Diazonium-based derivatization for enhanced detection of phosphorylated metabolites by LC-MS in cells [0.03%]
基于重氮
Yikang Wang,Feifei Lin,Guozheng Zhu et al.
Yikang Wang et al.
Phosphorylated small molecule metabolites play crucial roles in physiological processes such as glycogen metabolism and inflammation regulation. However, their high polarity, structural similarity, poor chromatographic separation, and weak ...
Post-column denaturation-assisted hydrophobic interaction chromatography-mass spectrometry for rapid and in-depth characterization of positional isomers in cysteine-based antibody-drug conjugates [0.03%]
用于基于半胱氨酸的抗体-药物偶联物中位置异构体的快速和深入表征的柱后变性辅助疏水相互作用色谱-质谱法
Zhengqi Zhang,Anita P Liu,Hongxia Wang et al.
Zhengqi Zhang et al.
Antibody-drug conjugates (ADCs) represent a significant advancement in targeted cancer therapy, offering the potential to selectively deliver cytotoxic drugs to tumor cells while minimizing systemic toxicity. However, the structural complex...
Scaling analytical RP-HPLC to semi-preparative for fractionation and characterization of pegfilgrastim oxidized variants [0.03%]
用于pegfilgrastim氧化变异体分离和表征的分析型RP-HPLC到半制备型的放大方法
Haley Sutton,Teresa Youngberg,Christian Perez et al.
Haley Sutton et al.
Pegfilgrastim, a 40 kDa PEGylated form of recombinant human granulocyte colony-stimulating factor (rhG-CSF), is a biotherapeutic protein used to treat chemotherapy-induced neutropenia. To ensure the product is safe and effective, stringent ...
Evidence for the metabolic activation of higenamine to quinone methide and ortho-quinone metabolites in vitro and in vivo using liquid chromatography tandem mass spectrometry [0.03%]
高效液相串联质谱法在体内外研究HIG的醌甲基及邻位醌类代谢物激活反应证据
Hui Wang,Lihua Xin,Pengyi Hou et al.
Hui Wang et al.
Higenamine (HG), a naturally occurring benzyltetrahydroisoquinoline alkaloid, has been revealed a variety of biological activities and is extensively utilized in dietary supplements. Currently, HG is under investigation in phase I clinical ...
Analysis of solution-phase biomolecular interactions by liquid chromatography: General strategies and recent developments [0.03%]
液相色谱法分析溶液中生物大分子相互作用：通用策略及最新进展
David S Hage,Sadia Sharmeen,Kyungah Suh et al.
David S Hage et al.
The analysis of biomolecular interactions is important in characterizing and understanding many fundamental processes that occur in the body and biological systems. A variety of methods are available for studying the extent and rate of bind...
Metabolite profiling of Shenhua compound in rats and pharmacokinetics study of four bioactive compounds with liquid chromatography combined with electrospray ionization tandem mass spectrometry [0.03%]
神华胶囊大鼠药代动力学代谢组学研究及其中4个活性化合物的液相色谱-串联质谱法测定
Ze-Xuan Chen,Ling Li,Jie Tang et al.
Ze-Xuan Chen et al.
Shenhua compound, composed of ginseng, hawthorn and sophora flower, has been shown to improve hyperlipidemia. However, the main ingredients, their metabolic pathways in vivo, and pharmacokinetic characteristics. In this study, ultra-high-pe...
Quantification of serum daratumumab in multiple myeloma patients by LC-MS/MS, comparison with ELISA [0.03%]
液相色谱-串联质谱法测定多发性骨髓瘤患者血清达雷木单抗浓度及与酶联免疫吸附法比较
Weiqiang Li,Zhuoran Tian,Xiong Yu et al.
Weiqiang Li et al.
Daratumumab is a fully human immunoglobulin G1 monoclonal antibody employed for treating relapsed/refractory multiple myeloma and light-chain amyloidosis. Quantifying monoclonal antibodies in serum presents challenges due to interference fr...
Comparisons of the bioavailability of icariin, icariside II, and epimedin C in rats after oral administration of total flavonoids of Epimedium brevicornu Maxim. and its three formulations [0.03%]
口服淫羊藿短梗素总黄酮及其三种处方在大鼠体内的药动学及吸收利用度比较研究
Zhiyuan Ding,Xiuping Chen,Dongyun Tang et al.
Zhiyuan Ding et al.
The low bioavailability of insoluble flavonoids in the total flavonoids of Epimedium brevicornu Maxim. (TFE) severely hindered its clinical efficacy exertion. This research attempted to evaluate the promoting effects of pharmaceutical strat...
State-of-the-art chromatographic and mass spectrometric techniques in heparin structural analysis [0.03%]
高效液相色谱和质谱法在肝素结构分析中的应用进展
Yilan Ouyang,Siqi Yang,Wei Wang et al.
Yilan Ouyang et al.
Heparin is the most extensively used anticoagulant in clinical practice. It is a highly sulfated, linear polysaccharide composed of repeating disaccharide units. As a member of the glycosaminoglycan (GAG) family, heparin's complex structure...
Integrated untargeted metabolomics and bioactivity studies as new insights into the chemotaxonomy of Hura crepitans specimens from Peru and Sub-Saharan Africa [0.03%]
综合利用非靶向代谢组学和生物活性研究对秘鲁和东非的沙冬青进行化学分类的新见解
Elise Crossay,Valérie Cristofoli,Pedro Vásquez-Ocmín et al.
Elise Crossay et al.
Hura crepitans (Euphorbiaceae), is widespread in the Amazon rainforest and on plantations in sub-Saharan Africa. This tree produces an irritating milky latex rich in secondary metabolites, notably daphnane-type diterpenes and cerebrosides. ...