Impulsivity and drugs of abuse: a juice-reinforced operant procedure for determining within-session delay discounting functions in rhesus monkeys [0.03%]
一种确定食蟹猴限时剥夺作用功能的冲动性实验方法
David R Maguire,Jun-Xu Li,Charles P France
David R Maguire
Introduction: Impulsivity is a behavioral trait that is thought to contribute to a variety of disorders, including drug abuse. Efficient, sensitive procedures are needed for studying drug effects on impulsivity (e.g., del...
High throughput measurement of Ca²⁺ dynamics for drug risk assessment in human stem cell-derived cardiomyocytes by kinetic image cytometry [0.03%]
基于人类诱导多能干细胞分化的高通量药物致心律失常风险评估技术
Fabio Cerignoli,David Charlot,Ross Whittaker et al.
Fabio Cerignoli et al.
Current methods to measure physiological properties of cardiomyocytes and predict fatal arrhythmias that can cause sudden death, such as Torsade de Pointes, lack either the automation and throughput needed for early-stage drug discovery and...
Epigenetic histone acetylation and deacetylation mechanisms in experimental models of neurodegenerative disorders [0.03%]
神经退行性疾病模型中的表观遗传学组蛋白乙酰化和脱乙酰化机制
Zacharoula Konsoula,Frank A Barile
Zacharoula Konsoula
Introduction: Epigenetic modifications, such as histone acetylation and deacetylation, are responsible for maintaining chromatin stability. As such, they have been implicated in a wide range of neurodegenerative disorders...
Optimization of perfusate pH to improve microdialysis recovery of lipophilic compounds [0.03%]
优化灌注液pH以改进脂质化合物的微透析回收率
Emmanuelle S Tre,Chanchal Patel,Shiv Aghara et al.
Emmanuelle S Tre et al.
Introduction: Microdialysis (MD) allows sampling of compounds in-vivo from tissues' interstitial fluid. However, molecules insoluble at physiological pH have usually extremely low recovery. The addition of albumin to the ...
A method for rapid dose-response screening of environmental chemicals using human embryonic stem cells [0.03%]
一种使用人类胚胎干细胞快速筛查环境化学物质剂量效应关系的方法
R Z Behar,V Bahl,Y Wang et al.
R Z Behar et al.
Introduction: Human embryonic stem cells (hESC) provide an invaluable model for assessing the effects of environmental chemicals and drugs on human prenatal development. However, hESC are difficult to adapt to 96-well pla...
Drug-induced effects on cardiovascular function in pentobarbital anesthetized guinea-pigs: invasive LVP measurements versus the QA interval [0.03%]
戊巴比妥麻醉下苯二氮卓类药物对豚鼠心血管功能的影响：导管留置直接左室压测定与球囊消融间期法的比较
D M Johnson,R Geys,J Lissens et al.
D M Johnson et al.
Introduction: Evaluation of drug-related effects on cardiovascular function is part of the core battery described in the ICH S7A guideline. Anesthetized guinea-pigs are excellent models for the evaluation of drug-induced ...
An 'all-inclusive' 96-well cytochrome P450 induction method: measuring enzyme activity, mRNA levels, protein levels, and cytotoxicity from one well using cryopreserved human hepatocytes [0.03%]
一种“一站式”的96孔细胞色素P450诱导方法：利用冷冻保存的人肝细胞从一个孔中测量酶活性、mRNA水平、蛋白水平和细胞毒性
Jason S Halladay,Susan Wong,S Cyrus Khojasteh et al.
Jason S Halladay et al.
Introduction: The traditional in vitro approach for assessing potential CYP induction has been to simply compare changes in CYP activities using known CYP-specific probe substrates following exposure to the test compound ...
An inexpensive system for evaluating the tussive and anti-tussive properties of chemicals in conscious, unrestrained guinea pigs [0.03%]
一种在清醒且未受限制的 Guinea 猪中评估化学品祛痰和镇咳性质的廉价系统
J R Daller,J Wong,B D Brooks et al.
J R Daller et al.
Introduction: Commercial whole-body plethysmography systems used to evaluate the anti-tussive potential of drugs employ sophisticated technology, but these systems may be cost prohibitive for some laboratories. The presen...
The comparative sensitivity of three in vitro safety pharmacology models for the detection of lidocaine-induced cardiac effects [0.03%]
三种体外安全药理模型检测利多卡因诱导的心脏效应的敏感性比较
Sonia Goineau,Vincent Castagné,Philippe Guillaume et al.
Sonia Goineau et al.
Introduction: In the current ICH S7B guideline, in vitro evaluation of proarrhythmic liability is limited to the risk of QT interval prolongation, whilst the effect of new chemical entities on cardiac conductivity is ofte...
Left ventricular pressure, contractility and dP/dt(max) in nonclinical drug safety assessment studies [0.03%]
非临床药物安全性评价研究中的左心室压力、收缩力和dP/dt(max)参数
R Dustan Sarazan,John P Kroehle,Bradley W Main
R Dustan Sarazan
Increasing or decreasing cardiac contractility is an undesirable property of drugs being developed for noncardiovascular indications. The International Conference on Harmonization (ICH) Topic S7A and S7B guidelines only require the assessme...