Hairpin probe-based one-pot multiplex isothermal amplification combined with bifunctional G-quadruplex (IHP-GT) for the detection of alkaline phosphatase [0.03%]
基于发夹探针的一锅型多重等温扩增结合双功能G-四重螺旋结构（IHP-GT）检测碱性磷酸酶
Shuyu Zhu,Shiying Zhou,Liyuan Deng et al.
Shuyu Zhu et al.
Abnormal alkaline phosphatase (ALP) levels have been linked to breast cancer, prostate cancer, bone damage, gingivitis and abnormal liver function. Monitoring ALP levels is important for better diagnosis and treatment of these diseases. Det...
A dual-mode aptasensor based on rolling circle amplification enriched G-quadruplex for highly sensitive IFN-γ detection [0.03%]
一种基于滚环放大富集G-四聚体的双模式适配子传感器高效检测IFN-γ
Lianhui Zhao,Yingai Yin,Shuqi Xiao et al.
Lianhui Zhao et al.
Background: Aptasensors have been extensively utilized in target detection due to their advantages of high sensitivity and fast response. However, the reliability of the detection results of the single-mode aptasensor can...
Determination of bioequivalence between generic and reference drugs using laser-induced breakdown spectroscopy [0.03%]
激光诱导击穿光谱法测定仿制药与参比药的生物等效性
J Cardenas-Escudero,F Navarro-Villoslada,G Bellini et al.
J Cardenas-Escudero et al.
Background: In vitro bioequivalence studies are strictly limited to the comparison of dissolution performance to a reference drug. These studies are performed without considering the chemical similarity between the generi...
Microfluidic paper-based analytical devices for simple and nondestructive durian fruit maturity assessment [0.03%]
用于简单无损榴莲成熟度评估的微流体纸基分析装置
Jaruwan Mettakoonpitak,Atcha Chanthabun,Patcharaporn Hatsakhun et al.
Jaruwan Mettakoonpitak et al.
Accurately predicting durian maturity is a critically unresolved worldwide issue. Farmers currently determine durian ripeness based on their own observation and experience leading to inconsistencies in harvest timing. This reliance on human...
Stereoisomeric separation and chiral recognition mechanism study of star cyclodextrin polymer as the chiral stationary phase [0.03%]
手性星型环糊精聚合物的拆分机制研究
Hui Bai,Lei Chen
Hui Bai
Background: As the derivatives of cyclodextrin (CD), cyclodextrin polymers (CDPs) effectively increase the concentration of CD units and construct supramolecular structures with unique stereoselectivity by the structure d...
Per aqueous liquid chromatography of Radix hedysari polysaccharides and Au nanoparticles co-functionalized stationary phase and its application in the determination of iridoids and phenylethanols [0.03%]
基于槐糖多糖及Au纳米粒子协同功能化的固相的无水液相色谱法及其用于环烯醚萜和苯乙醇苷类成分分析研究
Tong Chen,Ye Shu,Guangsan Song et al.
Tong Chen et al.
Background: Hydrophilic Interaction Liquid Chromatography (HILIC) is an outstanding strategy for the challenging analysis of hydrophilic and polar components. Nevertheless, analysis under HILIC mode typically consumes 70%...
Phage LysSA163-CBD mediated specific recognition coupled with ATP bioluminescence for the sensitive detection of viable Staphylococcus aureus in food matrices [0.03%]
利用噬菌体LysSA163-CBD介导的特异性识别结合ATP生物发光法灵敏检测食品中活的大肠杆菌
Peng Guan,Ruining Li,Yifeng Ding et al.
Peng Guan et al.
Background: Staphylococcus aureus is a significant foodborne pathogen, commonly detected in milk and meat products. Conventional detection methods have limited sensitivity and specificity, which are time-consuming and sus...
Concentration and activation biresponsive strategy in one analysis system with simultaneous use of G4 structure-specific signal probe and enzyme-catalyzed reaction [0.03%]
集响应激活二响应策略在一个分析系统中同时使用G4结构特异性信号探针和酶催化反应
Yu-Shu Li,Chun-Feng Feng,Hao-Ran Chen et al.
Yu-Shu Li et al.
Background: Enzymes with critical effects on life systems are regulated by expression and activation to modulate life processes. However, further insights into enzyme functions and mechanisms in various physiological proc...
A supramolecular fluorescence probe that simultaneously responds to viscosity and G-quadruplex for autophagy detection [0.03%]
一种用于自噬检测的同时响应粘度和G-四链体的超分子荧光探针
Ruiyang Bai,Dawei Yang,Ranran Sun et al.
Ruiyang Bai et al.
Background: Autophagy, as an essential physiological process in eukaryotes, has been revealed to be closely related to aging and many major diseases. Real-time in situ imaging of autophagy processes in living cells is nec...
Isolation and quantification of human urinary exosomes using a Tween-20 elution solvent from polyester, capillary-channeled polymer fiber columns [0.03%]
使用聚酯和毛细通道聚合纤维柱结合吐温-20洗脱溶剂分离纯化人尿外泌体
Md Khalid Bin Islam,R Kenneth Marcus
Md Khalid Bin Islam
Background: Exosomes, a subset of extracellular vesicles (EVs), are a type of membrane-secreted vesicle essential for intercellular communication. There is a great deal of interest in developing methods to isolate and qua...