Multi-level Integrated Engineering Enhances Neutral β-Glucanase Production in Komagataella phaffii [0.03%]
多级集成工程增强Komagataella phaffii中性β-葡聚糖酶的生产
Hai Xu,Xiaowei Shao,Yuzhe Peng et al.
Hai Xu et al.
Neutral β-1,3-glucanase (BgA) is a vital enzyme in the food industry, widely used to reduce viscosity in brewing mashes, improve dough rheology in baking, and clarify fruit juices. However, its industrial application is currently hindered ...
Synergistic antimicrobial and antiadhesive effects of benzazole derivatives and lactobacillus biosurfactants in vitro [0.03%]
苯并氮杂衍生物和乳酸菌生物表面活性剂的协同抗菌和防粘附作用：体外研究
Irany Mesquita Coelho Morais,Ihtisham Ul Haq,Andréa de Souza Monteiro et al.
Irany Mesquita Coelho Morais et al.
The investigation and development of novel antimicrobial compounds are central focuses of medical research. In this study, we evaluated benzimidazoles and benzothiazoles derivatives, and biosurfactants produced by Lactobacillus jensenii P6A...
Ethyl acetate extract of Lactococcus lactis KR-050L suppresses IL-6/STAT3 signaling in Hep3B cells and alleviates DSS-induced colitis in mice [0.03%]
乳酸链球菌KR-050L的乙酸乙酯萃取物通过抑制Hep3B细胞中的IL-6 / STAT3信号传导并在小鼠中缓解DSS诱导的结肠炎来发挥抗炎作用
Seon-Gyeong Bak,Nisansala Chandimali,Jaehoon Bae et al.
Seon-Gyeong Bak et al.
Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, is a chronic inflammatory condition characterized by persistent intestinal inflammation. Despite advances in therapeutic strategies, effective long-term man...
Homologous recombination-mediated assembly of multiple DNA fragments in Bacillus subtilis via protoplast transformation and inducible RecA expression [0.03%]
利用原生质体转化和可诱导的RecA表达在枯草芽孢杆菌中进行同源重组介导的DNA片段多重组装
Shinya Kaneko,Misako Nakahama,Hinata Hashimoto et al.
Shinya Kaneko et al.
We establish Bacillus subtilis (B. subtilis) as an alternative host for gap-repair cloning (GRC), a methodology that has been predominantly applied in Saccharomyces cerevisiae (S. cerevisiae), which requires recovery of assembled constructs...
Klaudia Žigová,Zuzana Marčeková,Alfonso Gautieri et al.
Klaudia Žigová et al.
The sudden COVID-19 pandemic highlights the importance of diagnostic assays in health security readiness. One persistent difficulty is the rapid molecular detection of pathogenic microorganisms and viruses. Taq DNA polymerase remains an ess...
Genomic insights into the biotechnological potential of Bacillus subtilis DW-2 for enhanced recovery of heavy oil [0.03%]
芽孢杆菌地衣芽孢杆菌DW-2的基因组学见解及其增强开采稠油的生物技术潜力
Yuqi Dang,Lu Ren,Weiguo Liu et al.
Yuqi Dang et al.
The extraction of heavy oil is challenged by high oil viscosity. Microbial enhanced oil recovery with indigenous bacteria offers a sustainable solution to improve the extraction efficiency of heavy oil. This study evaluated the biotechnolog...
Biocatalytic production of odour-active fatty aldehydes via inherent rice seedling α-dioxygenase activity [0.03%]
利用水稻幼苗内在的α- dioxygenase活性生物催化合成具有香气活性的脂肪醛类化合物
Jean-Philippe Kanter,Lars Milke,Christoph Harms et al.
Jean-Philippe Kanter et al.
Fatty aldehydes serve as key contributors to the odour profiles of many foods and are essential ingredients in numerous flavourings and fragrances. To meet the growing demand for sustainable and naturally sourced aldehydes, a biocatalytic m...
Effect of methanol, ethanol, DMSO and hexane on Chlorella vulgaris in the context of phytohormone delivery [0.03%]
甲醇、乙醇、DMSO和正己烷对植物激素递送的影响及这几种溶剂对小球藻生长和代谢的影响
Victor Pozzobon,Diego Lopez Salas,Camille Laury et al.
Victor Pozzobon et al.
Phytohormones have the potential to improve microalgae culture productivity. Still, not every phytohormone is water-soluble, and most have to be dissolved in organic solvents before being delivered to the cells. Unfortunately, most studies ...
Cyclic adenosine monophosphate (cAMP) signaling reprograms extracellular electron transfer of Shewanella oneidensis [0.03%]
环腺苷酸（cAMP）信号重编程产电菌Shewanella oneidensis的胞外电子传递路径
Lang Zhou,Xiaozhen Xu,Caiyi Li et al.
Lang Zhou et al.
Limited extracellular electron transfer (EET) efficiency constrains the electrocatalytic performance of Shewanella oneidensis MR-1. Although the c-type cytochrome (c-Cyts) network is essential for EET, its regulatory mechanisms remain incom...
Direct biosynthesis of medium-chain fatty acid methyl esters in a plant host using a rationally designed SABATH methyltransferase [0.03%]
利用理性设计的SABATH甲基转移酶在植物宿主中直接合成直链中链脂肪酸甲酯
Timothy A Chaffin,Nan Zhao,Hong Guo et al.
Timothy A Chaffin et al.
Fatty acid methyl esters (FAMEs) are recognized as promising biofuels. While engineering efforts have enabled FAME production in diverse microbes, no such work has been reported in plants. Here, we use an engineered SABATH methyltransferase...