Acute and persistent infection by a transfected Mo7 strain of Babesia bovis [0.03%]
莫氏牛东毕吸虫感染的急性与慢性阶段
Carlos E Suarez,Jacob M Laughery,David A Schneider et al.
Carlos E Suarez et al.
A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in a...
Toxoplasma ISP4 is a central IMC sub-compartment protein whose localization depends on palmitoylation but not myristoylation [0.03%]
乙酰化对于内膜复合体中心亚隔室蛋白ISP4在胞质分裂环中的定位是必需的
Connie Fung,Josh R Beck,Seth D Robertson et al.
Connie Fung et al.
Apicomplexan parasites utilize a peripheral membrane system called the inner membrane complex (IMC) to facilitate host cell invasion and parasite replication. We recently identified a novel family of Toxoplasma IMC Sub-compartment Proteins ...
Mitochondrial RNA polymerase is an essential enzyme in erythrocytic stages of Plasmodium falciparum [0.03%]
恶性疟原虫红血球内期线粒体RNA聚合酶是一种重要的酶
Hangjun Ke,Joanne M Morrisey,Suresh M Ganesan et al.
Hangjun Ke et al.
We have shown that transgenic Plasmodium falciparum parasites expressing the yeast DHODH (dihydroorotate dehydrogenase) are independent of the mtETC (mitochondrial electron transport chain), suggesting that they might not need the mitochond...
Biochemical and molecular characterization of the pyrimidine biosynthetic enzyme dihydroorotate dehydrogenase from Toxoplasma gondii [0.03%]
弓形虫嘧啶生物合成酶二氢乳清酸脱氢酶的生化和分子研究
Miryam Andrea Hortua Triana,My-Hang Huynh,Manuel F Garavito et al.
Miryam Andrea Hortua Triana et al.
The pyrimidine biosynthesis pathway in the protozoan pathogen Toxoplasma gondii is essential for parasite growth during infection. To investigate the properties of dihydroorotate dehydrogenase (TgDHOD), the fourth enzyme in the T. gondii py...
Characterization of the M32 metallocarboxypeptidase of Trypanosoma brucei: differences and similarities with its orthologue in Trypanosoma cruzi [0.03%]
非洲锥虫布鲁西阶型的M32金属碳肽酶的特征：与克鲁兹锥虫正系同源物之间的差异和相似性
Alejandra P Frasch,Adriana K Carmona,Luiz Juliano et al.
Alejandra P Frasch et al.
Metallocarboxypeptidases (MCP) of the M32 family of peptidases have been identified in a number of prokaryotic organisms but they are absent from eukaryotic genomes with the remarkable exception of those of trypanosomatids. The genome of Tr...
Eva Bínová,Jarmila Klieščiková,David A Ashford et al.
Eva Bínová et al.
Genes for mannitol-metabolizing enzymes, mannitol phosphate dehydrogenase (MPDH) and mannitol dehydrogenase (MDH), have been recently identified in the genome of Acanthamoeba castellanii and their potential role in stress tolerance was prop...
Araceli Castillo-Romero,Barbara J Davids,Tineke Lauwaet et al.
Araceli Castillo-Romero et al.
The ability of Giardia to differentiate into cysts which survive in the environment and release the virulent trophozoites after ingestion in the small intestine is essential for transmission and disease. We examined the role of enolase, a g...
Leishmania (L.) amazonensis peptidase activities inside the living cells and in their lysates [0.03%]
利什曼原虫（亚马逊）肽酶活体内及体外的活性研究
Elide E Caroselli,Diego M Assis,Clara L Barbiéri et al.
Elide E Caroselli et al.
In this study we investigated the peptidase activity in Leishmania (L.) amazonensis live amastigote by confocal microscopy using peptidyl-MCA as substrates, the hydrolysis of which releases the MCA fluorophore inside the cells. Cell pre-tre...
Disparate phenotypic effects from the knockdown of various Trypanosoma brucei cytochrome c oxidase subunits [0.03%]
分别干扰不同血红素c氧化酶亚基可产生不同的表型效应
Anna Gnipová,Brian Panicucci,Zdeněk Paris et al.
Anna Gnipová et al.
The Trypanosoma brucei cytochrome c oxidase (respiratory complex IV) is a very divergent complex containing a surprisingly high number of trypanosomatid-specific subunits with unknown function. To gain insight into the functional organizati...
Transient and stable transfection in the protozoan parasite Entamoeba invadens [0.03%]
艾美球虫原生动物Entamoeba invadens的瞬时和稳定转染
Gretchen M Ehrenkaufer,Upinder Singh
Gretchen M Ehrenkaufer
Entamoeba histolytica is an important human pathogen and a major health problem worldwide. Many aspects of parasite biology can be studied with the exception of stage conversion, which cannot be reproduced adequately in E. histolytica. The ...