Standardizing high-throughput RNA extraction: Modifying commercial kits for improved purity, yield, and efficiency [0.03%]
高通量RNA提取标准化：改良商业试剂盒以提高纯度、产量和效率
Ruwini D Rajapaksha,Catherine Brooks,Adriana Rascon et al.
Ruwini D Rajapaksha et al.
High-throughput extraction kits are widely use in clinical and preclinical settings to extract nucleic acids from biological samples due to their scalability, repeatability, and reduced labor. However, achieving high nucleic acid yield and ...
From cellular waste to biomarkers; insights into past, present, and future methods to detect immune cell-derived extracellular vesicles using flow cytometry [0.03%]
从细胞废物到生物标志物；使用流式细胞术检测免疫细胞源性胞外囊泡的过去、现在和未来的方法见解
Jennifer L Zagrodnik,Craig S Moore
Jennifer L Zagrodnik
Extracellular vesicles (EVs) often possess both ubiquitous and unique tetraspanin molecules that can help elucidate their cell-of-origin. Furthermore, the presence and/or absence of specific tetraspanins can be used to phenotype and identif...
Toxicology and safety pharmacology investigations on the nervous system: 2024 industry survey [0.03%]
2024年神经系统的毒理学和安全药理学调查研究报告
Simon Authier,Marcus S Delatte,Alison Wakeford et al.
Simon Authier et al.
The American College of Toxicology (ACT), the Safety Pharmacology Society (SPS) and the Society for Toxicological Pathology (STP) conducted an industry survey in 2024 to assess current industry practices as they relate to neurotoxicity and ...
Current practices on the measurement of electrocardiogram and hemodynamic parameters in non-rodent species in regulatory safety assessment studies [0.03%]
非小鼠类动物电生理和血流动力学参数的测量在监管安全评估中的应用现状
Jean-Pierre Valentin,Todd Bourcier,Xuan Chi et al.
Jean-Pierre Valentin et al.
Introduction: Cardiovascular (CV) parameters1 such as blood pressure (BP), electrocardiogram (ECG), and heart rate (HR) are recorded in non-rodent non-clinical safety studies to support drug development. However, measurem...
Characterization of a human wearable ECG device for continuous data collection in freely moving non-rodents [0.03%]
一种用于自由活动非啮齿类动物穿戴式心电图装置的特性分析方法
Christopher P Regan,Alysia A Chaves,Theodore Detwiler et al.
Christopher P Regan et al.
Wearable devices are commonly used in clinical diagnostic medicine/personal health, but their use to collect nonclinical endpoints is limited due species-specific design specifications, data accessibility limitations, and availability of no...
Implementation of automated blood sampling in a canine cardiovascular safety pharmacology study to support PK/PD assessment [0.03%]
用于支持药代动力学/药效学评估的犬心血管安全药理学研究中自动采血系统的应用实施
Elham Ataei Alizadeh,Georg Rast,Jessica Schiwon et al.
Elham Ataei Alizadeh et al.
Introduction: Pharmacokinetic (PK) and pharmacodynamic (PD) modeling can elucidate the temporal interplay between drug concentration and physiological effects. Its use in safety pharmacology studies has been hindered, sin...
Results from an EFPIA, JPMA, PhRMA industry survey on reopening and revamping the ICH S7A guidance on safety and secondary pharmacology [0.03%]
EFPIA、JPMA和PhRMA关于恢复和重组ICH S7A指南（安全性和继发药理学）的行业调查结果
Jean-Pierre Valentin,Katsuyoshi Chiba,Derek Leishman et al.
Jean-Pierre Valentin et al.
The ICH S7A guideline on safety pharmacology has remained unchanged since its inception in 2000, fulfilling its crucial role in safeguarding clinical trial participants and patients (ICH S7A). However, in the meanwhile there has been signif...
Development and validation of a quantitative UHPLC-HRMS bioanalytical method for equine anti-doping control [0.03%]
赛马反兴奋剂控制的定量UHPLC-HRMS生化分析方法的开发和验证
Wafaa El-Ghaly,Taha El Kamli,Ange Melisse Ariane Gongbe et al.
Wafaa El-Ghaly et al.
The quantification of banned substances in equine antidoping control, especially in racehorse urine, necessitates robust analytical methods with high detection levels due to the extremely low concentrations of the target substances and the ...
Telemetry in toxicology studies - A comparison of data across species, technologies and time [0.03%]
毒理学研究中的遥测技术——跨物种、技术和时间的比较数据
Derek D Best,Matthew M Abernathy,Derek J Leishman
Derek D Best
Telemetry technology allows collection of quantitative cardiovascular safety pharmacology data in repeat dose toxicology studies. The ICH M3(R2) guideline suggests that inclusion of safety pharmacology endpoints into toxicology studies shou...
High-content cell imaging for chemical toxicity screening in the model organism Caenorhabditis elegans [0.03%]
秀丽线虫高内涵细胞成像化学毒性筛选体系研究
Camiryn J Kardal,Spencer R Dmytruk,Abdullah Qureshi et al.
Camiryn J Kardal et al.
The use of animal models for screening environmental chemicals for toxicity is an important step towards determining potential hazards to humans. Due to the large number of environmental chemicals with unknown biological activity, high-thro...