Development of a rapid method for site-directed mutagenesis in Streptococcus zooepidemicus [0.03%]
development of a rapid method for site-directed mutagenesis in streptococcus zooepidemicus（里氏木霉tranc端粒重复序列的克隆及功能分析）
Zbyněk Černý,Jana Jílková,Sofia Chatzigeorgiou et al.
Zbyněk Černý et al.
This paper describes the development of a straightforward method for site-directed gene mutagenesis in Streptococcus zooepidemicus, inspired by the mechanism of natural competence regulated by ComX in other streptococci. An alternative sigm...
Efficient laccase/TEMPO oxidation of alkyl glycosides: Effects of carbohydrate group and alkyl chain length [0.03%]
高效的漆酶/TEMPO氧化烷基糖苷的作用：碳水化合物基团和烷基链长度的影响
Ngoc T N Ngo,Carl Grey,Patrick Adlercreutz
Ngoc T N Ngo
Alkyl glycosides with long hydrophobic chains have attractive surfactant properties, but their wider application is hampered by their low solubility in water. Here, a route to increased solubility by introduction of carboxyl groups via lacc...
José M Escribano,Miguel Cid,Edel Reytor et al.
José M Escribano et al.
The baculovirus vector expression system (BEVS) combines cultured insect cells and genetically modified Autographa californica nuclear polyhedrosis virus (AcMNPV)-derived baculovirus vectors. This expression system has been widely used for ...
WITHDRAWN: Efficient enzyme formulation promotes Leloir glycosyltransferases for glycoside synthesis [0.03%]
撤稿：高效的酶制剂促进Leloir糖基转移酶用于糖苷合成
Markus Mikl,Alexander Dennig,Bernd Nidetzky
Markus Mikl
The Publisher regrets that this article is an accidental duplication of an article that has already been published in BIOTEC, 322C (2020) 74-78, https://doi.org/10.1016/j.jbiotec.2020.06.023. The duplicate article has therefore been withdra...
Towards industrial production of microalgae without temperature control: The effect of diel temperature fluctuations on microalgal physiology [0.03%]
微藻工业生产中无需温度控制的情况研究——昼夜温度波动对微藻生理的影响
Robin Barten,Yovita Djohan,Wendy Evers et al.
Robin Barten et al.
Regions that offer high levels of sunlight are ideal to produce microalgae. However, as a result of high light intensities, the temperature in photobioreactors can reach temperatures up to 50 °C. Control of temperature is essential to avoi...
Design of bioreactor based on immobilized laccase on silica-chitosan support for phenol removal in continuous mode [0.03%]
一种基于壳聚糖-硅胶固定漆酶的反应器连续去除苯酚的工艺设计
A M Girelli,L Quattrocchi,F R Scuto
A M Girelli
A silica-chitosan support was employed for laccase immobilization. The hybrid support was obtained using calcium ion as linking agent that coordinates silanol and hydroxyl groups of chitosan. The insoluble biocatalyst was then packed in a c...
In vivo immobilized carbonic anhydrase and its effect on the enhancement of CO2 absorption rate [0.03%]
固态 immobilized Carbonic Anhydrase 及其对CO2吸收速率的影响研究
S Fabbricino,S Del Prete,M E Russo et al.
S Fabbricino et al.
Reactive absorption into aqueous solutions promoted by carbonic anhydrase (CA, E.C. 4.2.1.1.) has been often proposed as a post-combustion CO2 capture process. The state of the art reveals the need for efficient biocatalyst based on carboni...
Improving mAb capture productivity on batch and continuous downstream processing using nanofiber PrismA adsorbents [0.03%]
利用纳米纤维PrismA吸附剂提高批处理和连续下游工艺中单克隆抗体的捕获效率
Ryan R Davis,Fletcher Suber,Ian Heller et al.
Ryan R Davis et al.
Improving productivity and decreasing costs for biotherapeutic agents has been a focal driving force in the manufacturing of biologics. Advances in upstream processes have been continuously outpacing the ability for downstream operations to...
Conjugation of haloalkane dehalogenase DhaA with arabinogalactan to increase its stability [0.03%]
脯聚糖结合卤代烷烃脱卤酶DhaA以提高其稳定性
Meiqi Wang,Weili Yu,Lijuan Shen et al.
Meiqi Wang et al.
Haloalkane dehalogenase DhaA can catalyze the hydrolytic cleavage of carbonhalogen bonds, along with production of the corresponding alcohol, a proton and a halide. However, DhaA suffers from poor environmental tolerance, such as sensitivit...
Host nutrition-based approach for biotechnological production of the antifungal cyclic lipopeptide jagaricin [0.03%]
基于宿主营养的抗真菌环状脂肽jagaricin的生物技术生产方法
Nicolas Schlosser,Jordi Espino-Martínez,Florian Kloss et al.
Nicolas Schlosser et al.
In today's, society multi-resistant pathogens have become an emerging threat, which makes the search for novel anti-infectives more urgent than ever. A promising class of substances are cyclic lipopeptides like the antifungal jagaricin. Jag...