Nicholas D Ether,Sanket R Jantre,Dhruv B Sharma et al.
Nicholas D Ether et al.
The use of QT-prolongation as a biomarker for arrhythmia risk requires that researchers correct the QT-interval (QT) to control for the influence of heart rate (HR). QT correction methods can vary but most used are the universal correction ...
Prediction of GABA receptor antagonist-induced convulsion in cynomolgus monkeys by combining machine learning and heart rate variability analysis [0.03%]
通过结合机器学习和心率变异性分析预测恒河猴由GABA受体拮抗剂引起的抽搐
Shoya Nagata,Koichi Fujiwara,Kazuhiro Kuga et al.
Shoya Nagata et al.
Drug-induced convulsion is a severe adverse event; however, no useful biomarkers for it have been discovered. We propose a new method for predicting drug-induced convulsions in monkeys based on heart rate variability (HRV) and a machine lea...
Reliable identification of cardiac conduction abnormalities in drug discovery using automated patch clamp II: Best practices for Nav1.5 peak current in a high throughput screening environment [0.03%]
利用自动化膜片钳技术II：在高通量筛选环境中可靠地识别药物发现中的心脏传导异常的最佳实践——Nav1.5峰值电流
Maria Giustina Rotordam,Alison Obergrussberger,Nina Brinkwirth et al.
Maria Giustina Rotordam et al.
Introduction: For reliable identification of cardiac safety risk, compounds should be screened for activity on cardiac ion channels in addition to hERG, including NaV1.5 and CaV1.2. We identified different parameters that...
A calcium fluorescence assay for quantification of cholinergic receptor activity of clinical drugs in serum - comparison with radioactive methods [0.03%]
临床药物血清胆碱受体活性定量的钙荧光测定法与放射性方法比较研究
José N Nobrega,Roger Raymond,Tarek K Rajji et al.
José N Nobrega et al.
A new approach is described for quantifying cholinergic receptor activation status human blood samples, based on M1 receptor-driven mobilization of intracellular calcium stores. The assay identifies anticholinergic as well as agonist cholin...
Cytochrome P450 3A4 (CYP3A4) protein quantification using capillary western blot technology and total protein normalization [0.03%]
应用毛细管western blot技术和总蛋白归一化定量细胞色素P450 3A4(CYP3A4)蛋白质
Joseph M Collins,Danxin Wang
Joseph M Collins
The western blot (WB) is the predominate method for protein quantification, frequently used in pharmacological and toxicological studies. To control for technical variation, WB signals are normalized through immunodetection of an internal s...
Automated blood sampling in canine telemetry studies: Enabling enhanced assessments of cardiovascular liabilities and safety margins [0.03%]
犬类遥测研究中的自动化血液采样：提高心血管风险和安全边际评估的能力
Yevgeniya E Koshman,Amanda S Wilsey,Brandan M Bird et al.
Yevgeniya E Koshman et al.
Introduction: A successful integration of automated blood sampling (ABS) into the telemetry instrumented canine cardiovascular model is presented in this study. This combined model provides an efficient means to quickly g...
L-citrulline: A preclinical safety biomarker for the small intestine in rats and dogs in repeat dose toxicity studies [0.03%]
L-瓜氨酸：作为重复给药毒性试验中大鼠和犬小肠安全性的生物标志物的初步研究
Sven Jäckel,Frederic Christian Pipp,Barbara Emde et al.
Sven Jäckel et al.
Introduction: Gastrointestinal (GI) toxicity is still an issue within drug development, especially for novel oncology drugs. The identification of GI mucosal damage at an early stage with high sensitivity and specificity ...
Automated blood sampling in canine telemetry model: Enhanced assessment of immune liabilities [0.03%]
自动化犬类遥测模型血液采样：改进免疫缺陷评估方法
Yevgeniya E Koshman,Jie Lai-Zhang,Amanda S Wilsey et al.
Yevgeniya E Koshman et al.
Introduction: This manuscript presents a successful integration of multi-timepoint biomarker blood sampling (e.g., cytokines) in a conscious dog cardiovascular study using automated blood sampling via vascular access port...
Solvents effect on dansyl cysteamine depletion and reactivity classification of skin sensitizers: Tackling the challenges using binary solvent systems [0.03%]
溶剂对丹磺酰半胱胺耗尽及皮肤致敏物反应性分类的影响：使用二元溶剂系统应对挑战
Jongmin Ahn,Cristina Avonto,Amar G Chittiboyina et al.
Jongmin Ahn et al.
The high throughput method using dansyl cysteamine (HTS-DCYA™) is a sensitive and rapid in chemico approach to characterize skin sensitizers' thio-reactivity. The direct quantification of fluorescent hapten-DCYA adducts facilitates the rap...
Use of automated patch clamp in cardiac safety assessment: Past, present & future perspectives [0.03%]
自动化膜片钳技术在心脏安全性评价中的应用：过去、现在及未来展望
Damian C Bell,Bernard Fermini
Damian C Bell
There is no doubt that automated patch clamp (APC) technology has revolutionized research in biomedical science. High throughput ion channel screening is now an integral part of the development and safety profiling of the majority of new ch...