Ammonium adduct-triggered MS³ detection and post-column infusion in UPC²-MS³: A green strategy for sensitive tissue distribution study of methoxy polyethylene glycol with 6 subunits in zebrafish [0.03%]
基于UPC²-MS³的铵离子加合物触发的多级质谱检测及柱后切换技术：用于斑马鱼组织中具有6个单元的甲氧基聚乙二醇布地奈德敏感性分布研究的绿色策略
Shuang Feng,Yue Deng,Jiarui Zhang et al.
Shuang Feng et al.
Short-chain polyethylene glycol derivatives, such as methoxy polyethylene glycol with 6 subunits (M‑PEG₆‑OH), are extensively utilized excipients and building blocks, yet their in vivo biodistribution and associated safety profiles remai...
Optimizing LOD/LOQ and sample consumption during analytical ultracentrifugation to characterize AAV gene therapy vectors [0.03%]
优化分析超速离心法中的检测限和样本消耗以表征AAV基因治疗载体
Klaus Richter,Christine Wurm,Andrea Hawe et al.
Klaus Richter et al.
Production and development of adeno-associated viruses (AAV) for gene therapy applications requires sophisticated analytical methods to assess critical quality attributes of the product. A popular method providing relevant information on th...
Systemic defense against oxidative stress - The role of iron imbalance revealed by multiomics mechanisms leading to urosepsis [0.03%]
系统性氧化应激防御机制的研究——多组学揭示铁失衡导致尿源性脓毒症的分子机制
Paweł Wityk,Joanna Raczak-Gutknecht,Beata Krawczyk et al.
Paweł Wityk et al.
Urinary Tract Infections (UTIs) and urosepsis represent significant challenges in clinical medicine, necessitating a deeper understanding of the molecular intricacies underlying these conditions. In this study, we employed a multi-omics app...
Mechanism of Qibai Pingfei Capsule in ameliorating COPD-associated inflammation via the ABCA3/PPARγ/NF-κB pathway: A serum-targeted metabolomics and lipidomics study [0.03%]
基于血清目标组学的百平肺菲胶囊改善慢性阻塞性肺疾病相关炎症的作用机制：ABCA3/PPARγ/NF-κB信号通路调控研究
Siyu Tang,Mengxiang Liu,Yating Gao et al.
Siyu Tang et al.
Chronic obstructive pulmonary disease (COPD) is a global health issue driven by chronic inflammation. Although Qibai Pingfei Capsule (QBPF) shows clinical efficacy in COPD, its multi-component, multi-target nature complicates mechanistic st...
Pharmacokinetics and qualitative metabolite profiling of major alkaloids in rats after acupoint administration of Xiatianwu Injection [0.03%]
穴位注射下田乌注射液后鼠源主要生物碱药代动力学及代谢产物动态监测研究
Hengbin Zhang,Yuyang Zhao,Hui Liu et al.
Hengbin Zhang et al.
Xiatianwu (XTW) Injection, derived from Corydalis Decumbentis Rhizoma, is clinically utilized to promote circulation and alleviate pain. However, its effective components and pharmacokinetic profile have yet to be elucidated. In the present...
Integrated LC-HRMS/MS-based metabolomics and in vitro and in vivo bioassays for comparative evaluation of two ethnomedicinal Paris species and their official counterparts [0.03%]
基于整合的LC-HRMS / MS代谢组学和体内外生物测定法比较评估两种民族药Paris及其官方品种
Ting Yu,Yuan Sun,Fang-Ning Qiu et al.
Ting Yu et al.
Paris species, known as "Chonglou" in traditional Chinese medicine, have been used clinically for millennia. Nevertheless, the variation patterns of their chemical constituents and biological activities among different species are still poo...
Perturbation of the quaternary state of insulin aspart formulation after prolonged storage in a closed-vial, real-world setting [0.03%]
一种人胰岛素类似物门冬胰岛素在室温密闭安瓿瓶中长期储存后的四聚体结构变动分析
Ashish Kawale,Daniel Malmodin,Gun Forsander et al.
Ashish Kawale et al.
People with diabetes from many developing countries find it challenging to follow manufacturers' guidelines regarding strict storage conditions for insulin. What happens to insulin formulations exposed to real-world scenarios is important t...
An ultrasensitive chemiluminescence immunoassay for the detection of thyroid stimulating hormone based on antibody charge distribution-guided directional conjugation on magnetic beads [0.03%]
基于抗体电荷分布导向磁珠上定向偶联的超灵敏化学发光免疫分析法检测促甲状腺激素
Zhifang Wan,Zhiwei Lu,Pei Jiang et al.
Zhifang Wan et al.
To address the insufficient sensitivity (0.01 μIU/mL) of the currently widely used "third-generation" thyroid-stimulating hormone (TSH) detection reagent, we developed a "fourth-generation" ultrasensitive chemiluminescence immunoassay (CLI...
Investigating the pharmacokinetics and conjugate metabolism of potential hepatotoxic prenylflavonoids in Epimedium extract using LC-MS/MS [0.03%]
基于LC-MS/MS的黄毛豆提取物潜在肝毒性倍半萜黄酮类成分的药代动力学及结合代谢研究
Xiyi Peng,Lin Zhang,Shengyao Gao et al.
Xiyi Peng et al.
This study aimed to investigate the pharmacokinetics and conjugate metabolism of four potentially hepatotoxic prenylflavonoids-sagittatoside A, icariside I, baohuoside I, and icaritin-from Epimedium extract. A rapid and sensitive liquid chr...
Identification of anticancer and cardioprotective quality markers in Chuanxiong Rhizoma using a bioassay driven effect-constituent index approach [0.03%]
基于效应标志物的川芎抗肿瘤及心血管保护质量标志物识别研究
Jiahui Yang,Xintong Liu,Linlin Fan et al.
Jiahui Yang et al.
Ligusticum Chuanxiong (Chuanxiong Rhizoma) is used in Traditional Chinese medicine (TCM) and has cardioprotective effects. Trastuzumab improves outcomes in HER2-positive breast cancer, but acquired resistance and cardiotoxicity limit its ut...