A SacB-based system for diverse and multiple genome editing in Gluconobacter oxydans [0.03%]
基于SacB的葡萄球菌氧化亚族全基因组多样性编辑系统
Zhijie Qin,Shiqin Yu,Li Liu et al.
Zhijie Qin et al.
Gluconobacter oxydans is an important industrial bacterial strain widely used to produce a lot of useful products. However, very few gene editing tools are available for G. oxydans. This study aimed to develop an efficient genome editing me...
Microbial fuel cells for remediation of environmental pollutants and value addition: Special focus on coupling diatom microbial fuel cells with photocatalytic and photoelectric fuel cells [0.03%]
用于环境污染修复和增值的微生物燃料电池：以硅藻微生物燃料电池与光电催化燃料电池耦合为研究重点
Vandana Vinayak,Mohd Jahir Khan,Sunita Varjani et al.
Vandana Vinayak et al.
With the advent of global industrialisation and adaptation of smart life there is rise in anthropogenic pollution especially in water. Remediation of the pollutants (such as metals, and dyes) present in industrial effluents is possible via ...
Production of C20, C30 and C40 terpenes in the engineered phototrophic bacterium Rhodobacter capsulatus [0.03%]
产红细菌工程菌的C20、C30和C40倍半萜的合成
Jennifer Hage-Hülsmann,Oliver Klaus,Karl Linke et al.
Jennifer Hage-Hülsmann et al.
Terpenes constitute one of the largest groups of secondary metabolites that are used, for example, as food-additives, fragrances or pharmaceuticals. Due to the formation of an intracytoplasmic membrane system and an efficient intrinsic tetr...
Genetic incorporation of oil-utilizing ability in Cupriavidus malaysiensis USMAA2-4 for sustainable polyhydroxyalkanoates production from palm olein and 1-pentanol [0.03%]
遗传利用油类特性在Cupriavidus malaysiensis USMAA2-4中可持续地利用棕榈油脚和1-戊醇生产聚羟基脂肪酸酯
Hau Seung Jeremy Wong,Kai Hee Huong,Nur Asilla Hani Shafie et al.
Hau Seung Jeremy Wong et al.
The sustainability in polyhydroxyalkanoates (PHA) production is drawing increasing attention as the effort to increase the economic feasibility for commercialization pursues. Oleic acid is widely preferred by bacteria but its employment for...
Expression and characterization of a novel single-chain anti-vascular endothelial growth factor antibody in the goat milk [0.03%]
山羊奶中新型单链抗血管内皮生长因子抗体的表达及特性分析
María J Leiva,Natalie C Parra,María A Contreras et al.
María J Leiva et al.
Vascular endothelial growth factor (VEGF) has essential functions in angiogenesis, endothelial cell proliferation, migration, and tumor invasion. Different approaches have been developed to suppress tumor angiogenesis, which is considered a...
Highly efficient whole-cell biosynthesis and cytotoxicity of esculin esters [0.03%]
高效 esculin 酯的全细胞生物合成及其细胞毒性研究
Lisha Hao,Mengmeng Zhang,Xiaofeng Li et al.
Lisha Hao et al.
Esculin is a polyphenol with multiple bioactivities and poor lipophilicity. Therefore, a whole-cell catalytic strategy for esculin acylation was developed to improve its lipophilicity. A total of 12 strains were tested, among which Pseudomo...
Affinity precipitation of monoclonal antibodies using ELP-Z in the elution without resolubilization mode [0.03%]
使用ELP-Z在无需复溶模式下进行单克隆抗体的亲和沉淀纯化
Manish Bhat,Akshat Mullerpatan,Jie Chen et al.
Manish Bhat et al.
This paper describes a simplified affinity precipitation process for the purification of mAbs from complex mixtures using elastin-like polypeptide fused to a single Z domain of protein A (ELP-Z). This approach eliminates several steps in th...
A mutant T1 lipase homology modeling, and its molecular docking and molecular dynamics simulation with fatty acids [0.03%]
T1型脂肪酶突变体同源模建及其与脂肪酸的分子对接和分子动力学模拟研究
Xiaoli Qin,Jinfeng Zhong,Yonghua Wang
Xiaoli Qin
A thermostable T1 lipase from Geobacillus zalihae exhibits broad substrate specificity and good potential application in fats and oils. However, structural insight into the enzyme against substrates is poorly understood at the molecular lev...
Role of nanoparticles in crop improvement and abiotic stress management [0.03%]
纳米颗粒在农作物改良和非生物胁迫管理中的作用
Archana Singh,Shalini Tiwari,Jyotsna Pandey et al.
Archana Singh et al.
Nanoparticles (NPs) possess specific physical and chemical features and they are capable enough to cross cellular barriers and show their effect on living organisms. Their capability to cross cellular barriers have been noticed for their ap...
Immobilization of Pseudomonas stutzeri lipase through Cross-linking Aggregates (CLEA) for reactions in Deep Eutectic Solvents [0.03%]
通过交联聚集体(CLEA)固定Pseudomonas stutzeri脂肪酶用于深配位溶剂中的反应
Nadia Guajardo,Katherine Ahumada,Pablo Domínguez de María
Nadia Guajardo
The use of deep eutectic solvents (DES) with buffer as cosolvent (up to 10 % v/v) leads to low-viscous media in which lipases can perform synthetic reactions, instead of hydrolysis. This paper explores the immobilization of Pseudomonas stut...