Pharmacokinetic profiles of methylcobalamin in rats after multiple administration routes by a simple LC-MS/MS assay with a small volume of plasma [0.03%]
一种简单LC-MS/MS小血浆量测定方法用于评价不同给药途径甲基钴胺在大鼠体内的药代动力学特性
Koichiro Hotta,Yuji Mano
Koichiro Hotta
Methylcobalamin (MBL) is a vitamin B12 coenzyme and is effective for treating peripheral neuropathies. Little is known about pharmacokinetics (PK) of MBL in animals, we have developed a simple assay for MBL by using only 0.01 mL of plasma f...
The efficient method to get better raw brain signal on rat anesthetics experiment [0.03%]
大鼠麻醉实验下获取更好原始脑信号的高效方法
Ye Yuan,Sinan Li,Linyan Wu et al.
Ye Yuan et al.
This paper introduces an efficient methodology for conducting rat anesthesia experiments, aimed at enhancing the quality of raw brain signals obtained. The proposed approach enables the acquisition of animal brain signals during experiments...
Comparative analysis of high-throughput RNA extraction kits in Naïve Non-Human Primate (NHP) tissues for downstream applications utilizing Xeno Internal Positive Control (IPC) [0.03%]
含外来阳性内控(Xeno IPC)的高通量RNA提取试剂盒在非人灵长类动物(NHP)组织中的下游应用的比较分析
Ruwini D Rajapaksha,Catherine Brooks,Adriana Rascon et al.
Ruwini D Rajapaksha et al.
Ribonucleic acid (RNA) extraction and purification play pivotal roles in molecular biology and cell and gene therapy, where the quality and integrity of RNA are critical for downstream applications. Automated high-throughput systems have ga...
Understanding lymphatic drug delivery through chylomicron blockade: A retrospective and prospective analysis [0.03%]
通过乳糜微粒阻塞理解淋巴药物输送：回顾性和前瞻性分析
Malaz Yousef,Nadia Bou-Chacra,Raimar Löbenberg et al.
Malaz Yousef et al.
Scientists have developed and employed various models to investigate intestinal lymphatic uptake. One approach involves using specific blocking agents to influence the chylomicron-mediated lymphatic absorption of drugs. Currently utilized m...
Detection of contractility changes in the heart from arterial blood pressure data using symmetric Projection Attractor Reconstruction [0.03%]
基于动脉血压数据对心脏收缩力变化的对称投影吸引子重构检测法
Esther Bonet-Luz,Manasi Nandi,Mark I Christie et al.
Esther Bonet-Luz et al.
The potential for unintended drug induced changes in cardiac contractility is a major concern in medicines development. Whilst direct left ventricular pressure (LVP) measurement is the gold standard for measuring cardiac contractility in vi...
Development and validation of an LC-MSMS method for the quantitation of pacritinib; application of kinetics in rabbits [0.03%]
帕克利替尼定量的LC-MS-MS方法的发展与验证；兔子中的动力学应用研究
Phani Kumar Sunkara,Sreedhara Chaganty,K Ramakrishna
Phani Kumar Sunkara
Background: Accurate and selective LC/ESI-MSMS method development and validation for the quantitation of pacritinib is the primary goal of this study to perform kinetic studies in the healthy rabbit. ...
Preclinical mitigation of 5-HT2B agonism-related cardiac valvulopathy revisited [0.03%]
5-羟色胺2B受体激动剂相关心脏瓣膜病的临床前防治方法再探讨
Bérengère M Dumotier,Laszlo Urban
Bérengère M Dumotier
Cardiac valvulopathy (Cardiac Valve Disease; CVD) associated with off-target activation of the 5-hydroxytryptamine (5-HT) 2B receptor has been well recognized, but is still poorly predicted during drug development. The regulatory guidance p...
Assessment of corrected JT-peak (JTpc) and Tpeak-to-Tend (TpTec) as proarrhythmia biomarkers in non-human primates: Outcome from a HESI consortium [0.03%]
评估校正后的JT峰(JTpc)和T波峰间期(TpTec)在非人灵长类动物中作为致心律失常生物标志物的效果：HESI联盟的结果
Emmanuel Boulay,Simon Authier,Theresa Bartko et al.
Emmanuel Boulay et al.
Introduction: Corrected QT interval (QTc)is an established biomarker for drug-induced Torsade de Pointe (TdP), but with concerns for a false positive signal. Clinically, JTpc and TpTec have emerged as ECG sub-intervals to...
Nonclinical pharmacokinetics of E3112, a recombinant human hepatocyte growth factor, in rats and monkeys by a simple ELISA kit assay [0.03%]
一种新型人重组肝细胞生长因子E3112在大鼠和猴体内的非临床药代动力学研究（使用简易ELISA试剂盒）
Muneo Aoyama,Yuji Mano
Muneo Aoyama
E3112 is a recombinant human hepatocyte growth factor which is under development for the treatment of acute liver failure. Pharmacokinetics (PK) evaluation in experimental animals is important and thus a simple assay for the determination o...
Ultra-fast UPLC-MS/MS approach for estimating X-376 in human liver microsomes: Evaluation of metabolic stability via in silico software and in vitro analysis [0.03%]
一种超快速UPLC-MS/MS法测定人类肝微粒体中X-376的方法：通过计算机软件和体外实验评估其代谢稳定性
Mohamed W Attwa,Ali S Abdelhameed,Adnan A Kadi
Mohamed W Attwa
X-376 is a novel anaplastic lymphoma kinase (ALK) inhibitor that is capable of penetrating the blood brain barrier. This makes it suitable for use in patients with ALK-positive non-small cell lung cancer (NSCLC) who have metastases in the c...