Contractility assessment of human iPSC-derived cardiomyocytes by using a motion vector system and measuring cell impedance [0.03%]
基于运动向量系统和细胞阻抗测量的人诱导多能干细胞源性心肌细胞收缩力评价方法
Ayano Satsuka,Sayo Hayashi,Shota Yanagida et al.
Ayano Satsuka et al.
Predicting drug-induced cardiotoxicity during the non-clinical stage is important to avoid severe consequences in the clinical trials of new drugs. Human iPSC-derived cardiomyocytes (hiPSC-CMs) hold great promise for cardiac safety assessme...
Development of a medium throughput whole-cell microtiter plate Thr286 autophosphorylation assay for CaMKIIα using ELISA [0.03%]
一种基于ELISA的CaMKIIα Thr286自身磷酸化微孔板中通量检测方法的建立
Line B Palmelund,Geeske M van Woerden,Hans Bräuner-Osborne et al.
Line B Palmelund et al.
Ca2+/calmodulin-dependent protein kinase II alpha (CaMKIIα) is a multifunctional Ser/Thr kinase involved in several neuronal signaling pathways including synaptic plasticity. CaMKIIα autonomous activity is highly dependent on Thr286 autop...
A mouse model of allergic conjunctivitis permitting tear eosinophil quantification [0.03%]
一种允许量化泪液嗜酸性粒细胞的过敏性结膜炎小鼠模型
Atsushi Ogura,Yukio Sugimoto
Atsushi Ogura
Introduction: Allergic conjunctivitis is an immune-mediated inflammatory disease of the conjunctiva that is induced by antigens. Allergic conjunctivitis can cause various symptoms such as ocular itching, hyperemia and ede...
Less invasive, simultaneous, and continuous measurements of locomotor activity and body temperature using the nano tag® small accelerometer device in cynomolgus monkeys [0.03%]
用于食蟹猴同时连续测量运动和体温的nano tag小型加速度计设备的微创应用
Tomoyuki Kishida,Yoshiyuki Motokawa,Ryohei Yokoi et al.
Tomoyuki Kishida et al.
Locomotor activity and body temperature evaluations of cynomolgus monkeys are useful to understand the effects of drugs on the central nervous system. Here, we describe a simple, inexpensive, and less invasive evaluation method using the na...
Electrocardiography and heart rate variability in Göttingen Minipigs: Impact of diurnal variation, lead placement, repeatability and streptozotocin-induced diabetes [0.03%]
日间节律、电极放置和重复性对吉特灵迷你猪心电图及心率变异性的影响以及链球菌诱导的糖尿病对其影响
Mille Kronborg Lyhne,Karina Poulsdóttir Debes,Terese Helgogaard et al.
Mille Kronborg Lyhne et al.
Background: The Göttingen Minipig is widely used in preclinical research and safety pharmacology, but standardisation of porcine electrocardiography (ECG) is lacking. The aim of this study was to investigate diurnal effe...
Use of high throughput ion channel profiling and statistical modeling to predict off-target arrhythmia risk - One pharma's experience and perspective [0.03%]
高通量离子通道筛选结合统计学模型预测脱靶致心律失常风险的一家制药企业的经验与观点
Eric D Watt,Tiffany Lee,Shuyun Lily Feng et al.
Eric D Watt et al.
Introduction: The use of high throughput patch clamp profiling to determine mixed ion channel-mediated arrhythmia risk was assessed using profiling data generated using proprietary internal and clinical reference compound...
Amphetamine, methamphetamine, and MDMA in hair samples from a rehabilitation facility: Validation and applicability of HF-LPME-GC-MS [0.03%]
用于康复中心毛发样本中苯丙胺、甲基苯丙胺和MDMA检测的HF-LPME-GC-MS方法验证及应用研究
Mariana A O Madia,Luís O de Oliveira,Nicole S Baccule et al.
Mariana A O Madia et al.
Introduction: It is known that drug abuse jeopardizes economic and social development. Toxicological analyses can guide prevention and treatment strategies in rehabilitation facilities. The current greatest challenge is f...
Development of a bridging ELISA for detection of antibodies against ZV0203 in cynomolgus monkey serum [0.03%]
用于检测食蟹猴血清中针对ZV0203抗体的桥联酶联免疫吸附法的发展
Tingting Liu,Yongliang Tong,Jinli Gao et al.
Tingting Liu et al.
Immunogenicity has been a major concern in the safety evaluation of therapeutic proteins. The assessment of the unwanted immunogenicity of the therapeutic proteins performed in animals prior to clinical trials has been a regulatory requirem...
Standardization and validation of novel ex-vivo method for mitochondrial bioenergetics using mitochondrial modulators [0.03%]
基于线粒体调节剂的新型线粒体生物能学检测方法的标准化和验证
Prabha Rajput,Sairam Krishnamurthy
Prabha Rajput
Mitochondria is an essential organelle; it produces 95% of the adenine triphosphate (ATP) of cells, their dysfunction is related to the pathogenesis of multiple diseases, such as diabetes mellitus, cardiovascular and neurological disorders....
Nicholas D Ether,Derek J Leishman,Marc B Bailie et al.
Nicholas D Ether et al.
Preclinical risk assessment of drug-induced arrhythmias is critical for drug development and relies on heart rate corrected QT interval (QT) prolongation as a biomarker for arrhythmia risk. However, the methods used to correct QT vary in co...