A cell-free platform for the high-efficiency synthesis and screening of PET hydrolase [0.03%]
一种高效合成和筛选PET水解酶的无细胞平台
Cuiyi Fu,Wenhui Shi,Jicheng Li et al.
Cuiyi Fu et al.
In this study, cell-free system (CFS) was employed to establish a platform for the high-efficiency synthesis and screening of PET hydrolase (PETase). This platform integrates the in vitro PETase expression using linear DNA templates, the in...
Casein-coated Fe3O4 nanozymes with enhanced peroxidase-like activity for synergistic doxorubicin delivery and catalytic anticancer therapy [0.03%]
具有增强类过氧化物酶活性的酪蛋白包被Fe3O4纳米酶用于协同多柔比星递送和催化抗癌治疗
Elahe Bayat,Samira Bagdeli,Asghar Taheri-Kafrani
Elahe Bayat
Cancer remains a complex and enduring challenge of the 21st century. Due to the limitations of current cancer treatments, the implementation of robust and innovative therapeutic strategies is crucial. The robust catalytic activity of nanozy...
Building a programmable protein factory: Recent advances in expression element engineering for Komagataella phaffii [0.03%]
构建可编程蛋白质工厂：毕赤酵母表达元件工程的最新进展
Yongmei Song,Zhe Zeng,Long Pan et al.
Yongmei Song et al.
The methylotrophic yeast Komagataella phaffii (formerly Pichia pastoris) has become one of the most widely adopted eukaryotic platforms for heterologous protein production, with more than 5000 recombinant proteins successfully expressed acr...
Activity-driven engineering of Baeyer-Villiger monooxygenase from Thermobifida fusca for the synthesis of methyl (2S)-5-chloro-2-hydroxy-1-oxo-2,3-dihydro-1H-indene-2-carboylate: a key precursor of (S)-indoxacarb [0.03%]
热解双歧杆菌拜尔-维林格单氧化酶的活性驱动工程化改造用于合成(S)-恶唑菌酮关键前体甲基(2S)-5-氯-2-羟基-1-氧代-2,3-二氢-1H-吲哚-2-甲酰酸酯
Xiafeng Lu,Yan Wu,Peibing Huang et al.
Xiafeng Lu et al.
The synthesis of key chiral intermediates via biocatalysis is of great importance in pharmaceutical manufacturing. In this study, we engineered a phenylacetone monooxygenase from Thermobifida fusca to enhance its catalytic activity for the ...
Dual-Functional Chitosan-Hyaluronic Acid Dialdehyde Nanoparticles for CD44-Targeted Bcl-2 siRNA Delivery and Photothermal Therapy in Bladder Cancer [0.03%]
用于靶向膀胱癌细胞CD44的Bcl-2siRNA递送和光热治疗的双功能壳聚糖-透明质酸二醛纳米粒子
Xin Liang,Yiming Sun,Yuanyuan Guo et al.
Xin Liang et al.
Bladder Cancer (BC) remains a major global health concern due to its high recurrence rate and limited availability of effective targeted therapies. Although gene therapy has emerged as a promising strategy, its clinical translation is hinde...
Morphological and Molecular Characterization of Mexican Hericium erinaceus Strains [0.03%]
墨西哥刺芹侧耳菌株的形态和分子特征分析
Mónica Amezcua,Alejandro Schcolnik-Cabrera,Elba Villegas
Mónica Amezcua
Hericium erinaceus is an edible and medicinal fungus recognized for its antimicrobial, antioxidant, anticancer, neuroprotective, and immunomodulatory properties. Despite its biological and biotechnological relevance, reports of this species...
Immobilization of lipase mediated by L-histidine-based ionic liquid as a bridge on magnetic COF and its application in isoamyl acetate synthesis [0.03%]
基于L-组氨酸的离子液体作为桥梁固定磁性COF负载脂酶及其合成异戊酰醋的应用研究
Renmin Liu,Tiantian Jia,Hanbing Song et al.
Renmin Liu et al.
This study proposes an innovative immobilized lipase construction strategy to address the low activity and poor stability limitations of current biocatalysts for flavor ester synthesis. L-histidine-based ionic liquid (IL) is covalently graf...
Adaptive laboratory evolution and rational engineering enabled xylose utilisation and xylan conversion in natural isolates of Saccharomyces cerevisiae [0.03%]
在酿酒酵母天然菌株中实现木糖利用和半纤维素转化的适应性试验进化和理性工程方法
Francois Kruger,Riaan den Haan
Francois Kruger
Second-generation biofuels produced from renewable lignocellulosic biomass (LCB) are attractive alternatives to environmentally damaging, non-renewable fossil fuels. A key challenge in converting LCB to bioethanol is the incomplete utilisat...
Integrating promoter engineering and BGC multiplication for enhancement of the new therapeutic agent chrysomycin A production [0.03%]
结合启动子工程和BGC倍增技术增强新药效剂金青霉素A的合成效率
Jiahui Wang,Yuanyuan Liu,Yunyun Lei et al.
Jiahui Wang et al.
Chrysomycin A (CA) is a promising drug candidate to treat multidrug-resistant Gram-positive bacterial infection with new modes of action. In order to improve CA production for new drug development, one marine-derived strain Streptomyces sp....
Enhancement of glutaminase production through protein design and host strain modification in Bacillus amyloliquefaciens [0.03%]
枯草芽孢杆菌谷氨酰胺酶的产量提高及其宿主菌改造和蛋白质设计
Dian Zou,Xiaoqi Ding,Pengfei Bao et al.
Dian Zou et al.
Glutaminase catalyzes the hydrolysis of γ-glutamyl groups to produce glutamic acid and ammonia, exhibiting broad-spectrum applications in food processing, fine chemicals, and pharmaceutical industries. However, its limited production effic...