Comprehensive protocol for culturing and functionally characterizing primary mixed neural cells from the neonatal rat cortex [0.03%]
来自新生大鼠皮层的主要混合神经元的培养和功能表征的全面方案
Thaynan Silva Ramos,Matheus Heidemann Tempone,Hercules Rezende Freitas et al.
Thaynan Silva Ramos et al.
In vitro models using purified neurons or glial cells are crucial for studying neurological functions but often overlook intercellular interactions. Mixed neural cell cultures offer a more physiologically relevant system by preserving cell-...
Therapeutic drug monitoring of busulfan and cyclophosphamide in hematologic malignancies: An LC-MS/MS development, validation and application [0.03%]
用于血液系统恶性肿瘤中巴利昔尤单抗和环磷酰胺治疗药物监测的LC-MS/MS法的开发、验证及应用研究
Jinxingyi Wang,Ruyu Tao,Hanshuai Hu et al.
Jinxingyi Wang et al.
Background: Busulfan and cyclophosphamide (Bu-Cy) is a widely used conditioning regimen for hematopoietic stem cell transplantation (HSCT) in the treatment of hematologic malignancies. To explore the exposure-efficiency r...
Evaluation of the Effects of cypermethrin Toxicity on Liver Mitochondria of Male Albino Rats [0.03%]
评估氯氰聚酯毒性对雄性白鼠肝线粒体的影响
Venkataramanaiah Poli,Srinivasulu Reddy Motireddy
Venkataramanaiah Poli
The purpose of this study is to look into the toxic effects of giving male albino rats cypermethrin (CPM). In order to achieve this, three groups of rats were formed. Every group received the same amount of saline, including the control gro...
Legacy hERG study data in contemporary integrated risk assessments - mitigating the risk of protocol and positive control article differences [0.03%]
经典hERG研究数据在现代综合风险评估中的应用——减轻协议和阳性对照文章差异的风险
Richard W Daniels,James W Kramer,Matthew M Abernathy et al.
Richard W Daniels et al.
Testing new molecules against the hERG potassium channel has been a routine component of secondary pharmacology assessments for more than a quarter of a century. In 2005, the ICH S7B guidance required a GLP hERG study prior to first adminis...
Spatial regulation of CD14-like epitope in hematopoietic and ocular tissues of cultured North Atlantic lumpfish (Cyclopterus lumpus) and spotted wolffish (Anarhichas minor) during bacterial infection and temperature variations [0.03%]
北大西洋团突鱼(Cyclopterus lumpus)和点纹狼鱼(Anarhichas minor)在细菌感染及温度变化时造血组织和眼组织中CD14-like表位的空间调节
Rebecca R Kwabiah,Lauren E Murphy,Hélène Paradis et al.
Rebecca R Kwabiah et al.
The development of new tools for a better understanding of innate immune mediated responses to tissue damage in emerging model organisms could offer novel approaches to inform toxicology, disease mitigation and/or drug discovery. Innate imm...
A retrospective analysis of the concordance between cardiovascular effects in telemetry studies, toxicity studies, and early clinical trials [0.03%]
遥测研究、毒性研究及早期临床试验中心血管影响的一致性回顾分析
Andrea Greiter-Wilke,Jan Attig,Simon Bassett
Andrea Greiter-Wilke
This work aimed to compare the ability of several ECG technologies used in toxicity studies to identify cardiovascular effects. A retrospective analysis of 41 drug development projects across the Roche portfolio from 2005 to 2023 was conduc...
Improved translation of Nav1.5 channel inhibition to in vivo QRS interval prolongation via the hIPSC cardiomyocyte model [0.03%]
人诱导多能干细胞心肌细胞模型改善Nav1.5通道抑制的临床前评价效能
John P Imredy,Holly Clouse,Mei Zhang et al.
John P Imredy et al.
Introduction: Drug risk assessment to ventricular conduction typically involves measuring functional inhibition of the cardiac sodium channel (Nav1.5) followed by nonclinical in vivo assessment of prolongation of the elec...
Metabolomics using high-resolution magic angle spinning nuclear magnetic resonance: Neurotoxicological applications [0.03%]
基于高分辨率魔角旋转核磁共振的代谢组学在神经毒理学中的应用
Rachel Neita,Kenna Hynes,Grace V Mercer et al.
Rachel Neita et al.
High-resolution magic angle spinning nuclear magnetic resonance (HRMAS NMR) is a cutting-edge technology for metabolomics studies that has found application in pharmacology and toxicology. Metabolomics provides insight into how biological s...
Thermal shift assays in drug discovery - Troubleshooting from biochemical to cellular applications [0.03%]
药物发现中的热移位测定法-从生化应用到细胞应用的故障排除
Danielle Hanke,Jennifer I Brown,Brent D G Page
Danielle Hanke
Thermal shift assays (TSAs) have become valuable tools within drug discovery and development pipelines. These techniques detect direct physical interactions between small, drug-like molecules and their target proteins in biochemical and cel...
Anticoagulant activity of Eucalyptus essential oils: An in vitro approach and a bioinformatics-based pharmacokinetic-pharmacodynamic analysis [0.03%]
基于生物信息学的药代-药效分析及尤加利精油的体外抗凝血活性研究
Jhonatan Felipe Dos Santos,Maria Eduarda Dias Possate,Maysa Barbosa de Almeida et al.
Jhonatan Felipe Dos Santos et al.
Coagulation disorders pose significant health risks, requiring effective therapies. Natural products emerge as promising anticoagulant agents, standing out in the search for innovative and safe alternatives, integrating in vitro studies, ph...