Silicon biology in crops under abiotic stress: A paradigm shift and cross-talk between genomics and proteomics [0.03%]
非生物胁迫下作物的硅生物学：范式转换及基因组学与蛋白质组学的相互作用
Kuppan Lesharadevi,Theivasigamani Parthasarathi,Sowbiya Muneer
Kuppan Lesharadevi
Silicon is a beneficial element to improve the biological process, growth, development, and crop productivity. The review mainly focuses on the advantage of crops supplemented with silicon, how Si alleviate abiotic stress as well as regulat...
A simple and low-cost resazurin assay for vitality assessment across species [0.03%]
一种简单低成本的评估不同物种活力的试剂盒检测法
A Mehring,N Erdmann,J Walther et al.
A Mehring et al.
Working with biological organisms requires knowledge about the state of their viability and vitality to ascertain efficient processes. The phenoxazine dye resazurin is routinely used for viability assessment of many different species. Here,...
Assembly and characterization of the genome of chard (Beta vulgaris ssp. vulgaris var. cicla) [0.03%]
甜菜(vulgaris ssp. vulgaris var. cicla)基因组的组装和特征分析
Reinhard Lehner,Lisa Blazek,André E Minoche et al.
Reinhard Lehner et al.
Chard (Beta vulgaris ssp. vulgaris var. cicla) is a member of one of four different cultigroups of beets. While the genome of sugar beet, the most prominent beet crop, has been studied extensively, molecular data on other beet cultivars is ...
Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies [0.03%]
NS0和CHO细胞培养的策略性喂养以控制单克隆抗体的聚糖谱型和免疫原性抗原表位
Carina Villacrés,Venkata S Tayi,Michael Butler
Carina Villacrés
The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0. Of particular concern is the potential for genera...
Removal of bacterial dextran in sugarcane juice by Talaromyces minioluteus dextranase expressed constitutively in Pichia pastoris [0.03%]
一株分泌型表达细菌糊精酶的毕赤酵母用于甘蔗汁中降解多糖污染物
Duniesky Martínez,Carmen Menéndez,Osmani Chacón et al.
Duniesky Martínez et al.
A gene construct encoding the mature region of Talaromyces minioluteus dextranase (EC 3.2.1.11) fused to the Saccharomyces cerevisiae SUC2 signal sequence was expressed in Pichia pastoris under the constitutive glyceraldehyde 3-phosphate de...
Enhancement of catabolite regulatory genes in Saccharomyces cerevisiae to increase ethanol production using hydrolysate from red seaweed Gloiopeltis furcata [0.03%]
利用红藻Gloiopeltis furcata水解液提高Saccharomyces cerevisiae乙醇产量的底物阻遏基因改良方法
Yu Rim Park,Ji Won Yang,In Yung Sunwoo et al.
Yu Rim Park et al.
Glucose and galactose are monosaccharides obtained from Gloiopeltis furcata (Red algae). A total monosaccharide yield of 62.3 g/L was obtained from G. furcata using thermal acid hydrolysis and enzymatic saccharification. Activated carbon wa...
Immobilized lipase catalytic synthesis of phenolamides and their potential against α-glucosidase [0.03%]
固定化脂肪酶催化合成苯胺衍生物及其α-糖苷酶抑制活性潜力
Fei Zeng,Haokuan Zhang,Mingming Xu et al.
Fei Zeng et al.
Although coumaroyltyramine (CT) derivatives are one kind of phenolamides with remarkable biological activities, the low content in plants would inhibit their potential use in food and pharmaceutical industries. Therefore, it is necessary to...
Novel intein-based self-cleaving affinity tag for recombinant protein production in Escherichia coli [0.03%]
新型intein-Based自切割亲和标签：在大肠杆菌中用于重组蛋白质表达及纯化的新工具
Marilla Amaranto,Paula Vaccarello,Elisa M E Correa et al.
Marilla Amaranto et al.
We evaluated several intein-based self-cleaving affinity tags for expression and single-step affinity chromatography purification of recombinant proteins produced in Escherichia coli. We used human growth hormone (hGH) as target protein tha...
Overexpression of rice OsWNK9 promotes arsenite tolerance in transgenic Arabidopsis plants [0.03%]
水稻OsWNK9过表达转基因拟南芥植物可提高其砷酸盐耐性
Rakesh Manuka,Ankush Ashok Saddhe,Ashish Kumar Srivastava et al.
Rakesh Manuka et al.
Protein kinases are involved in the transfer of phosphate group to serine, threonine, and tyrosine residues of a target protein. With No Lysine (WNK) kinase is a member of the serine/threonine protein kinase family, which has conserved cata...
Automated multi-scale cascade of parallel stirred-tank bioreactors for fast protein expression studies [0.03%]
一种用于快速蛋白质表达研究的自动化多尺度并行搅拌罐生物反应器级联设备
Nikolas Von den Eichen,Lukas Bromig,Valeryia Sidarava et al.
Nikolas Von den Eichen et al.
Automation, parallelization and autonomous operation of standard lab equipment, usually applied for manual bioprocess development, is considered as the key for reduction of bioprocess development time and costs. An automated bioreactor syst...