Increased mRNA-lipid nanoparticle transfection per ng mRNA by concentrating cells [0.03%]
通过浓缩细胞提高每纳克mRNA的 mRNA-脂质纳米颗粒转染率
Alina Kunitskaya,Sunny P Chen,Chia Hao Ho et al.
Alina Kunitskaya et al.
The availability of T-cell therapies is limited in part due to their complex and costly biomanufacturing. Genetic engineering of T cells, such as to express chimeric antigen receptor (CAR), is commonly achieved using viral vectors, that are...
CHO endogenous weak promoters facilitating the development of high-yield cell lines [0.03%]
CHO细胞内源性弱启动子促进高产细胞系开发
Yuan Tian,Ya-Nan Zhou,Zhi-Ying Xiao et al.
Yuan Tian et al.
Efficient and stable transgene expression in Chinese hamster ovary (CHO) cells remains a critical challenge for biotherapeutic protein production. To advance the exploration and application of endogenous promoters in CHO cell line developme...
Effect of Mn²⁺ levels on caproic acid production during anaerobic fermentation using ethanol and butyric acid as co-substrates [0.03%]
Mn²⁺浓度对以乙醇和丁酸作为共底物厌氧发酵产己酸的影响研究
Longlong Liu,Yingmeng Shen,Sen Yang et al.
Longlong Liu et al.
As an enzyme cofactor, antioxidant, and stabilizer of cellular structures, Mn²⁺is essential for microbial growth, metabolism, and environmental adaptation. In this work, the impact of varying Mn²⁺ levels on caproic acid formation and mi...
Physiochemical and micro-morphological response for four bloom-forming algal strains under phosphite (P+III) stress and phosphate (P⁺ⱽ) recovery [0.03%]
磷（P~Ⅲ）胁迫及磷酸盐（P~Ⅴ）恢复下四种水华藻株的理化及微形态响应
Rongfei Zhang,Yufei Wang,Kun Wang et al.
Rongfei Zhang et al.
Phosphite (P⁺ᴵᴵᴵ), an emerging reduced phosphorus (P) species of environmental significance, exerts complex but poorly characterized effects on algal communities. In this study, we systematically evaluated interspecific variability in P...
Enabling methodology for high-throughput media screening in the context of multi-cell type co-cultures [0.03%]
多细胞共培养体系下的高通量培养基筛选技术及应用
Albane Carré,Céline Thomann,Inès Pedarré et al.
Albane Carré et al.
Recently, an increasing number of in vitro tissue models have been engineered, incorporating a growing variety of cell types more accurately reproduce native tissue architecture and function. However, optimisation of culture media to suppor...
Melatonin-Mediated Stress Signaling in Plants: Multilayered Networks and Translational Potential for Climate-Resilient Agriculture [0.03%]
植物中的褪黑素介导的应激信号转导：多层网络及其在气候适应型农业中应用的潜能
Madhan Krishnan,Shyamaladevi Babu,Alfred Solomon
Madhan Krishnan
Melatonin, initially identified as an animal hormone, is now well recognized as an essential bioactive molecule in plants, where it regulates a wide range of functions from growth and development to stress adaptation. Accumulating evidence ...
Leveraging mixed-mode filter during cell culture harvest to prevent antibody reduction [0.03%]
利用混合模式滤器进行细胞培养收获以防止抗体减少
Zhenshu Wang,Michael Hartmann,Geetanjali Pendyala et al.
Zhenshu Wang et al.
Disulfide bond reduction was observed for a monoclonal antibody (mAb1) produced in a Chinese Hamster Ovary (CHO) process, driven primarily by the thioredoxin (Trx)-thioredoxin reductase (TrxR) system present in harvested cell culture fluid ...
Production and accumulation of recombinant protein via vitellogenin transport pathway in Daphnia magna [0.03%]
利用溞卵黄素转运途径高效表达和积累重组蛋白质
Yusuke Tsuji,Fransiscus Jason Wiguna,Nikko Adhitama et al.
Yusuke Tsuji et al.
The demand for recombinant proteins is rising and various systems for protein production have been developed, including cultured cells, yeast, and bacteria. This study explores the use of Daphnia, a small crustacean, as a host for recombina...
Strengthening carbon concentrating mechanisms of ambient CO2 with OLA@KAUST-7 nanoparticles for producing microalgal biomass [0.03%]
用于在环境CO₂中增强碳浓缩机制的OLA@KAUST-7纳米颗粒以生产微藻生物量
Wanlin Liu,Ruhan Guo,Ying Liu et al.
Wanlin Liu et al.
In response to the mass transfer limitation of low concentration CO2 (approximately 400 ppm) in closed tubular photobioreactors aimed at improving indoor air quality, nanomaterials with excellent hydrolytic stability, OLA@KAUST-7, served as...
Synthetic biology-driven innovations in triple-negative breast cancer: Integrating engineering design with targeted therapeutics [0.03%]
合成生物学驱动的三阴性乳腺癌创新：工程设计与靶向治疗的整合
Negar Saliani,Mohammad Saeid Hejazi,Sepideh Zununi Vahed et al.
Negar Saliani et al.
Triple-negative breast cancer (TNBC) is a highly aggressive malignancy with limited therapeutic options and poor clinical outcomes due to the absence of hormone-responsive receptors. The advent of synthetic biology, which integrates molecul...