Improved production of D-pantothenic acid in Escherichia coli by integrated strain engineering and fermentation strategies [0.03%]
整合菌株工程和发酵策略提高大肠杆菌D泛酸产量
Shuping Zou,Kuo Zhao,Heng Tang et al.
Shuping Zou et al.
D-pantothenic acid (D-PA) is an essential vitamin that has been widely used in medicine, food, and animal feed. Microbial production of D-PA from natural renewable resources is attractive and challenging. In this study, both strain improvem...
A brief review on recent development of multidisciplinary engineering in fermentation of Saccharomyces cerevisiae [0.03%]
关于酿酒酵母发酵多学科工程进展的简述综述
Shiwen Zhuang,Neil Renault,Ian Archer
Shiwen Zhuang
Fermentation technology has unprecedented potential to upgrade state-of-art biotechnology and refine the processes used in existing ones, taking into account of complex technical, economic and environmental factors. Given the economic impor...
Recombinant Yarrowia lipolytica strains for the heterologous expression of multi-component enzyme systems: Expression of mammalian steroidogenic proteins [0.03%]
用于异源表达多组分酶系统的 recombinant Yarrowia lipolytica 菌株：哺乳动物类固醇生成蛋白的表达
Ludmila A Novikova,Venelina Yovkova,Valentin N Luzikov et al.
Ludmila A Novikova et al.
New Yarrowia lipolytica strains for the co-expression of steroidogenic mammalian proteins were obtained in this study. For this purpose, a two-step approach for constructing recombinant strains that permits the simple introduction of severa...
Enhancement of heterologous protein production in Corynebacterium glutamicum via atmospheric and room temperature plasma mutagenesis and high-throughput screening [0.03%]
大气及室温等离子促变及高通量筛选提升棒状杆菌异源蛋白表达能力
Lihong Meng,Xiong Gao,Xiuxia Liu et al.
Lihong Meng et al.
Atmospheric and room temperature plasma (ARTP) is a new and efficient mutation breeding technique. In this study, we discuss a strategy combining ARTP mutagenesis and high-throughput screening to engineer Corynebacterium glutamicum towards ...
A plant CitPITP1 protein-coding exon sequence serves as a promoter in bacteria [0.03%]
植物CitPITP1蛋白编码外显子序列在细菌中用作启动子
Qingjiang Wu,Jialing Fu,Juan Sun et al.
Qingjiang Wu et al.
Genetic manipulation of plant genes in prokaryotes has been widely used in molecular biology, but the function of a DNA sequence is far from being fully known. Here, we discovered that a plant protein-coding gene containing the CRAL_TRIO do...
Oxidative torrefaction performance of microalga Nannochloropsis Oceanica towards an upgraded microalgal solid biofuel [0.03%]
海洋微藻Nannochloropsis oceanica氧化热解特性及其固态生物燃料的升级研究
Congyu Zhang,Shih-Hsin Ho,Wei-Hsin Chen et al.
Congyu Zhang et al.
Microalgae are a promising feedstock for carbon-neutral biofuel production due to their superior cellular composition. Alternatively, oxidative torrefaction has been recognized as a potential thermochemical technique for microalgal solid bi...
Syntrophy of Crypthecodinium cohnii and immobilized Zymomonas mobilis for docosahexaenoic acid production from sucrose-containing substrates [0.03%]
产皮胶红酵母与固定化希瓦氏 Mobilis 酵母共培养利用含蔗糖基质生产二十二碳六烯酸
Inese Strazdina,Linards Klavins,Nina Galinina et al.
Inese Strazdina et al.
Marine heterotrophic dinoflagellate Crypthecodinium cohnii is an aerobic oleaginous microorganism that accumulates intracellular lipid with high content of 4,7,10,13,16,19-docosahexaenoic acid (DHA), a polyunsaturated ω-3 (22:6) fatty acid...
Simple, fast and efficient iTOP-mediated delivery of CRISPR/Cas9 RNP in difficult-to-transduce human cells including primary T cells [0.03%]
一种简单、快速和有效的人类难转染细胞（包括原代T细胞）的CRISPR / Cas9 RNP传递方法iTOP
Waleed M Kholosy,Marieke Visscher,Kim Ogink et al.
Waleed M Kholosy et al.
The advent of the CRISPR/Cas9 system has transformed the field of human genome engineering and has created new perspectives in the development of innovative cell therapies. However, the absence of a simple, fast and efficient delivery metho...
Generation of phytase chimeras with low sequence identities and improved thermal stability [0.03%]
低序列相似性且热稳定性提高的植酸酶嵌合体的生成
Kevin R Herrmann,Isabell Hofmann,Dennis Jungherz et al.
Kevin R Herrmann et al.
Being able to recombine more than two genes with four or more crossover points in a sequence independent manner is still a challenge in protein engineering and limits our capabilities in tailoring enzymes for industrial applications. By com...
Strategies to meet the global demand for natural food colorant bixin: A multidisciplinary approach [0.03%]
满足全球天然食品色素宾辛需求的战略：一种多学科方法
Leepica Kapoor,Siva Ramamoorthy
Leepica Kapoor
Bixin is an apocarotenoid derived from Bixa orellana L. well known as a food colorant along with its numerous industrial and therapeutic applications. With the current surge in usage of natural products, bixin has contributed immensely to t...