MicroLC-HRMS selective quantification of a biotherapeutic fusion protein in plasma and cerebrospinal fluid of patients with Lafora disease using a linker-containing signature peptide [0.03%]
使用连接器含特色肽的选择性量化患者郎飞氏病血浆和脑脊液中的生物治疗融合蛋白的MicroLC-HRMS方法
Erika Esposito,Alice Caravelli,Lorenzo Muccioli et al.
Erika Esposito et al.
Although mass spectrometry-based approaches are increasingly used for protein biotherapeutic bioanalysis, validated workflows remain limited for fusion proteins across multiple clinical matrices, including cerebrospinal fluid (CSF). We deve...
Investigation of the on-column oxidative formation of impurity D during amlodipine impurities profiling by UHPLC [0.03%]
超高压液相色谱法测定氨氯地平杂质图谱时杂质D的柱内生成机制研究
Michal Douša,Stanislav Kalášek
Michal Douša
The determination of related substances in amlodipine salts is a critical aspect of pharmaceutical quality control. This study investigated the unexpected formation of impurity D, a substituted pyridine derivative, observed as an on-column ...
Systematic development and optimization of a cIEF method for the charge variants analysis of moderately basic to basic monoclonal antibodies using the Analytical Quality by Design strategy [0.03%]
基于Analytical Quality by Design策略的单克隆抗体电荷异构体cIEF方法的系统开发与优化
Lucrezia Floris,Benedetta Pasquini,Serena Orlandini et al.
Lucrezia Floris et al.
This study proposes a Capillary IsoElectric Focusing Analytical Procedure Platform for the charge heterogeneity characterization of monoclonal antibodies. The Analytical Quality by Design approach was implemented to define a robust Method O...
Determination of five antihypertensives in dried capillary plasma spots by LC-MS/MS: A novel method for monitoring pharmacotherapy adherence [0.03%]
LC-MS/MS测定干血点中五种抗高血压药物的含量：一种监测药物治疗依从性的新方法
Debora Renata Macali Oliveira,Fernanda Fischer Costa,Juliana Henrique Duarte et al.
Debora Renata Macali Oliveira et al.
Adherence to hypertension treatment is crucial for therapeutic success, as noncompliance adversely affects quality of life and healthcare costs. Assessing adherence directly through plasma drug concentrations is particularly effective for p...
Analytical quality by design-based development and validation of a robust impurity analysis method for albumin-bound paclitaxel nanoparticles [0.03%]
基于质量设计的用于紫杉醇白蛋白纳米粒产品的杂质分析方法的研究与验证
Hye Jeong,Myung-Chul Gil,Young Ho Cho et al.
Hye Jeong et al.
Analytical Quality by Design (AQbD) was applied to develop a robust impurity (IMP) analysis method for albumin-bound paclitaxel nanoparticles (Nab-PTX). Preliminary studies revealed that the USP method could induce epimerization, increasing...
Interference of target protein translation in multivalent mRNA vaccine in cell-based assay [0.03%]
基于细胞的试验中的多价mRNA疫苗干扰靶蛋白翻译
Alyssa Q Stiving,Geethanjali Dornadula,Benjamin W Roose et al.
Alyssa Q Stiving et al.
Messenger RNA (mRNA) vaccines are highly effective against infectious diseases, but viral diversity has underscored the necessity for multivalent and combination vaccines. We hypothesize there could be interference of target protein transla...
AI-assisted integrative framework combining microarray data analysis and cerebrospinal fluid pharmacology for revealing molecular mechanism in Alzheimer's disease: A case study of Psoraleae Fructus [0.03%]
结合基因芯片数据分析和脑脊液药理的AI辅助集成框架：以补骨脂果实为例探究阿尔茨海默病的分子机制
Xueyan Li,Jinchai Qi,Fulu Pan et al.
Xueyan Li et al.
Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by a gradual decline in cognitive function, with a complex pathogenesis involving multiple targets and signaling pathways, and current therapeutic strategies...
Comparison of feature-based molecular networking strategies for untargeted metabolite identification in endophytic fungal samples from Lespedeza bicolor Turcz [0.03%]
基于特征的分子网络策略在Lespedeza bicolor Turcz内生真菌样品非目标代谢物鉴定中的比较研究
Yuhang Liu,Qixian Zheng,Qing Liu et al.
Yuhang Liu et al.
Feature-Based Molecular Networking (FBMN) is a robust strategy for the structural elucidation of natural products; however, inconsistent workflows across software platforms and ionization modes hinder its standardized application. This stud...
Systematic characterization of active components and therapeutic mechanisms of Chaihu Guizhi Ganjiang Decoction in metabolic dysfunction-associated steatohepatitis [0.03%]
柴胡桂枝干姜汤活性成分及代谢功能障碍相关性非酒精性脂肪肝病治疗机制的系统研究
Hao Wu,Tengteng Wang,Shengnan Cai et al.
Hao Wu et al.
Chaihu Guizhi Ganjiang Decoction (CGGD) is a clinically proven prescription effective against metabolic dysfunction-associated steatohepatitis (MASH). However, its chemical constituents with the lipid-lowering and anti-inflammatory bioactiv...
Quantification of PEG2000-DMG and TS-202, an ionic lipid, in rabbit plasma by LC-MS/MS: Application to complement C5 siRNA lipid nanoparticles [0.03%]
LC-MS/MS法测定兔血浆中PEG2000-DMG和TS-202的含量及其在补体C5 siRNA脂质纳米颗粒中的应用
Yuji Mano,Koichiro Hotta
Yuji Mano
A simple and robust assay was developed for the quantification of PEG2000-DMG and TS-202, key components of the lipid nanoparticle (LNP) formulation of E8001-LNP, which encapsulates small-interfering RNA targeting complement C5, in rabbit p...