Couple action of some proteases from feather keratin-degrading bacterium B.licheniformis CP-16 [0.03%]
解羽菌枯草芽孢杆菌B.licheniformisCP-16中几种蛋白酶的联合作用研究
Shijin Luo,Deshan Wang,Kaige Yang et al.
Shijin Luo et al.
A keratin-degrading bacterium, Bacillus licheniformis CP-16 (B.licheniformis CP-16), isolated from feather waste, was found to produce three extracellular proteins with protease activity. Following mass spectrometry sequencing and BLAST ana...
Marine-derived Streptomyces sp. isolated from the Persian Gulf as a novel source of melanin [0.03%]
来自波斯湾的海洋链霉菌作为黑色素的新来源
Seyed Muhammad Hamid Malekpour,Moj Khaleghi,Alireza Akhtarpoor et al.
Seyed Muhammad Hamid Malekpour et al.
In this study, the structure and biological properties of melanin pigment produced by Streptomyces strain SEA5 isolated from Persian Gulf sediments were investigated. This strain was cultured in an enriched culture medium (L-tyrosine, meat ...
Corrigendum to "Structure-function analysis and exonuclease deletion yield an improved strand-displacing DNA polymerase from Aeribacillus pallidus for efficient recombinase polymerase amplification" [J. Biotechnol. 410 (2026) 321-330] [0.03%]
“来自Aeribacillus pallidus的高效重组酶聚合酶扩增用 strand-displacing DNA 聚合酶的结构功能分析和外切核酸酶删除”的勘误(文献编码：J. Biotechnol. 410 (2026) 321-330)
Koki Nishi,Eisuke Inoue,Yuto Murakami et al.
Koki Nishi et al.
Published Erratum
Journal of biotechnology. 2026 Jan 14:411:12. DOI:10.1016/j.jbiotec.2026.01.003 2026
Two industrial media reveal a mitochondrial disfunction in CHO cell cultures co-fed with glucose and lactic acid [0.03%]
两种工业培养基揭示了葡萄糖和乳酸共补料的CHO细胞培养中线粒体功能障碍
Keegan Orzechowski,Johanna Vappiani-Korben,Daniel C Sevin et al.
Keegan Orzechowski et al.
Metabolomics analyses of cell culture processes can provide valuable insight into cellular physiology that can be leveraged to develop more productive processes. In this work, we applied metabolomics to interrogate CHO cell behavior in two ...
CRISPR-Based precise methylation of specific FUT8 promoter regions allows isolation of CHO cells with a fine-tuned glycoprofile [0.03%]
基于CRISPR的FUT8启动子区域精准甲基化使具有精细调节型糖谱的CHO细胞得以富集分离
Víctor Jiménez Lancho,Klaus Leitner,Kitty Agarwal et al.
Víctor Jiménez Lancho et al.
A major advantage of producing therapeutic proteins in mammalian cells is their ability to tailor proteins with human-like posttranslational modifications such as glycosylation, which ultimately defines aspects like stability, protein foldi...
Structure-function analysis and exonuclease deletion yield an improved strand-displacing DNA polymerase from Aeribacillus pallidus for efficient recombinase polymerase amplification [0.03%]
来自Aeribacillus pallidus的高效重组酶聚合酶扩增改进型单链置换DNA聚合酶的结构功能分析及核酸外切酶缺失研究
Koki Nishi,Eisuke Inoue,Yuto Murakami et al.
Koki Nishi et al.
Isothermal nucleic-acid amplification relies on strand-displacing DNA polymerases that synthesize DNA while unwinding duplex templates. We previously identified two thermostable family-A polymerases, C1-Pol from Geobacillus zalihae and H1-P...
Engineered Lactococcus lactis expressing antimicrobial peptide HI: Enhanced survival and protection against ETEC in mice [0.03%]
工程化乳酸链球菌表达抗菌肽HI：在小鼠中增强了存活率并可预防ETEC感染
Mingyang Hu,Chongpeng Bi,Yuwen Li et al.
Mingyang Hu et al.
The rising prevalence of antibiotic resistance underscores the urgent need for alternative strategies to manage pathogenic bacteria. Engineered probiotics offer a promising platform for delivering antimicrobial peptides (AMPs); however, the...
Construction of an E. coli cell factory for ergothioneine through SAM-cycle enhancement and pathway reconstruction [0.03%]
通过SAM循环增强和途径重建构建工程生产麦角硫宁的大肠杆菌细胞工厂
Xiaoyu Zhang,Jiayue Guan,Yanqi Yang et al.
Xiaoyu Zhang et al.
Ergothioneine (EGT) is a rare sulfur-containing derivative of methionine with potent antioxidant, anti-inflammatory, and neuroprotective properties. Its unique bioactivities make it a promising ingredient for applications in functional food...
In vitro synthesis of β-aspartyl-basic amino acid dipeptides via a multi-enzyme cascade system with ATP regeneration [0.03%]
基于ATP再生的多酶级联体系体外合成β-天冬氨酰基碱性氨基酸二肽
Fei-Yan Cheng,Long-Wei Lou,Zong-Lin Li et al.
Fei-Yan Cheng et al.
Cyanophycin, also known as cyanophycin granule polypeptide (CGP), was a natural polyamide synthesized via a nonribosomal pathway from L-aspartic acid and L-arginine. Its derived dipeptide, β-Asp-Arg, holds potential applications in various...
Lyophilized chloroplasts as molecular capsules: A scalable platform for stable and functional human α-lactalbumin production [0.03%]
冻干叶绿体作为分子胶囊：稳定且具有功能的人类α-乳白蛋白生产的可扩展平台
Maryam Ehsasatvatan,Bahram Baghban Kohnehrouz
Maryam Ehsasatvatan
The growing world population and the increasing demand for proteins present significant challenges to food security. Advances in synthetic biology have enabled the development of transplastomic plants engineered to produce milk proteins, of...