Ilaria Chiaradia,Madeline A Lancaster
Ilaria Chiaradia
The complexity of human neurobiology is influenced by its heterogeneity, reflected in both cell type diversity as well as genetic variations between individuals. Neural organoids are 3D developing neural tissues designed to mimic developmen...
The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes [0.03%]
泛素连接酶RNF115参与受损溶酶体的清除
Sae Nakanaga,Toshiki Takahashi,Akiko Kuma et al.
Sae Nakanaga et al.
Lysosomes play a critical role in the quality control of cellular organelles. However, lysosomal membranes can be damaged under a variety of conditions, leading to the onset of various diseases. Damaged lysosomes are selectively cleared via...
Ida Calvi,Margherita Yayoi Turco
Ida Calvi
The placenta plays a vital role in supporting and nourishing the fetus throughout pregnancy, yet the mechanisms governing its development remain poorly understood. Recent advances in 3D human trophoblast organoid systems derived from both p...
Crystal structures of Klebsiella oxytoca ribitol dehydrogenase in complex with NAD+, d-allose, or d-allulose reveal insight into substrate recognition [0.03%]
肠杆菌科肺炎克雷伯氏菌核糖醇脱氢酶与NAD+，D-阿洛糖或D-全糖复合物的晶体结构为底物识别提供了新的见解
Hiromi Yoshida,Mayu Matsumoto,Naho Yamamoto et al.
Hiromi Yoshida et al.
Recombinant NAD+-dependent ribitol dehydrogenase derived from Klebsiella oxytoca (KoRdh) exhibits activity toward both ribitol and allitol. KoRdh catalyzes the NAD+-dependent oxidation of allitol to d-allulose and the NADH-dependent reducti...
Senescent cells acquire resistance to cystine deprivation-induced ferroptosis via the PPARα-PDK4-phosphorylated PDH axis [0.03%]
衰老细胞通过PPARα-PDK4-磷酸化PDH轴获得胱氨酸剥夺诱导的铁死亡抵抗性
Shiho Machii,Kazushi Morimoto,Pakawit Lerksaipheng et al.
Shiho Machii et al.
Cellular senescence, a state of irreversible cell cycle arrest, is implicated in age-related diseases. While it is well known that senescent cells resist apoptosis, studies on their resistance to ferroptosis are limited and not fully unders...
An isoform of 14-3-3 protein regulates transbilayer lipid movement at the plasma membrane [0.03%]
一个14-3-3蛋白异构体调节质膜上脂质的转层运动
Akiko Yamaji-Hasegawa,Akira Hattori,Masafumi Tsujimoto et al.
Akiko Yamaji-Hasegawa et al.
Exogenous phosphatidylserine (PS) is cytotoxic to Chinese hamster ovary (CHO) cells with a PS-resistant CHO cell mutant exhibiting impaired transbilayer movement of a fluorescent PS analog at the plasma membrane. Here, we demonstrate that b...
Genetically encoded lockdown of SERCA in the endoplasmic reticulum membrane arrests Ca2+ signaling through proximity-covalent crosslinking [0.03%]
基因编码的SERCA锁定作用于内质网膜并通过邻近共价交联抑制Ca2+信号转导
Yiying Li,Kozo Hamada
Yiying Li
Reversible conformational dynamics of membrane proteins are essential for intracellular signaling, but no method enables their irreversible arrest in living cells. Here, we developed a genetically encoded proximity-based lockdown enzyme der...
The E3 ubiquitin ligase UBR5 promotes ubiquitylation and degradation of the chromatin regulator ATAD2 [0.03%]
E3泛素连接酶UBR5促进染色质调节因子ATAD2的泛素化和降解
Rikuto Honda,Miyu Takao,Yoshino Akizuki et al.
Rikuto Honda et al.
UBR5 is a HECT-type E3 ubiquitin ligase that assembles K48-linked ubiquitin chains and generates K48-linked branched chains. UBR4 is another E3 that forms K48-linked chains through an atypical hemi-RING-like domain. To define substrates spe...
Asymmetry in ubiquitin binding by A20 reveals early recognition features of the ubiquitin code [0.03%]
A20的泛素结合不对称性揭示了泛素密码早期识别的特点
Erik Walinda,Julia Rose McCarthy-Page,Kazuhiro Iwai et al.
Erik Walinda et al.
Linear (Met1-linked) polyubiquitin chains play essential roles in NF-κB signaling, with readers such as A20 specifically recognizing these chains via specialized domains. Although structural data exist for the linear diubiquitin-A20 ZF7 co...
GDNF-RET signaling drives pulmonary neuroendocrine cell hyperplasia and allergic airway inflammation [0.03%]
GDNF-RET信号促进肺神经内分泌细胞增生和过敏性气道炎症
Tasuku Kawano,Erina Ike,Suguru Okada et al.
Tasuku Kawano et al.
Pulmonary neuroendocrine cell (PNEC) hyperplasia often occurs in lung diseases, including allergic asthma. We previously reported that PNEC-derived calcitonin gene-related peptide (CGRP) likely stimulates group 2 innate lymphoid cells (ILC2...