Manual curation of aquatic fungal ITS2 metabarcoding OTU matrices increases community identification on the genus and species level [0.03%]
手动整理水生真菌ITS2 metabarcoding OTU矩阵可增加属和种水平上的群落鉴定率
Christiane Baschien,Steffen Carl
Christiane Baschien
We conducted six metabarcoding studies from different aquatic habitats. It has been confirmed that fungal metabarcoding has the advantages of overcoming the cultivation bias, increasing the number of possible samples by high-throughput sequ...
A preliminary investigation on the influence of sample storage on the low-abundant ocular surface microbiome [0.03%]
样本储存对低丰度眼表微生物组影响的初步研究
Jérémy Rotzetter,Livia Spörri,Elio L Herzog et al.
Jérémy Rotzetter et al.
In this study, the impact of sample freezing prior to DNA extraction was investigated on a small sample set (n = 6) to determine whether this affects the composition of the ocular surface microbiome - a low biomass environment. Frozen sampl...
Exploring the role of phage resistance by using label-free SERS for biochemical profiling of gram-negative pathogen Pseudomonas aeruginosa strains with machine learning approach [0.03%]
基于无标记SERS的机器学习方法探索铜绿假单胞菌耐噬菌体机制及菌株快速筛查技术研究
Riha Zulfiqar,Asmat Ullah,Haq Nawaz et al.
Riha Zulfiqar et al.
Pseudomonas aeruginosa (P. aeruginosa) is an emerging gram-negative pathogen, accountable for diverse and chronic nosocomial infections, particularly in immunocompromised patients and frequently associated with multidrug resistant, posing a...
Utility of nucleic acid amplification tests in the diagnosis of osteoarticular tuberculosis [0.03%]
核酸扩增试验在骨关节结核诊断中应用价值的研究
Promod K Mehta,Khushpreet Kaur,Jefry Sebastian et al.
Promod K Mehta et al.
Osteoarticular TB (OATB) is a manifestation of extrapulmonary TB (EPTB), wherein spinal TB constitutes ~50% of OATB individuals. Diagnosis of OATB is quite difficult owing to deep inaccessible lesions within tissues, scanty bacterial load a...
Thermophilic bacteria mediated dye remediation in water and wastewater: Mechanistic and metabolic insights [0.03%]
水和废水介导的嗜热菌脱色修复：机制和代谢见解
Isha Dharsandia,Narendra Kumar,Ananya Tiwari et al.
Isha Dharsandia et al.
Industrial dye pollution is a major concern, especially in textiles, paper, and leather. Physical and chemical methods have high operational costs, often fail to degrade dyes completely, and may cause secondary pollution. Bioremediation wit...
Comparison of agar-based and checkerboard methods to assess phage-antimicrobial synergy in Pseudomonas aeruginosa [0.03%]
基于琼脂和棋盘法评估潘氏噬菌体与抗菌剂对绿脓杆菌协同作用的比较研究
Umut Yılmaz,Mehmet Ünlü,Gülhan Vardar Ünlü
Umut Yılmaz
Multidrug-resistant (MDR) Pseudomonas aeruginosa requires innovative therapeutic approaches. In this study, two lytic bacteriophages (BAUNPA1, BAUNPA3) were isolated from wastewater in Türkiye, and the combined efficacy of these phages wit...
Development and validation of TaqMan-based real-time PCR assays for translucent post larvae disease (TPD) detection in Penaeus vannamei [0.03%]
用于检测南美白对虾(Penaeus vannamei)透明亚oles病(Translucent Post Larvae Disease, TPD)的TaqMan实时荧光定量PCR方法的建立与验证
Hung N Mai,Nguyen Dinh-Hung,Arun K Dhar
Hung N Mai
Translucent Post larvae Disease (TPD), caused by Vibrio parahaemolyticus, has emerged as a critical disease impacting shrimp hatcheries across several Asian countries. This disease severely affects the early larval stages and can lead up to...
Immunomodulatory activity exploratory of riched gamma-aminobutyric acid gamma-aminobutyric acid in fermentated scallop skirt [0.03%]
富γ-氨基丁酸扇贝柱的免疫调节作用初探
Haoyue Yang,Ronge Xing,Kecheng Li et al.
Haoyue Yang et al.
Scallops belong to the Pectinidae family of the Mollusca phylum and are a major economic shellfish in ocean. With the rapid growth of shellfish aquaculture, the processing size of scallops has also increased significantly. During the proces...
Robust isolation of primary ovine monocytes for reservoir host cell-specific infection studies [0.03%]
绵羊单核细胞的主要分离以进行宿主细胞特异性感染研究
Benjamin U Bauer,Bidiepta Saha,Thierry Cottineau et al.
Benjamin U Bauer et al.
No widely available ovine monocyte/macrophage cell system exists. We present an affinity magnetic separation method combining gradient separation and magnetic bead selection to isolate pure primary ovine monocytes from blood, yielding large...
Design, expression, and immunogenicity evaluation of a novel multi-epitope chimeric protein (SOMP) against Salmonella Typhimurium [0.03%]
一种新型多表位嵌合蛋白(SOMP)的构建、表达及对伤寒沙门氏菌免疫原性的评价
Nima Rostami,Elahe Tajbakhsh,Shahram Nazarian et al.
Nima Rostami et al.
The emergence of multidrug-resistant Salmonella enterica serovar Typhimurium highlights an urgent need for effective vaccines. This study developed SOMP, a novel multi-epitope chimeric vaccine, by integrating conserved immunogenic domains f...