An off-target exonuclease activity in AsCpf1 undermines CRISPR diagnostics [0.03%]
AsCpf1的脱靶外切核酸酶活性使其在CRISPR诊断中表现不佳
Shixing Xue,He Sun,Xueyan Hou et al.
Shixing Xue et al.
The extensive utilization of CRISPR-Cas systems in molecular diagnostics stems from their crRNA-guided trans-cleavage capabilities. However, AsCpf1-based detection systems frequently exhibit unexplained sensitive variations. This research r...
Hierarchical MOFs with spatially ordered cascade enzymes for sensitive nitrite immunosensing [0.03%]
具有空间有序级联酶的层次化MOF用于灵敏检测亚硝酸根离子免疫传感器
Houru Li,Herui Wang,Mengxue Li et al.
Houru Li et al.
Enhancing the bioactivity and utilization efficiency of immobilized enzymes is essential for the construction of sensitive immunosensors. However, conventional approaches are often limited by random enzyme spatial distribution, which compro...
Loop-recognition dual-hairpin competitive probes enabling a dual-ratiometric electrochemical platform for specific SNP discrimination and logic gate construction [0.03%]
环识别双发夹竞争型探针使特定SNP区分的双重比值电化学生物传感平台及逻辑门构建成为可能
Wei Zhang,Xiang Tang,Yuhan Chen et al.
Wei Zhang et al.
Accurate discrimination of single nucleotide polymorphisms (SNPs) remains a fundamental challenge in nucleic acid biosensing due to the minimal thermodynamic differences between perfectly matched and single-base mismatched sequences. This i...
Tape-assisted nanoplasmonic biosensors for facile protein-on-chip visualization and multiplexed point-of-care diagnostics [0.03%]
用于芯片上蛋白质可视化和多重现场诊疗的磁带辅助纳米等离激元生物传感器
Lei Wang,Ying Yue,Yuan Chen et al.
Lei Wang et al.
Visual point-of-care testing (POCT) technologies convert biomolecular events into naked-eye readable signals. These systems offer rapid assay times, user-friendly operation, and eliminate the need for specialized instruments, making them pa...
Magnetically induced aggregation-assisted mesoporous polydopamine/single-atom nanozyme platform for dual-channel detection and two-stage synergistic eradication of pathogens [0.03%]
磁性诱导聚集增强的介孔聚多巴胺/单原子纳米酶平台用于病原体的双通道检测和两阶段协同根除
Lulu Cao,Yuwei Wu,Yuwei Ren et al.
Lulu Cao et al.
Contamination with foodborne pathogenic bacteria poses a severe threat to public health, necessitating the integration of detection with source sterilization technologies. Herein, we developed an integrated nanoplatform by combining an apta...
Photocrosslinked dextran hydrogel coatings with FAD-glucose dehydrogenase for continuous glucose monitoring in complex media: Key role of methacrylation degree on bioelectrocatalytic activity and sensor stability [0.03%]
用于复杂介质中连续葡萄糖监测的FAD-葡萄糖脱氢酶光交联葡聚糖水凝胶涂层：甲基丙烯酸化程度对生物电催化活性和传感器稳定性的重要作用
Bastien Darmau,Vladyslav Mishyn,Abir Elloumi et al.
Bastien Darmau et al.
Fungal flavin-dependent glucose dehydrogenase (FAD-GDH) is now widely preferred as an O2-insensitive alternative to glucose oxidase for 2nd generation blood glucose test strips. FAD-GDH bioelectrodes have potential for continuous glucose mo...
Nanoconfined biosensor for attomolar molecule detection and recycling via DNA strand displacement [0.03%]
基于DNA杂交链式反应的纳流控生物传感器用于超灵敏分子检测及循环放大
Yonghuan Chen,Xinru Yue,Minrui Long et al.
Yonghuan Chen et al.
Attomolar-level biomolecular detection remains challenging due to inefficient molecular collisions and weak signal transduction at ultralow concentrations. Here, we introduce a recyclable nanoconfined biosensing platform (ABA@NCBS) for aden...
Dual-scale bionic sensor with high stretchability for AI-enabled squatting motion monitoring [0.03%]
一种用于人工智能辅助下蹲动作监测的高拉伸性双尺度仿生传感器
Chuhan Zhang,Fujun Wang,Cunman Liang et al.
Chuhan Zhang et al.
Real-time monitoring of knee flexion during sports is critical for injury prevention, yet precise measurement remains challenging due to large flexion amplitudes and complex velocity-frequency characteristics. To address this challenge, we ...
DNA molecular subtraction controlled hybridization chain reaction for high contrast detecting and selective killing of cancer cells [0.03%]
基于DNA分子减法的杂交链反应高对比度检测与选择性杀伤肿瘤细胞
Fengrui Yang,Xiao-Rou Jiang,Lingling Lei et al.
Fengrui Yang et al.
miRNA-initiated hybridization chain reaction (HCR) has been explored for intracellular imaging and cancer immunomodulation. However, its specificity is often compromised because certain cancer-associated miRNAs are also expressed at low lev...
Neocuproine boosting catalytic activity of copper-based metal-organic frameworks for sensitive colorimetric immunosensor [0.03%]
硝基愈创草酚增强的铜基配位聚合物用于构建比色免疫传感器
Dan Li,Yueqi Liu,Yuchen Zhang et al.
Dan Li et al.
Metal-organic frameworks (MOFs) nanozymes represent promising alternatives to natural enzymes, but their catalytic activity and efficiency remain insufficient for practical immunosensing applications. Herein, a neocuproine (Nc)-boosted Cu-M...