检测试剂与两种生物制剂免疫原性ELISA检测方法的比较评估
Jing Pan,Thomas Small,Dujie Qin et al.
Jing Pan et al.
Introduction: Rapid lateral flow immunogenicity assays for the detection of anti-drug antibodies (ADAs) to two biotherapeutic antibodies, an anti-HER2 antibody and an anti-TNF-α antibody, were developed using ANP Technol...
Validation of a cell-based assay to differentiate between the cytotoxic effects of elapid snake venoms [0.03%]
一种细胞毒性试验用于鉴定眼镜蛇毒和海蛇毒的差异性有效性研究
Yasmean Kalam,Geoffrey K Isbister,Peter Mirtschin et al.
Yasmean Kalam et al.
Introduction: Acanthophis genus (i.e. death adders) and the Naja genus (i.e. cobras) belong to the family elapidae. The current study compared the in vitro cytotoxicity of venoms from four Acanthophis spp. and three Naja ...