MS-based workflow for PTM profiling of rAAV capsid proteins to support bioprocess monitoring and development [0.03%]
基于修饰谱型的rAAV衣壳蛋白翻译后修饰分析工作流程以支持工艺表征及开发研究
Tomás M Mateus,Sofia B Carvalho,Bruno M Alexandre et al.
Tomás M Mateus et al.
Post-translational modifications (PTMs) of recombinant adeno-associated virus (rAAV) capsids have become a focus of interest due to their potential role in vector structure, stability, and function. However, consistent and comprehensive PTM...
Computational design of a single-chain galectin-1 yields a stable variant with retained glycan binding activity [0.03%]
计算设计单链半乳糖凝集素-1得到稳定性增强但结合活性不变的变异体
Dajana Kolanovic,Markus Braun,Rajeev Pasupuleti et al.
Dajana Kolanovic et al.
Developing galectin-1-based therapeutics faces challenges that are specific for this lectin, such as its differing redox states and reversible monomer-dimer equilibrium. The generation of a cysteine-less mutant of galectin-1 (CSGal-1) stabi...
Immortalization of mesenchymal stromal cells by hTERT does not affect the functional properties of secreted extracellular vesicles [0.03%]
端粒酶逆转录酶使间充质基质细胞获得永生化但不影响分泌的小胞外囊泡的功能性质
Alessia Brancolini,Madhusudhan Reddy Bobbili,Marianne Pultar et al.
Alessia Brancolini et al.
Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) have emerged as promising and safe therapeutic agents, however, donor heterogeneities, limited replicative life span and changes in the cellular phenotype throughout in vitro...
Peptonics: Shear protection and foam prevention in mammalian cell cultures producing monoclonal antibodies and viral vectors for gene therapy [0.03%]
肽类保护剂及其在基因治疗病毒载体和单克隆抗体哺乳动物细胞培养中应用申请答复通知书
Ka Zhang,Eduardo Barbieri,Arianna Minzoni et al.
Ka Zhang et al.
Biologics produced by cell culture are central to modern pharmaceutical production. In this context, the surfactants added to culture media for protecting cells from shear are essential for ensuring product yield and quality. Commercial sur...
Whole-cell biocatalysis of HMF into BHMF by Aspergillus niger: Process development and identification of key genes [0.03%]
Aspergillus niger全细胞生物催化HMF合成BHMF：工艺开发及关键基因识别
Hongli Zhang,Jinbao Yin,Yanqing Liu et al.
Hongli Zhang et al.
The conversion of 5-hydroxymethylfurfural (HMF), a key platform chemical derived from lignocellulosic biomass, into value-added products such as 2,5-bis(hydroxymethyl)furan (BHMF) is of great interest for sustainable biorefineries. In this ...
Luwen Zhang,Jiawei Tang,Songbai Yang et al.
Luwen Zhang et al.
Ergothioneine (ERG) is a natural sulfur-containing compound derived from histidine that is known for its significant antioxidant properties. Microbial biosynthesis of ERG has attracted considerable interest due to its sustainability and sca...
Ethanol alleviates glucose stress from Euglena gracilis [0.03%]
乙醇通过改善 Euglena gracilis 的葡萄糖应激来促进其生长发育
Minami Makimoto,Takashi Osanai
Minami Makimoto
Euglena gracilis, a microalga, can be cultured under photoautotrophic, heterotrophic, or mixotrophic conditions. This species can produce proteins, vitamins, and lipids. Based on previous reports, paramylon, a storage polysaccharide of E. g...
A Biocompatible and Flexible medium chain length PHA, P(3HB-co-20mol%3HHx) for Neural Tissue Engineering [0.03%]
一种用于神经组织工程的生物相容性和柔韧性中链长度PHA(P(3HB-co-20mol%3HHx))
Lara Santolin,Caroline S Taylor,Björn Weiske et al.
Lara Santolin et al.
Polyhydroxyalkanoates (PHAs) have shown much promise as materials in nerve tissue engineering. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] has excellent biocompatibility, biodegradability, and tuneable mechanical properti...
Co-culturing Bacteria with Monospecific Microalgal Biofilms for Improved Biomass and Lipid production [0.03%]
混合培养细菌与单种微藻菌胶团以提高生物量和脂质产量
Nadeeshani Dehel Gamage,Aurélie Mossion,Murtaza Khan et al.
Nadeeshani Dehel Gamage et al.
Integrating microbiome engineering into microalgal biofilms could offer an environmentally sustainable and economically viable strategy to enhance biomass and lipid yields while leveraging the inherent advantages of biofilm-based systems. B...
Enhanced anthraquinone biosynthesis in Damnacanthus major cells via medium optimization and fed-batch bioreactor culture [0.03%]
通过优化培养基和分批补料生物反应器培养增强 Damnacanthus major 细胞中蒽醌的生物合成
Chun-Yu Jiang,Xin-Yuan Wang,Yu-Qing Guo et al.
Chun-Yu Jiang et al.
Damnacanthus major is biotechnologically valued for its rich anthraquinone metabolites. However, limited wild resources necessitate the development of efficient cell culture production. This study first used single-factor experiments to det...