Selection of CHO host and recombinant cell pools by inhibition of the proteasome results in enhanced product yields and cell specific productivity [0.03%]
通过抑制蛋白酶体选择CHO宿主和重组细胞群可提高产物滴度和细胞生产力
Tanya J Knight,Sarah Turner,Colin M Jaques et al.
Tanya J Knight et al.
Chinese hamster ovary (CHO) cells are the leading mammalian cell expression platform for biotherapeutic recombinant molecules yet some proteins remain difficult to express (DTE) in this, and other, systems. In recombinant cell lines express...
Targeted integration into pseudo attP sites of CHO cells using CRISPR/Cas9 [0.03%]
用于CHO细胞假attP位点定点整合的CRISPR/Cas9系统
Sana Pourtabatabaei,Samaneh Ghanbari,Narges Damavandi et al.
Sana Pourtabatabaei et al.
Chinese hamster ovary (CHO) cells are regarded as a prominent host for manufacturing therapeutic proteins. Although conventional strategies for generating recombinant proteins in CHO cells depend on the random integration of a gene of inter...
Hydrophobic interaction chromatography as polishing step enables obtaining ultra-pure recombinant antibodies [0.03%]
亲水作用色谱 polish 步骤可获得超高纯度的重组抗体
Iara Rocha Antunes Pereira Bresolin,Nico Lingg,Igor Tadeu Lazzarotto Bresolin et al.
Iara Rocha Antunes Pereira Bresolin et al.
Hydrophobic interaction chromatography is a versatile method to polish antibodies. Here, we present a polishing procedure in order to obtain an ultra-pure preparation of antitumor necrosis factor (TNF) alpha IgG1. Hydrophobic interaction ch...
Comparison of two glycoengineering strategies to control the fucosylation of a monoclonal antibody [0.03%]
两种糖基工程策略控制单克隆抗体岩藻糖基化的比较研究
Neha Mishra,Maureen Spearman,Lynda Donald et al.
Neha Mishra et al.
Core fucosylation of an Fc N-linked glycan affects antibody effector functions, as the absence of fucose increases the antibody dependent cell cytotoxicity (ADCC) response with increased binding to the Fcγ receptors. The work presented her...
Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds [0.03%]
基于结合的聚-3-羟基丁酸酯/脂质体的天然酚类化合物有机紫外线滤剂的制备及特征分析
Renata Pavelkova,Petra Matouskova,Julie Hoova et al.
Renata Pavelkova et al.
In this study preparation and characterization of new UV-protecting systems based on liposomes/polyhydroxybutyrate (PHB) with encapsulated coffee extracts are presented. Green and roasted coffee extracts with high phenolics content, high an...
Culture medium density as a simple monitoring tool for cell integrity of Escherichia coli [0.03%]
浊度作为监测大肠杆菌细胞完整性的简便工具
Jens Kastenhofer,Oliver Spadiut
Jens Kastenhofer
During the expression of recombinant proteins in the periplasm of Escherichia coli, the integrity of the outer membrane can change, so that product leaks to the medium. Additional stress can induce lysis, the complete disintegration of both...
Simultaneous growth and poly(3-hydroxybutyrate) (PHB) accumulation in a Plasticicumulans acidivorans dominated enrichment culture [0.03%]
聚羟基丁酸(PHB)积累与Plasticicumulans acidivorans富集培养的同步增长关系研究
Michel Mulders,Jelmer Tamis,Gerben Roelandt Stouten et al.
Michel Mulders et al.
The wide variety of organic carbon to nitrogen and phosphorous ratios that are encountered in different wastewaters has a major impact on the poly(3-hydroxybutyrate) (PHB) accumulation potential of microbial communities. In this study we in...
High pressure homogenization is a key unit operation in inclusion body processing [0.03%]
高压均质化是包涵体处理的关键单元操作
Britta Eggenreich,David Johannes Wurm,Vignesh Rajamanickam et al.
Britta Eggenreich et al.
Recombinant protein production in E. coli often leads to the formation of inclusion bodies (IBs). Although downstream processing of IBs has the reputation of being a great hurdle, advantages of IBs can be substantial. Highly pure recombinan...
Fluorescence responsiveness of unicellular marine algae Dunaliella to stressors under laboratory conditions [0.03%]
实验室条件下微藻对胁迫因子的荧光响应研究
Zuzana Pavlinska,Dusan Chorvat Jr,Anton Mateasik et al.
Zuzana Pavlinska et al.
We examined the responsiveness of unicellular green alga Dunalliela tertiolecta to selected stressors employing confocal- and time-resolved imaging of endogenous fluorescence. Our aim was to monitor cell endogenous fluorescence changes unde...
A bead-based method for the removal of the amino acid lysine from cell-free transcription-translation systems [0.03%]
一种从无细胞转录/翻译系统中去除赖氨酸的方法
Marc Finkler,Ömer Kurt,Florent Grimm et al.
Marc Finkler et al.
Cell-free transcription-translation systems are a versatile tool to study gene expression, enzymatic reactions and biochemical regulation mechanisms. Because cell-free transcription-translation systems are often derived from cell lysates, m...