Accurate definition of control strategies using cross validated stepwise regression and Monte Carlo simulation [0.03%]
基于交叉验证逐步回归和蒙特卡洛模拟的控制策略的精确定义
Patrick Y Yang,Cerintha J Hui,Daniel J Tien et al.
Patrick Y Yang et al.
Drug manufacturing processes must consistently deliver safe and effective product. A key part of achieving this is process validation utilizing Quality by Design (QbD) principles. To meet process validation requirements, process characteriz...
Ex-foliar application of glycine betaine and its impact on protein, carbohydrates and induction of ROS scavenging system during drought stress in flax (Linum usitatissimum) [0.03%]
甜菜碱叶面喷施对亚麻干旱胁迫下蛋白质、碳水化合物及抗氧化系统的影响研究
Payal Gupta,Rhitu Rai,Sujata Vasudev et al.
Payal Gupta et al.
Crop plants have an innate capacity to acclimatize and survive myriad stresses in field conditions. This acclimatization to stress enhances crop stand in field and productivity of plant. Inter alia field crops withstand drought stress (hydr...
Impact of nitrogen deficiency on succinic acid production by engineered strains of Yarrowia lipolytica [0.03%]
氮缺乏对改性酿酒酵母生产琥珀酸的影响
Guillaume Billerach,Laurence Preziosi-Belloy,Carol Sze Ki Lin et al.
Guillaume Billerach et al.
Yarrowia lipolytica strains PGC01003 and PGC202 engineered for succinic acid production were studied and compared to the wild type strain W29. For the first time, these two strains were characterized in a chemically defined medium. Strain g...
Microfluidic electrical cell lysis for high-throughput and continuous production of cell-free varicella-zoster virus [0.03%]
用于高通量和连续生产无细胞带状疱疹病毒的微流体电细胞裂解法
Eun-Jae Won,Duc Anh Thai,Duong Duy Duong et al.
Eun-Jae Won et al.
Varicella-zoster virus (VZV), the causative agent of varicella and herpes zoster, is highly cell-associated and spreads via cell-to-cell contact in tissue culture. The lack of cell-free VZV hampers studies on VZV biology as well as antivira...
Hydrogen gas as a central on-off functional switch of reversible metabolic arrest - New perspectives for biotechnological applications [0.03%]
氢气作为一种可逆代谢休眠的中心开关-生物技术应用的新视角
Sotirios Zerveas,Evaggelos Kydonakis,Melpomeni-Sofia Mente et al.
Sotirios Zerveas et al.
Metabolism is the sum of all chemical reactions that sustain life. There is an ongoing effort to control metabolic rate, which correlates with the maximum lifespan potential and constitutes one of the oldest scientific questions. Herein, we...
Martina Zimmermann,Harald Kolmar,Aline Zimmer
Martina Zimmermann
For the generation of therapeutic proteins in cell culture, high producing clones are used. These clones have a high demand in amino acids to support cell growth and productivity. l-cysteine (Cys) is critical in highly concentrated feeds du...
Removal of overpainting from an historical painting of the XVIII Century: A yeast enzymatic approach [0.03%]
十八世纪历史油画上去除过度绘制：一种酵母酶方法
Zuzana Kisová,Jelena Pavlović,Lucia Šefčiková et al.
Zuzana Kisová et al.
Biocleaning of cultural heritage items is mainly performed using living microorganisms. Approaches utilizing the enzymes of isolated microorganisms have not been frequently investigated. To find an enzymatic alternative for the removal of a...
Glycolate production by a Chlamydomonas reinhardtii mutant lacking carbon-concentrating mechanism [0.03%]
缺乏碳浓缩机制的裂殖壶藻突变体产生甘乳糖酸
Eun Ju Yun,Guo-Chang Zhang,Christine Atkinson et al.
Eun Ju Yun et al.
The green alga Chlamydomonas reinhardtii serves as a model organism for plant and photosynthesis research due to many commonalities in metabolism and to the fast growth rate of C. reinhardtii which accelerates experimental turnaround time. ...
Automation of high CHO cell density seed intensification via online control of the cell specific perfusion rate and its impact on the N-stage inoculum quality [0.03%]
采用在线控制细胞特异性灌流速率实现高CHO细胞密度种子扩增培养的自动化及其对N级种子罐质量的影响
Markus Schulze,Johannes Lemke,David Pollard et al.
Markus Schulze et al.
Current CHO cell production processes require an optimized space-time-yield. Process intensification can support achieving this by enhancing the productivity and improving facility utilization. The use of perfusion at the last stage of the ...
Optically pure lactic acid production from softwood-derived mannose by Pediococcus acidilactici [0.03%]
嗜酸乳杆菌利用木糖生产光学纯乳酸
Joana Campos,Jie Bao,Gunnar Lidén
Joana Campos
Softwood is of interest as a renewable carbon source for production of lactic acid. Softwood hydrolysate contains a high content of mannose. Lactic acid production from mannose by two modified strains, L-lactic acid producing Pediococcus ac...