Evaluation of nanobodies engineered with extra-cysteines for site-specific functionalization [0.03%]
具有额外半胱氨酸的纳米抗体的工程改造及其定点修饰性能评估
Huyen Thuc Tran Luong,Urša Štrancar,Xinyu Hu et al.
Huyen Thuc Tran Luong et al.
Antibody labeling represents a critical step for the preparation of application-specific immunoreagents because the process can affect the binder functionality and result in a highly heterogeneous sample. Therefore, site-specific derivatiza...
Kai Wang,Zi-Wen Xie,Zheng-Heng Qian et al.
Kai Wang et al.
The trans-4-hydroxy-L-proline (i.e., T-4-Hyp), a key component of collagen, possesses broad application value in pharmaceuticals, food, cosmetics, and chemical industries. Traditional methods such as animal tissue extraction and chemical sy...
AbrB knockout not positively affecting bacillomycin D production in Bacillus amyloliquefaciens fmbJ analyzed by metabolomics [0.03%]
代谢组学分析表明在枯草芽孢杆菌fmbJ中敲除AbrB不会正向影响bacillomycin D的产生
Jing Sun,Shanshan Jin,Yujuan Liu et al.
Jing Sun et al.
Bacillomycin D (BD) is a potent lipopeptide with broad application potential encoded by bmyDABC with amino acids as substrates. To clarify the influence of transcriptional repressor AbrB regulating the synthesis of BD, abrB gene in Bacillus...
Cellulose-based biosensors exploiting diamine-oxidase and soybean peroxidase for biogenic amine determination in solution [0.03%]
基于丝氨酸氧化酶和大豆过氧化物酶的纤维素基生物传感器在溶液中用于生物胺测定
Maria Laura Tummino,Vincenzo Antonio Daniele,Enzo Laurenti et al.
Maria Laura Tummino et al.
Two biosensors with enzymes immobilized on fibrous cellulose powder and paper have been developed for the colorimetric detection of biogenic amines, which are of great interest for food and health security. These systems exploited diamine o...
Metabolic engineering of Pseudomonas putida KT2440 for upcycling of terephthalic acid into levulinic acid [0.03%]
以对苯二甲酸为原料生产富马酸的假单胞菌代谢工程菌株构建
Gaeun Lim,Hyunjin Kim,Yunhee Jeong et al.
Gaeun Lim et al.
Terephthalic acid (TPA) is an aromatic dicarboxylic acid that is widely used as a monomer in the production of polyesters such as polyethylene terephthalate (PET) and poly (butylene adipate-co-terephthalate) (PBAT). However, its chemical st...
Production of the anticancer drug intermediate strictosidinic acid in engineered yeast [0.03%]
在工程酵母中生产抗癌药物中间体番木鳖碱氮酸
Benedikt Seligmann,Shenyu Liu,Mai Huynh et al.
Benedikt Seligmann et al.
Strictosidinic acid is a key intermediate in the biosynthetic pathway of camptothecin, a plant alkaloid that serves as a precursor for semisynthetic anticancer drugs. At the moment, camptothecin is mainly sourced from trees, causing limited...
Discovery and characterization of a thermophilic endo-inulinase with exceptional thermostability for efficient inulooligosaccharide production [0.03%]
高效生产低聚果糖的嗜热内切果聚糖酶的挖掘与鉴定
Ziqing Gao,Wenjing Hu,Hao Zhu et al.
Ziqing Gao et al.
Endo-inulinases are key biocatalysts for converting inulin into prebiotic inulooligosaccharides (IOS). However, industrial implementation is hindered by a mismatch between inulin solubility and enzyme operating temperatures. Natural inulin ...
Enhancing the utility of Sendai virus-based vectors through antiviral compound-mediated removal [0.03%]
通过抗病毒化合物介导清除提高Sendai病毒载体的实用性
Emmanuel Osei Mensah,Ken Nishimura,Kana Morishita et al.
Emmanuel Osei Mensah et al.
Sendai virus (SeV)-based vectors are recognized as potential tools in gene therapy and regenerative medicine as they can express transgenes without chromosomal insertion. We previously reported that a replication-defective and persistent Se...
Engineering a high-performance isopropyl alcohol biosensor using a bio-nanocomposite multienzyme platform for real-time bioprocess monitoring [0.03%]
工程化高性能异丙醇生物传感器 实现实时生物工艺监控
Nisha Bhardwaj,Myeong-Eun Lee,Sung Ok Han
Nisha Bhardwaj
The spatial organization of multi-enzyme cascades offers a powerful strategy to overcome diffusion limitations and cofactor regeneration bottlenecks in industrial biocatalysis. In this study, a cellulosome-inspired complex was engineered to...
Deep learning revolutionizes protein research: Advances in structure prediction, functional annotation, and engineered design [0.03%]
深度学习革新蛋白质研究——结构预测、功能注释和工程设计中的进展
Jintong Zhang,Shengjie Wang,Le Gao et al.
Jintong Zhang et al.
Recent advances in deep learning have fundamentally transformed protein research by creating a synergistic cycle that connects structure prediction, functional annotation, and rational design. This review presents an integrative framework t...