Rational design of a bifunctional glycosyltransferase for enhanced substrate promiscuity and thermostability [0.03%]
通过理性设计获得底物通用性和热稳定性的双功能糖基转移酶
Dekai Liu,Ruifang Zhang,Peiqin Chen et al.
Dekai Liu et al.
Glycosylation of natural products significantly enhances their physicochemical properties, with glycosyltransferases (GTs) serving as the catalytic core of this biotransformation. Rational design of tailored GTs aligned with green chemistry...
Heavy ion beam irradiation-induced mutational profiles in Saccharomyces cerevisiae and their dependencies on dose and intracellular state inform an enhanced microbial breeding strategy [0.03%]
高能离子束辐照诱发酿酒酵母突变谱及其受剂量和细胞内状态影响的规律为改进微生物育种策略提供信息
Xiaopeng Guo,Xiuyue Xiao,Xuexia Dong et al.
Xiaopeng Guo et al.
Efficient microbial mutagenesis using heavy-ion beam (HIB) radiation is crucial for breeding. Here, Saccharomyces cerevisiae was irradiated with HIB across medium and high doses. Based on 65 randomly selected isolates, we systematically cha...
Multi-parameter process optimization for high yield AAV2 vector production using scale-down multi-parallel bioreactor systems and high-throughput analytical tools [0.03%]
基于规模缩小型多平行生物反应器系统及高效分析工具的高产量AAV2载体生产的多参数工艺优化
Nazgul Wagner,Jonas Austerjost,Julia Niemann
Nazgul Wagner
Adeno-associated virus (AAV) vectors are recognized as highly efficient carriers for genetic material. Among the various manufacturing platforms, transient production is the most established one for AAV production. Despite this, the transie...
Aluminum Phthalocyanine chloride Loaded Poly (lactic-co-glycolic acid) Nanoparticles based Photodynamic Therapy for Antibacterial and Anticancer Applications [0.03%]
载铝酞菁氯的聚乳酸-羟基乙酸共聚物纳米粒子光动力疗法在抗菌和抗肿瘤方面的应用
Anum Jamil,Aleena Zahid,Rafaqat Ali Khan et al.
Anum Jamil et al.
Photodynamic therapy (PDT) and antibacterial Photodynamic therapy (aPDT) have become a viable non-invasive treatment against cancer and bacterial infections. By using light-activated photosensitizers (PS), PDT effectively destroys cancer ce...
Semi-rational design of a thermostable O-glycosyltransferase from Glycyrrhiza uralensis for efficient conversion of protopanaxadiol [0.03%]
基于黄酮糖基转移酶结构的三七总皂苷双向转化酶的理性设计及应用研究
Jiajie Ding,Zhiying Ding,Yuan Liao et al.
Jiajie Ding et al.
Ginsenoside Rh2 (3-β-O-Glc-protopanaxadiol) and Rg3 (20-β-O-Glc-3-β-O-Glc-protopanaxadiol) are rare tetracyclic triterpenoids known for their significant medicinal properties, including antitumor and anti-inflammatory activities. The gly...
Soft-sensing strategy for real-time biomass and poly(3-hydroxybutyrate) estimation in methanotrophic cultures using dissolved oxygen and base addition signals [0.03%]
基于溶氧和碱液添加信号的甲基营养型微生物两相培养过程中的软传感策略
Seungyeon Cho,Kyutae Jung,Yoojin Shin et al.
Seungyeon Cho et al.
Real-time estimation of microbial biomass and product formation is crucial for effective monitoring and control of bioprocesses. In this study, we present a generalized soft-sensing algorithm that utilizes commonly available dissolved oxyge...
AbrB and ResD negatively regulate locillomycin synthesis in Bacillus velezensis Bs916 by binding to the flanking sequences of transcription start sites [0.03%]
AbrB和ResD通过结合转录起始位点附近的序列负向调控枯草芽孢杆菌Bs916产.locillomycin抗生素
Xiaohua Wang,HanWen Shen,Peng Zhang et al.
Xiaohua Wang et al.
Locillomycin, a cyclic lipopeptide produced by Bacillus velezensis Bs916, has been proven to have strong antimicrobial activity and low hemolytic activity. However, its low yield has become a significant obstacle for large-scale application...
Engineered BDH-NOx co-expression in Escherichia coli enables highly efficient in vivo cascade catalysis for the transformation of racemic epoxides to α-hydroxyketones [0.03%]
工程化的BDH-NOX共表达使重组大肠杆菌高效实现内源级联催化转化环氧化物为α-羟基酮
Bochun Hu,Xiaoqi Xi,Qianjin Hou et al.
Bochun Hu et al.
To eliminate the requirement for pyruvate supplementation and to avoid lactate accumulation, a multi-enzyme cascade was systematically restructured by replacing lactate dehydrogenase (LDH) with water-forming NADH oxidase (EfNOx). In this re...
Systematic engineering of cell wall for improving single cell protein (SCP) production [0.03%]
系统工程改造细胞壁以提高单细胞蛋白的产量
Linhai Xie,Shuo Tian,Zhehao Jin et al.
Linhai Xie et al.
As an emerging alternative protein, Single cell protein (SCP) has gained considerable traction in livestock feed applications. Furthermore, it presents substantial potential for replacing traditional dietary proteins for human consumption. ...
Cell specific nutrient feeding in perfusion mode enhances hMSC growth in stirred tank bioreactor process [0.03%]
基于细胞特异性营养需求的灌流培养促进反应器中人源间充质干细胞生长
Johanna Pechan,Kristina Engström,Nora Mac Key et al.
Johanna Pechan et al.
Human Mesenchymal Stromal Cells (hMSCs) are a safe option for allogeneic cell therapy across various diseases, but their manufacturing process requires improvement to broaden accessibility. In this study, a state-of-the-art planar multi-ves...