Autotrophic isopropanol production by Cupriavidus necator using industrial biogas and incinerator flue gases as carbon sources [0.03%]
以工业生物气和焚烧炉烟道气为碳源的自养异丙醇生产菌株杯隆微生物定殖者的自动合成异丙醇生产
I Weickardt,A Zhang,E Lombard et al.
I Weickardt et al.
Gas fermentation is a promising approach to reduce carbon emissions of production processes by using microorganisms to convert industrial gas streams into valuable carbon-based products. Here, isopropanol production by engineered Cupriavidu...
Identification of chalcopyrite-binding peptides for flotation applications using phage display and deep sequencing [0.03%]
基于噬菌体展示和深度测序技术识别黄铜矿结合多肽
Barbara Mejia Bohorquez,Thierry Vincent,Denise Tremblay et al.
Barbara Mejia Bohorquez et al.
Phage display is a powerful technology for identifying new ligands, including mineral-selective peptides. However, it is prone to several biases that may lead to false positives or false negatives, particularly due to non-specific binding, ...
Renato Rebimbas,Inês Glória,Júlia Chegão et al.
Renato Rebimbas et al.
The rapid expansion of global digital data generation has exposed critical limitations of conventional electronic storage technologies, including scalability, longevity, and sustainability constraints. Deoxyribonucleic acid (DNA) has emerge...
SynMicrobe-driven lignocellulose humification: Key enzymes, metabolic pathways, and two-stage degradation-reconstruction mechanisms revealed via metaproteomics [0.03%]
基于宏蛋白质组学的纤维素腐殖化驱动微生物及机制研究
Xiaoya Qin,Rixin Bao,Wenyu Huang et al.
Xiaoya Qin et al.
Although numerous studies have explored the role of microbial inoculants in organic matter decomposition, the detailed proteomic mechanisms and metabolic regulation networks underlying the transformation of lignocellulose into humus remain ...
Biotechnological parameters of cultivation of mycorrhizal fungi Cortinarius caperatus and Laccaria bicolor [0.03%]
肉桂红锈伞和松塔鞍菇菌根真菌的培养条件及参数研究
Dmitry Belousov,Nadezhda Filinova,Ludmila Belovezhets et al.
Dmitry Belousov et al.
The trend towards active reforestation is evident worldwide. A promising way to improve the quality of planting material is the forced mycorrhization of seedlings with mycorrhizal fungi. Cultures of Cortinarius caperatus and Laccaria bicolo...
Efficient expression and functional verification of recombinant human type Ⅲ collagen in CHO cells [0.03%]
CHO细胞中重组人III型胶原的高效表达及功能验证
Cong-Hui Luo,Qiu-Li Sun,Jun-Hao Liang et al.
Cong-Hui Luo et al.
Collagen is a structural protein and the main component of extracellular matrix. Collagen Ⅲ is twisted from three peptide chains to three spirals, which provides tensile strength and structural integrity for many organs, and participates i...
A stress-tolerant, chaperone-like peptidylprolyl isomerase (PPIase) from Chromohalobacter salexigens as a fusion partner for soluble, correctly folded expression in Escherichia coli [0.03%]
来源于 Chromohalobacter salexigens 的一种耐压热的类似分子伴侣型肽基-脯氨酰异构酶(PPIase)可作为融合蛋白改善 E. coli 中目的蛋白的溶解度及正确折叠
Azumi Nagaoka,Fumihiro Yamagami,Yuto Otsuka et al.
Azumi Nagaoka et al.
Halophilic bacteria have evolved exceptionally high protein solubility to withstand aggregation in high-salt environments. Leveraging this trait, we isolated a chaperone-like peptidylprolyl isomerase (PPIase) from the moderately halophilic ...
Engineering an aldoxime dehydratase with high activity and isomer tolerance for biosynthesis of an O-protected primary cyanohydrin [0.03%]
工程化构建高活性及异构体耐受性的亚胺类脱水酶用于O-保护的一级氢氰酸加成物生物合成
Marcus V Moreno,Daniel J Wackelin,Erick G Ortiz et al.
Marcus V Moreno et al.
O-protected primary cyanohydrins (glycolonitriles) are important building blocks for many difunctionalized compounds and precursors to known bioactive molecules. Their synthesis, however, utilizes toxic cyanide, which raises significant saf...
Development of Efficient Resin-Based Method for Purifying 3-Hydroxyphenazine-1-Carboxylic Acid from Aspergillus sclerotiorum ASJ82 Crude Extract [0.03%]
一种高效树脂法从Aspergillus sclerotiorum ASJ82粗提取物中分离纯化3-羟基菲嗪-1-甲酸的方法学研究
Malik Jan,Kashif Hayat,Israr Khan et al.
Malik Jan et al.
The new metabolite 3-hydroxyphenazine 1-carboxylic acid (3-OH-PCA) was obtained by Aspergillus sclerotiorum ASJ82 whole-cell biocatalysis of phenazine 1-carboxylic acid. Despite the biotechnological perspective, the lack of favorable method...
Persistent Low PHB Accumulation During Pilot-Scale Gas Fermentation in Cupriavidus necator [0.03%]
在恶臭假单胞菌中进行气体发酵中试过程中聚-3-羟基丁酸酯的持续高效积累
Daniel Schwendenwein,Kay Domenico Novak,Christoph Reisinger et al.
Daniel Schwendenwein et al.
Climate change and food security are tightly linked global challenges. Gas fermentation offers a sustainable route to convert CO₂ into microbial single-cell protein (SCP), with hydrogen-oxidizing bacteria (HOB) emerging as efficient biomas...