Improved translation of Nav1.5 channel inhibition to in vivo QRS interval prolongation via the hIPSC cardiomyocyte model [0.03%]
人诱导多能干细胞心肌细胞模型改善Nav1.5通道抑制的临床前评价效能
John P Imredy,Holly Clouse,Mei Zhang et al.
John P Imredy et al.
Introduction: Drug risk assessment to ventricular conduction typically involves measuring functional inhibition of the cardiac sodium channel (Nav1.5) followed by nonclinical in vivo assessment of prolongation of the elec...
Metabolomics using high-resolution magic angle spinning nuclear magnetic resonance: Neurotoxicological applications [0.03%]
基于高分辨率魔角旋转核磁共振的代谢组学在神经毒理学中的应用
Rachel Neita,Kenna Hynes,Grace V Mercer et al.
Rachel Neita et al.
High-resolution magic angle spinning nuclear magnetic resonance (HRMAS NMR) is a cutting-edge technology for metabolomics studies that has found application in pharmacology and toxicology. Metabolomics provides insight into how biological s...
Thermal shift assays in drug discovery - Troubleshooting from biochemical to cellular applications [0.03%]
药物发现中的热移位测定法-从生化应用到细胞应用的故障排除
Danielle Hanke,Jennifer I Brown,Brent D G Page
Danielle Hanke
Thermal shift assays (TSAs) have become valuable tools within drug discovery and development pipelines. These techniques detect direct physical interactions between small, drug-like molecules and their target proteins in biochemical and cel...
Anticoagulant activity of Eucalyptus essential oils: An in vitro approach and a bioinformatics-based pharmacokinetic-pharmacodynamic analysis [0.03%]
基于生物信息学的药代-药效分析及尤加利精油的体外抗凝血活性研究
Jhonatan Felipe Dos Santos,Maria Eduarda Dias Possate,Maysa Barbosa de Almeida et al.
Jhonatan Felipe Dos Santos et al.
Coagulation disorders pose significant health risks, requiring effective therapies. Natural products emerge as promising anticoagulant agents, standing out in the search for innovative and safe alternatives, integrating in vitro studies, ph...
Standardizing high-throughput RNA extraction: Modifying commercial kits for improved purity, yield, and efficiency [0.03%]
高通量RNA提取标准化：改良商业试剂盒以提高纯度、产量和效率
Ruwini D Rajapaksha,Catherine Brooks,Adriana Rascon et al.
Ruwini D Rajapaksha et al.
High-throughput extraction kits are widely use in clinical and preclinical settings to extract nucleic acids from biological samples due to their scalability, repeatability, and reduced labor. However, achieving high nucleic acid yield and ...
From cellular waste to biomarkers; insights into past, present, and future methods to detect immune cell-derived extracellular vesicles using flow cytometry [0.03%]
从细胞废物到生物标志物；使用流式细胞术检测免疫细胞源性胞外囊泡的过去、现在和未来的方法见解
Jennifer L Zagrodnik,Craig S Moore
Jennifer L Zagrodnik
Extracellular vesicles (EVs) often possess both ubiquitous and unique tetraspanin molecules that can help elucidate their cell-of-origin. Furthermore, the presence and/or absence of specific tetraspanins can be used to phenotype and identif...
Toxicology and safety pharmacology investigations on the nervous system: 2024 industry survey [0.03%]
2024年神经系统的毒理学和安全药理学调查研究报告
Simon Authier,Marcus S Delatte,Alison Wakeford et al.
Simon Authier et al.
The American College of Toxicology (ACT), the Safety Pharmacology Society (SPS) and the Society for Toxicological Pathology (STP) conducted an industry survey in 2024 to assess current industry practices as they relate to neurotoxicity and ...
Current practices on the measurement of electrocardiogram and hemodynamic parameters in non-rodent species in regulatory safety assessment studies [0.03%]
非小鼠类动物电生理和血流动力学参数的测量在监管安全评估中的应用现状
Jean-Pierre Valentin,Todd Bourcier,Xuan Chi et al.
Jean-Pierre Valentin et al.
Introduction: Cardiovascular (CV) parameters1 such as blood pressure (BP), electrocardiogram (ECG), and heart rate (HR) are recorded in non-rodent non-clinical safety studies to support drug development. However, measurem...
Characterization of a human wearable ECG device for continuous data collection in freely moving non-rodents [0.03%]
一种用于自由活动非啮齿类动物穿戴式心电图装置的特性分析方法
Christopher P Regan,Alysia A Chaves,Theodore Detwiler et al.
Christopher P Regan et al.
Wearable devices are commonly used in clinical diagnostic medicine/personal health, but their use to collect nonclinical endpoints is limited due species-specific design specifications, data accessibility limitations, and availability of no...
Implementation of automated blood sampling in a canine cardiovascular safety pharmacology study to support PK/PD assessment [0.03%]
用于支持药代动力学/药效学评估的犬心血管安全药理学研究中自动采血系统的应用实施
Elham Ataei Alizadeh,Georg Rast,Jessica Schiwon et al.
Elham Ataei Alizadeh et al.
Introduction: Pharmacokinetic (PK) and pharmacodynamic (PD) modeling can elucidate the temporal interplay between drug concentration and physiological effects. Its use in safety pharmacology studies has been hindered, sin...