Terminal deoxynucleotidyl transferase (TdT) based template-free signal amplification for the detection of exosomes in MUC1-positive cells [0.03%]
基于末端脱氧核糖核酸转移酶（TdT）的无模板信号放大技术在MUC1阳性细胞源外泌体检测中的应用研究
Wenchang Fu,Kaige Yang,Mingyuan Wu et al.
Wenchang Fu et al.
The Mucin1 (MUC1) protein, involved in cytoprotective and signaling pathways, is abnormally elevated in various cancers, making it a key cancer indicator. Exosomes, which reflect the status of their originating cells, offer potential for ca...
Raman spectroscopy for monitoring free sulfhydryl formation during monoclonal antibody manufacturing [0.03%]
拉曼光谱监测单克隆抗体生产过程中的游离硫醇生成
Zhenshu Wang,Andrew Hsieh,Patricia Rose et al.
Zhenshu Wang et al.
During production, harvested cell culture fluid (HCCF) can degrade due to reductases breaking interchain disulfide bonds, forming low molecular weight (LMW) impurities that contain free sulfhydryl and high molecular weight (HMW) impurities ...
Evaluation of Jacalin lectin sorbents for the extraction of the human chorionic gonadotropin glycoforms prior to analysis by nano liquid chromatography-high resolution mass spectrometry [0.03%]
基于纳米液相色谱高分辨质谱分析人绒毛膜促性腺激素糖型的Jacalin凝集素吸附评价研究
Anastasia Goumenou,Christophe Chendo,Audrey Combès et al.
Anastasia Goumenou et al.
Human chorionic gonadotropin (hCG) is a dimeric, highly glycosylated hormone with a total of 4 N- and 4 O-glycosylation sites in its two subunits, hCGα and hCGβ. Recently, we developed a novel nano liquid chromatography coupled to high re...
A novel quantitative method for the determination of 10B-carrier boronophenylalanine in rat plasma by UHPLC-MS/MS and comparison with ICP-MS [0.03%]
一种用于测定大鼠血浆中~(10)B示踪剂硼苯丙氨酸的新方法及与ICP-MS法的比较研究
Yang Hu,Xin Jiang,Xiefeng Zhang et al.
Yang Hu et al.
L-Boronophenylalanine (BPA), a widely used 10B carrier for clinical boron neutron capture therapy (BNCT), was quantified in rat plasma through a simple, effective and stable ultra-high performance liquid chromatography-tandem mass spectrome...
Proteomic and network pharmacology analyses reveal S100A8 as the anti-inflammatory target of Yunpi Jiedu Tongluo Qushi Granule in the treatment of rheumatoid arthritis [0.03%]
蛋白组学和网络药理学分析揭示运脾解毒通络祛湿颗粒治疗类风湿关节炎的抗炎靶点为S100A8
Chenyi Yu,Honglv Jiang,Meijiao Wang et al.
Chenyi Yu et al.
Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease characterized by synovial inflammation. RA has a global prevalence between 0.5 % and 1 % although its pathogenesis is not completely understood. Chinese herbal medicine such...
Mass balance analysis for therapeutic peptides: Case studies, applications, and perspectives [0.03%]
治疗性肽的质控分析：案例研究、应用及展望
Evan M Hetrick,Brian W Pack,Chad N Wolfe et al.
Evan M Hetrick et al.
The concept of mass balance is discussed as it pertains to the pharmaceutical development of therapeutic peptides. Case studies are presented demonstrating how to perform a mass balance assessment on solid drug substance and solution drug p...
Plasma-based proteomic and metabolomic characterization of lung and lymph node metastases in cervical cancer patients [0.03%]
宫颈癌患者肺和淋巴结转移的血浆蛋白质组和代谢组特征分析
Yue Feng,Zijian Sun,Huan Zhang et al.
Yue Feng et al.
Metastasis is the leading cause of mortality in cervical cancer (CC), with a particular prevalence of lymph node and lung metastases. Patients with CC who have developed distant metastases typically face a poor prognosis, and there is a sca...
Characterization of adeno-associated virus capsid proteins using denaturing size-exclusion chromatography coupled with mass spectrometry [0.03%]
利用变性尺寸排阻色谱-质谱法表征腺相关病毒衣壳蛋白
Timothy N Tiambeng,Yuetian Yan,Shailin K Patel et al.
Timothy N Tiambeng et al.
Recombinant adeno-associated viruses (AAVs) are a highly effective platform for gene delivery for the treatment of many human diseases. Characterization of AAV viral protein attributes (VP), such as serotype identity, VP stoichiometry, and ...
Study on the mechanism of Xinmailong injection against chronic heart failure based on transcriptomics and proteomics [0.03%]
基于转录组和蛋白组的欣梅隆注射液治疗慢性心衰机制研究
Yanni Xue,Changling Lv,Lu Jin et al.
Yanni Xue et al.
Xinmailong (XML), a traditional Chinese medicine derived from Periplaneta americana, is commonly used in China to treat chronic heart failure (CHF). However, its pharmacological mechanism remains unclear. In our research, we employed Doxoru...
Separation of tolperisone and its degradation products by a dual cyclodextrin capillary electrophoresis system to study their potential role in allergic events [0.03%]
采用双环糊精毛细管电泳系统分离托吡酯及降解产物，研究其在过敏事件中的潜在作用
Péter P Lakatos,Zsuzsanna Ignáth,Orsolya Csernák et al.
Péter P Lakatos et al.
Tolperisone is a centrally acting muscle relaxant that has been used for the treatment of post-stroke spasticity and low back pain. Recently, the safety of tolperisone pharmaceutical products has been reassessed due to growing concerns over...