Evaluating the proarrhythmic risk of delayed-action compounds in serum free cell culture conditions; serum-starvation accelerates/amplifies the effect of probucol on the KCNQ1 + KCNE1 channel [0.03%]
延迟起效药物在血清无细胞培养条件下致心律失常风险的评估；脱脂诱导probucol对KCNQ1+KCNE1电流作用加快/加强
Kenny M Van Theemsche,Lisse Frans,Dieter V Van de Sande et al.
Kenny M Van Theemsche et al.
In vitro testing procedures for evaluating acute effects of compound on ion channels, utilizing heterologous expression systems (HES), are well-established, while slowly manifesting delayed effects remain challenging to detect. For this, im...
Comparative toxicity assessment of selected nanoparticles using different experimental model organisms [0.03%]
选择性纳米颗粒的比较毒性评估的不同实验模型生物体中
Srishti Parashar,Sheetal Raj,Priyanka Srivastava et al.
Srishti Parashar et al.
Nanoparticles are microscopic particles ranging in size from one to one hundred nanometers. Due to their extensive features, nanoparticles find widespread use in various fields worldwide, including cosmetics, medical diagnosis, pharmaceutic...
Inhibition of hERG K channels by verapamil at physiological temperature: Implications for the CiPA initiative [0.03%]
维拉帕米在生理温度下对hERG钾通道的抑制作用以及其对CiPA计划的意义
Ashley A Johnson,Matthew C Trudeau
Ashley A Johnson
The Comprehensive in vitro Proarrhythmia Assay (CiPA) initiative reassesses using the inhibition of hERG potassium channels by drugs as the major determinant for the potential to cause drug-induced Torsades de Pointes (TdP) cardiac arrhythm...
Assessment of antiemetic activity of dihydrocoumarin: In vivo and in silico approaches on receptor binding affinity and modulatory effects [0.03%]
评估二氢香豆素的止吐活性：受体结合亲和力及调节作用的体内与体外研究
Md Abu Saim,Md Shimul Bhuia,Tanzila Akter Eity et al.
Md Abu Saim et al.
Dihydrocoumarin (DCN) is a natural compound widely used in the flavor industry and has antioxidant and anti-inflammatory properties. However, its potential antiemetic effects on gastrointestinal disturbances remain untested. This study emph...
Overcoming obstacles in three-dimensional cell culture model establishment: Approaches for growing A549 non-small cell lung cancer spheroids using a clinostat system [0.03%]
克服三维细胞培养模型建立中的障碍：使用临床定位仪系统培养A549非小细胞肺癌类球体的方法
Charity M Mabela,Chrisna Gouws,Wihan Pheiffer
Charity M Mabela
Introduction: Non-small cell lung cancer (NSCLC) accounts for 80-85 % of lung cancer cases globally. And the A549 cell line is widely used in pharmacological and toxicity screening. Due to its popularity as a NSCLC model,...
A general and rapid LC-MS/MS method for simultaneous determination of voriconazole, posaconazole, fluconazole, itraconazole and hydroxyitraconazole in IFI patients [0.03%]
一种用于侵袭性曲霉病患者体内伏立康唑、泊沙康唑、氟康唑、伊曲康唑和羟基伊曲康唑的同时测定的通用快速LC-MS/MS方法
Wenwen Xia,Shun Chen,Yunlei Yun et al.
Wenwen Xia et al.
Objective: To establish a rapid and universal quantitative liquid chromatography tandem mass spectrometry (LC-MS/MS) method for measuring the exposure levels of five triazole antifungal drugs in human plasma, including vo...
Pharmacokinetic profiles of methylcobalamin in rats after multiple administration routes by a simple LC-MS/MS assay with a small volume of plasma [0.03%]
一种简单LC-MS/MS小血浆量测定方法用于评价不同给药途径甲基钴胺在大鼠体内的药代动力学特性
Koichiro Hotta,Yuji Mano
Koichiro Hotta
Methylcobalamin (MBL) is a vitamin B12 coenzyme and is effective for treating peripheral neuropathies. Little is known about pharmacokinetics (PK) of MBL in animals, we have developed a simple assay for MBL by using only 0.01 mL of plasma f...
The efficient method to get better raw brain signal on rat anesthetics experiment [0.03%]
大鼠麻醉实验下获取更好原始脑信号的高效方法
Ye Yuan,Sinan Li,Linyan Wu et al.
Ye Yuan et al.
This paper introduces an efficient methodology for conducting rat anesthesia experiments, aimed at enhancing the quality of raw brain signals obtained. The proposed approach enables the acquisition of animal brain signals during experiments...
Comparative analysis of high-throughput RNA extraction kits in Naïve Non-Human Primate (NHP) tissues for downstream applications utilizing Xeno Internal Positive Control (IPC) [0.03%]
含外来阳性内控(Xeno IPC)的高通量RNA提取试剂盒在非人灵长类动物(NHP)组织中的下游应用的比较分析
Ruwini D Rajapaksha,Catherine Brooks,Adriana Rascon et al.
Ruwini D Rajapaksha et al.
Ribonucleic acid (RNA) extraction and purification play pivotal roles in molecular biology and cell and gene therapy, where the quality and integrity of RNA are critical for downstream applications. Automated high-throughput systems have ga...
Understanding lymphatic drug delivery through chylomicron blockade: A retrospective and prospective analysis [0.03%]
通过乳糜微粒阻塞理解淋巴药物输送：回顾性和前瞻性分析
Malaz Yousef,Nadia Bou-Chacra,Raimar Löbenberg et al.
Malaz Yousef et al.
Scientists have developed and employed various models to investigate intestinal lymphatic uptake. One approach involves using specific blocking agents to influence the chylomicron-mediated lymphatic absorption of drugs. Currently utilized m...