Reliable identification of cardiac liability in drug discovery using automated patch clamp: Benchmarking best practices and calibration standards for improved proarrhythmic assessment [0.03%]
使用自动化膜片钳技术可靠地识别药物研发中的心脏缺陷：改进致心律失常评估的最佳实践和校准标准的基准测试
Nina Brinkwirth,Kiyoshi Takasuna,Masafumi Doi et al.
Nina Brinkwirth et al.
Introduction: Screening compounds for activity on the hERG channel using patch clamp is a crucial part of safety testing. Automated patch clamp (APC) is becoming widely accepted as an alternative to manual patch clamp in ...
An improved two-step method for extraction and purification of primary cardiomyocytes from neonatal mice [0.03%]
一种改进的分离培养小鼠原代心脏细胞的方法
Chengbao Li,Xuli Rong,Jing Qin et al.
Chengbao Li et al.
Introduction: Primary mouse cardiomyocytes are essential tools for cardiovascular pharmacology research at the cellular and molecular levels, but their low viability and low purity have often caused challenges in previous...
Intrapulmonary inoculation of multicellular spheroids to construct an orthotopic lung cancer xenograft model that mimics four clinical stages of non-small cell lung cancer [0.03%]
用于构建模拟四期非小细胞肺癌临床阶段的原位移植瘤模型的多细胞球形体的肺内注射
Yingbo Huang,Yifan Lu,Mallika Vadlamudi et al.
Yingbo Huang et al.
Introduction: Lung cancer leads in mortality among all types of cancer in US and Non-small cell lung cancer (NSCLC) is the major type of lung cancer. Mice models of lung cancer based on subcutaneous or orthotopic inoculat...
Using a qPCR device to screen for modulators of ABC transporter activity: A step-by-step protocol [0.03%]
使用qPCR装置筛选ABC转运蛋白活性调节物的逐步协议
Luisa Möhle,Barbora Schwarzová,Markus Krohn et al.
Luisa Möhle et al.
Introduction: Adenosine triphosphate (ATP)-binding cassette (ABC) transporters are transmembrane proteins which actively transport a large variety of substrates across biological membranes. ABC transporter overexpression ...
The application of electrophysiological methods to characterize AMPA receptors in dissociated adult rat and non-human primate cerebellar neurons for use in neuronal safety pharmacology assessments of the central nervous system [0.03%]
电生理方法在分离成人大鼠和非人灵长类小脑神经元AMPA受体中的应用，用于中枢神经系统神经安全药理学评估
Loïs S Miraucourt,Michael V Accardi,Karen E Asin et al.
Loïs S Miraucourt et al.
Introduction: Pre-clinically, safety risk assessment of a drug is primarily tested in vivo using functional evaluation of adult animals while the mechanistic etiology of drug-induced CNS adverse effects is often uncharact...
Unraveling the mechanism and the risk behind seizure liability of lead compounds in a neuroscience project [0.03%]
揭秘神经科学项目中先导化合物致癫痫作用机制和风险研究
Annie Delaunois,Michael V Accardi,Véronique André et al.
Annie Delaunois et al.
Introduction: Several compounds from a neuroscience project induced convulsions in animals, at low exposure levels via a hypothetical off-target mechanism. A set of in vitro and in vivo experiments were conducted in order...
Combined cSLO-OCT imaging as a tool in preclinical ocular toxicity testing: A comparison to standard in-vivo and pathology methods [0.03%]
结合cSLO-OCT成像作为临床前眼部毒性检测的工具与常规活体和病理学方法比较的研究
Petr Soukup,Barbara Lenz,Bernd Altmann et al.
Petr Soukup et al.
Introduction: Confocal scanning laser ophthalmoscopy and optical coherence tomography (cSLO-OCT) became available for human and animal ophthalmic examinations in recent years. The purpose of this study was to evaluate les...
A capillary electrophoresis based approach for the identification of anti-drug antibodies against camelid VHH biologics (Nanobodies®) [0.03%]
一种用于鉴定骆驼类VHH生物制剂(Nanobodies®)抗药抗体的毛细管电泳方法
Derek Wiswell,Divas Neupane,Minchao Chen et al.
Derek Wiswell et al.
Undesired immune responses against protein therapeutics may adversely affect the pharmacokinetics, efficacy, and safety of the product. The presence of anti-drug-antibodies (ADA) has been the key determinant of immunogenic responses. Here w...
Drug-induced QT prolongation: Concordance of preclinical anesthetized canine model in relation to published clinical observations for ten CiPA drugs [0.03%]
CiPA十大药物在麻醉犬模型中的药代动力学研究及其与临床观测结果的一致性分析——用于预测心室复极延长的体外综合项目
Yevgeniya E Koshman,Amanda S Wilsey,Brandan M Bird et al.
Yevgeniya E Koshman et al.
Introduction: The Comprehensive In Vitro Proarrhythmia Assay (CiPA) initiative differentiates torsadogenic risk of 28 drugs affecting ventricular repolarization based on multiple in vitro human derived ionic currents. How...
Comprehensive analysis of cardiac function, blood biomarkers and histopathology for milrinone-induced cardiotoxicity in cynomolgus monkeys [0.03%]
用于恒河猴的米力农致心肌毒性的全面心脏功能、血液生物标志物和组织病理学分析
Katsuyoshi Chiba,Tomomichi Ishizaka,Yu Yoshimatsu et al.
Katsuyoshi Chiba et al.
The objective of this study was to elucidate the underlying cardiotoxic mechanism of milrinone, a cAMP phosphodiesterase 3 inhibitor, by evaluating cardiac functions, blood biomarkers including cardiac troponin I (cTnI), microRNAs (miR-1, m...