Kinetic analyses and inhibition studies reveal novel features in peptide deformylase 1 from Trypanosoma cruzi [0.03%]
动力学分析及抑制研究揭示了来自裂殖体原虫的肽脱甲酰基酶1的新特征
Carlos A Rodrígues-Poveda,Guiomar Pérez-Moreno,Antonio E Vidal et al.
Carlos A Rodrígues-Poveda et al.
In eubacteria and eukaryotic organelles N-terminal methionine excision requires the sequential action of two activities, a peptide deformylase (PDF), which systematically removes the N-formyl group present on all nascent polypeptides and me...
Longistatin is an unconventional serine protease and induces protective immunity against tick infestation [0.03%]
长刺蛋白是一种非常规丝氨酸蛋白酶 并可在蜱虫侵袭时诱导保护性免疫反应
Anisuzzaman,M Khyrul Islam,M Abdul Alim et al.
Anisuzzaman et al.
Classical serine proteases use the conserved Ser/His/Asp catalytic triad to hydrolyze substrates. Here, we show that longistatin, a salivary gland protein with two EF-hand domains from the vector tick Haemaphysalis longicornis, does not hav...
Purification, molecular characterization and gene expression analysis of an aspartic protease (Sc-ASP113) from the nematode Steinernema carpocapsae during the parasitic stage [0.03%]
Steinernema carpocapsae 原矛尾线虫寄生阶段天冬氨酸蛋白酶Sc-ASP113的纯化、分子鉴定及基因表达分析
Natesan Balasubramanian,Duarte Toubarro,Gisela Nascimento et al.
Natesan Balasubramanian et al.
Steinernema carpocapsae is an insect parasitic nematode associated with the bacterium Xenorhabdus nematophila. During invasion, this nematode is able to express many proteases, including aspartic proteases. Genes encoding these aspartic pro...
Joana M Santos,Arnault Graindorge,Dominique Soldati-Favre
Joana M Santos
The rhomboid-like proteins constitute a large family of intramembrane serine proteases that are present in all branches of life. First studied in Drosophila, these enzymes catalyse the release of the active forms of proteins from the membra...
Comparative biochemical and functional properties of two leucine aminopeptidases of Clonorchis sinensis [0.03%]
中国肝吸虫两种亮氨酸氨基肽酶的生化及功能特性比较
Jung-Mi Kang,Hye-Lim Ju,Jung-Won Ju et al.
Jung-Mi Kang et al.
Leucine aminopeptidases (LAP; EC 3.4.11.1) are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes th...
A GFP-actin reporter line to explore microfilament dynamics across the malaria parasite lifecycle [0.03%]
报告疟原虫生命周期中微丝动态的GFP-肌动蛋白系
Fiona Angrisano,Michael J Delves,Angelika Sturm et al.
Fiona Angrisano et al.
Malaria parasite motility relies on an internal parasite actomyosin motor that, when linked to the host cell substrate, propels motile zoites forward. Despite their key role in this process, attempts to visualize actin microfilaments (F-act...
Trypanosoma brucei brucei oligopeptidase B null mutants display increased prolyl oligopeptidase-like activity [0.03%]
缺少oligopeptidase B的特布雷亚无鞭毛体显示出增强的prolyl寡肽酶样活性
Richard T Kangethe,Alain F V Boulangé,Virginie Coustou et al.
Richard T Kangethe et al.
African trypanosomosis is a parasitic disease in man and animals caused by protozoan parasites of the genus Trypanosoma. Nagana, the cattle form of the disease, is caused by Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei b...
The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis [0.03%]
中国肝吸虫抗原蛋白的鉴定：14-3-3蛋白质和丙酰辅酶A羧酸酶
Myoung-Ro Lee,Yu-Jung Kim,Dae-Won Kim et al.
Myoung-Ro Lee et al.
Clonorchis sinensis, the causative agent of clonorchiasis, is widespread in East and Southeast Asia, including China, Vietnam and the Republic of Korea. We identified antigenic proteins from adult C. sinensis liver flukes using immunoproteo...
Cytochrome P450 in fluke Opisthorchis felineus: identification and characterization [0.03%]
猫斑氏肝吸虫（Opisthorchis felineus）中的细胞色素P450：鉴定与特性分析
Maria Y Pakharukova,Nikita I Ershov,Elena V Vorontsova et al.
Maria Y Pakharukova et al.
Infection with the human liver fluke Opisthorchis felineus is a serious public health problem in Russia and other Eastern Europe countries. The aim of this work was to identify and sequence cytochrome P450 mRNA from O. felineus and to analy...
The crystal structure of Leishmania major N(5),N(10)-methylenetetrahydrofolate dehydrogenase/cyclohydrolase and assessment of a potential drug target [0.03%]
利什曼原虫N(5)，N(10)-亚甲基四氢叶酸脱氢酶/环水解酶的晶体结构及其作为潜在药物靶标的评估研究
Thomas C Eadsforth,Scott Cameron,William N Hunter
Thomas C Eadsforth
Three enzyme activities in the protozoan Leishmania major, namely N(5),N(10)-methylenetetrahydrofolate dehydrogenase/N(5),N(10)-methenyltetrahydrofolate cyclohydrolase (DHCH) and N(10)-formyltetrahydrofolate ligase (FTL) produce the essenti...