The complete mitochondrial genome sequence of the filarial nematode Wuchereria bancrofti from three geographic isolates provides evidence of complex demographic history [0.03%]
采自三个地理分离群体的班氏吴策线虫完整线粒体基因组序列揭示其复杂的种群历史
Akshaya Ramesh,Scott T Small,Zachary A Kloos et al.
Akshaya Ramesh et al.
Mitochondrial (mt) genome sequences have enabled comparison of population genetics and evolution for numerous free-living and parasitic nematodes. Here we define the complete mt genome of Wuchereria bancrofti through analysis of isolates fr...
Establishment of a transient transfection system and expression of firefly luciferase in Entamoeba invadens [0.03%]
侵袭艾美尔阿米巴中萤火虫荧光素酶的表达及瞬时转染体系的建立
Nishant Singh,Sandeep Ojha,Alok Bhattacharya et al.
Nishant Singh et al.
Entamoeba invadens is used as a model system to study trophozoite to cyst differentiation since Entamoeba histolytica, the causative agent of amoebiasis cannot encyst in culture. However, a system for introduction of cloned genes in E. inva...
Expression and characterization of catalytic domain of Plasmodium falciparum subtilisin-like protease 3 [0.03%]
恶性疟原虫半胱氨酸蛋白酶fcp-2_like的克隆、表达及活性检测
Asrar Alam,Raj K Bhatnagar,Virander S Chauhan
Asrar Alam
PfSUB3 is the third subtilisin-like protease annotated in Plasmodium genome database "PlasmoDB". The other two members, PfSUB1 and PfSUB2 have been implicated in merozoite egress and invasion in asexual blood stages. In this study, we recom...
Identification of a specific region of Plasmodium falciparum EBL-1 that binds to host receptor glycophorin B and inhibits merozoite invasion in human red blood cells [0.03%]
鉴定出一个特定的区域：恶性疟原虫EBL-1结合宿主受体糖蛋白B并抑制人红细胞内期寄生虫入侵
Xuerong Li,Marina Marinkovic,Crystal Russo et al.
Xuerong Li et al.
The malaria parasite Plasmodium falciparum invades human erythrocytes through multiple pathways utilizing several ligand-receptor interactions. These interactions are broadly classified in two groups according to their dependency on sialic ...
Excretory/secretory products from in vitro-cultured Echinococcus granulosus protoscoleces [0.03%]
从试管培养的细粒棘球蚴原头节中发现的排泄/分泌产物
Veridiana G Virginio,Karina M Monteiro,Fernanda Drumond et al.
Veridiana G Virginio et al.
Cystic hydatid disease (CHD) is caused by infection with Echinococcus granulosus metacestodes and affects humans and livestock. Proteins secreted or excreted by protoscoleces, pre-adult worms found in the metacestode, are thought to play fu...
Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry [0.03%]
恶性疟原虫代谢性寡糖工程技术使直接以质谱法分析糖脂成为可能
Tamara Piñero,Valnice J Peres,Alejandro Katzin et al.
Tamara Piñero et al.
A recent addition to the arsenal of tools for glycome analysis is the use of metabolic labels that allow covalent tagging of glycans with imaging probes. In this work we show that N-azidoglucosamine was successfully incorporated into glycol...
Genome-wide identification and characterization of a panel of house-keeping genes in Schistosoma japonicum [0.03%]
日本血吸虫管家基因的全基因组范围鉴定与特征分析
Shuai Liu,Pengfei Cai,Nan Hou et al.
Shuai Liu et al.
Quantitative real-time polymerase chain reaction (qPCR), as one of the most sensitive and precise gene expression analysis methods, is frequently used to validate data obtained in high-through-put assays. qPCR requires reference genes with ...
Characterization and differential expression of a ferritin protein from Fasciola hepatica [0.03%]
肝片吸虫铁蛋白的特征及差异表达
Kimberly Cabán-Hernández,José F Gaudier,Ana M Espino
Kimberly Cabán-Hernández
Ferritins are proteins that play a central role in maintaining intracellular iron balance. A cDNA clone of Fasciola hepatica (687 bp long) encoding a putative 228-amino acid polypeptide (FhFtn-1) homologous with ferritins of vertebrates and...
Molecular and functional characterization of the ceramide synthase from Trypanosoma cruzi [0.03%]
克氏锥虫丝氨酸 palmitoyl-CoA 胺甲基转移酶的分子和功能表征
Juliana M Figueiredo,Deivid C Rodrigues,Rafael C M C Silva et al.
Juliana M Figueiredo et al.
In this study, we characterized ceramide synthase (CerS) of the protozoan parasite Trypanosoma cruzi at the molecular and functional levels. TcCerS activity was detected initially in a cell-free system using the microsomal fraction of epima...
Role of expression site switching in the development of resistance to human Trypanosome Lytic Factor-1 in Trypanosoma brucei brucei [0.03%]
表达位点转换在克隆布氏泰勒虫人类溶锥因子1耐药性发展中的作用研究
Rudo Kieft,Natalie A Stephens,Paul Capewell et al.
Rudo Kieft et al.
Human high-density lipoproteins (HDLs) play an important role in human innate immunity to infection by African trypanosomes with a minor subclass, Trypanosome Lytic Factor-1 (TLF-1), displaying highly selective cytotoxicity to the veterinar...