Stable expression of green fluorescent protein and targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis with the WR99210/dhfr selection system [0.03%]
伯氏疏螺旋体WR99210/dhfr筛选系统中绿色荧光蛋白稳定表达及Babesia bovis硫氧还蛋白过氧化物酶-1基因靶向敲除
Masahito Asada,Miho Tanaka,Yasuyuki Goto et al.
Masahito Asada et al.
We have achieved stable expression of green fluorescent protein (GFP) in Babesia bovis by using the WR99210/human dihydrofolate reductase (DHFR) gene selection system. A GFP-expression plasmid with a dhfr expression cassette (DHFR-gfp) was ...
A new molecular approach for cidal vs static antimalarial determination by quantifying mRNA levels [0.03%]
一种通过量化mRNA水平判断杀菌与抑菌型抗疟药的新分子方法
Noemí Bahamontes-Rosa,Ane Rodríguez-Alejandre,Rubén González-del-Rio et al.
Noemí Bahamontes-Rosa et al.
In order to maximise compliance, the future antimalarial treatment should ideally require just a single-dose administration. This, in turn, demands new fast-acting effective drugs. Currently, methods to measure the in vitro killing rate of ...
Inga Siden-Kiamos,Christos Louis,Kai Matuschewski
Inga Siden-Kiamos
Extracellular stages of apicomplexan parasites utilize their own actin myosin motor machinery for gliding locomotion, penetration of cell barriers, and host cell invasion. Thus far, filamentous actin could not be visualized by standard micr...
Mohammed Tarique,Akash Tripathi Satsangi,Moaz Ahmad et al.
Mohammed Tarique et al.
Malaria is one of the most important infectious diseases in many regions around the world including India. Plasmodium falciparum is the cause of most lethal form of malaria while Plasmodium vivax is the major cause outside Africa. Regardles...
Jason L Weirather,Mary E Wilson,John E Donelson
Jason L Weirather
The protozoan parasite Trypanosoma brucei switches its variant surface glycoprotein (VSG) to subvert its mammalian hosts' immune responses. The T. brucei genome contains as many as 1600 VSG genes (VSGs), but most are silent noncoding pseudo...
Characterization of acyl carrier protein and LytB in Babesia bovis apicoplast [0.03%]
贝氏等孢球虫.apicoplast.酰基载体蛋白和LytB的性质研究
Marina C Caballero,Monica J Pedroni,Guy H Palmer et al.
Marina C Caballero et al.
The apicoplast is a highly specialized organelle that mediates required functions in the growth and replication of apicomplexan parasites. Despite structural conservation of the apicoplast among different parasite genera and species, there ...
Glu-108 is essential for subunit assembly and dimer stability of D-phosphoglycerate dehydrogenase from Entamoeba histolytica [0.03%]
Entamoeba.histolytica中天冬氨酸磷酸激酶的二聚稳定性及亚基装配需要Glu-108位点
Vibhor Mishra,Ashutosh Kumar,Vahab Ali et al.
Vibhor Mishra et al.
D-phosphoglycerate dehydrogenase catalyses the first step of phosphorylated serine biosynthesis pathway by oxidizing 3-phosphoglycerate, a glycolysis intermediate into phosphohydroxsy pyruvate. For Entamoeba histolytica this pathway is an i...
Excretory/secretory antigens from Dirofilaria immitis adult worms interact with the host fibrinolytic system involving the vascular endothelium [0.03%]
心丝形线虫成虫的排泄/分泌抗原与宿主纤溶系统的血管内皮相互作用
Javier González-Miguel,Rodrigo Morchón,Isabel Mellado et al.
Javier González-Miguel et al.
Dirofilaria immitis is the causative agent of canine and feline heartworm disease. The parasite can survive for long periods of time (7 years or more) in the circulatory system of immunocompetent reservoirs, producing usually a chronic infl...
Role of trypanosomatid's arginase in polyamine biosynthesis and pathogenesis [0.03%]
赖氨糖酶在多胺生物合成和发病中的作用
Rafael Balaña-Fouce,Estefanía Calvo-Álvarez,Raquel Álvarez-Velilla et al.
Rafael Balaña-Fouce et al.
L-Arginine is one of the precursor amino acids of polyamine biosynthesis in most living organisms including Leishmania parasites. L-Arginine is enzymatically hydrolyzed by arginase producing L-ornithine and urea. In Leishmania spp. and othe...
The molecular epidemiology of parasite infections: tools and applications [0.03%]
寄生虫感染的分子流行病学：工具和应用
A J Lymbery,R C A Thompson
A J Lymbery
Molecular epidemiology, broadly defined, is the application of molecular genetic techniques to the dynamics of disease in a population. In this review, we briefly describe molecular and analytical tools available for molecular epidemiologic...