Quantification of anaerobic methanotrophic archaea Candidatus Methanoperedens of the ANME-2d cluster using new PCR primers [0.03%]
Rumito Maeda,Michiru Oda,Natsumi Inubashiri et al.
Rumito Maeda et al.
Archaea related to Candidatus Methanoperedens previously classified as anaerobic methanotrophs (ANME-2d), play critical roles in the anaerobic oxidation of methane in terrestrial environments. Despite their presumed widespread distribution,...
Ben Ahmed Anasse,Maryam Tidjani Alou,Camille Valles et al.
Ben Ahmed Anasse et al.
Culturomics, a high scale culture approach based on the multiplication of culture conditions, coupled with extended incubation times proved effective for discovering unknown microorganisms. While significant efforts were made to reduce the ...
An extraction-free HLPCR-Cas12a assay for ultra-sensitive and rapid detection of Acinetobacter baumannii and Klebsiella pneumoniae following craniotomy [0.03%]
Tao Fu,Fengmiao Wang,Jiangbin Ren et al.
Tao Fu et al.
Postoperative intracranial infection represents a significant complication in neurosurgical procedures, with Acinetobacter baumannii (Ab) and Klebsiella pneumoniae (Kp) frequently identified as common drug-resistant pathogens. In response t...
Preston Joshua Menezes
Preston Joshua Menezes
Limitations in rapid organism identification and access to appropriate cultivation media remain significant bottlenecks in microbiological research, particularly in laboratories without advanced diagnostic infrastructure. Many existing tool...
Microbial diversity of Nepalese fermented dish (Gundruk) and enhancing its fermentation using lactic acid Bacteria starter culture [0.03%]
Sadikshya Dahal,Saroj Poudel,Sarita Manandhar
Sadikshya Dahal
A typical non-salted fermented dish from Nepal, Gundruk is made from vegetables like radish, mustard, and rape in attempt to food preservation. The study primarily aimed to isolate lactic acid bacteria (LAB) from naturally fermented Gundruk...
Development and validation of a PCR-based detection assay for xerophilic Wallemia species informed by comparative mitogenomics [0.03%]
Shu Zhang,Yi-Lin An,Quan-Feng Cheng et al.
Shu Zhang et al.
Wallemia species, filamentous fungi known as foodborne pathogens, pose significant health risks to humans and animals. Despite their ecological significance, robust detection methodologies for these fungi remain underdeveloped. The polymorp...
Strain-specific challenges in applying CRISPR/Cas9-based genome editing in the novel genus Stutzerimonas [0.03%]
基于CRISPR/Cas9的基因组编辑在新型根际促生菌属Stutzerimonas中的应用挑战
Martin Menestreau,Åsa Frostegård,Morten Kjos
Martin Menestreau
Recently reclassified from the genus Pseudomonas, Stutzerimonas comprises metabolically versatile bacteria widely distributed across diverse environments and with a capacity to perform complete denitrification. Here, we evaluated the applic...
Performance evaluation of VITEK® MS PRIME compared to VITEK® MS [0.03%]
VITEK® MS PRIME与VITEK® MS性能评价比较研究
Sae Am Song
Sae Am Song
MALDI-TOF MS enables rapid microbial identification. We compared VITEK® MS and VITEK® MS PRIME using 363 clinical isolates. Identification rates were comparable (95.3% vs. 93.9%) with 98.3% concordance. PRIME reduced analysis time (23.8 v...
Comparative Study
Journal of microbiological methods. 2026 May:244:107479. DOI:10.1016/j.mimet.2026.107479 2026
Precision bacterial quantification via dual-enhanced U-net: Addressing division artifacts and spatial bias in microscopy analysis [0.03%]
基于双重增强U-Net的精密细菌定量方法及其在显微镜分析中分裂数目误差和空间偏差问题的解决方法
Ziyi Wang,Wei Xu,Qi Zhang et al.
Ziyi Wang et al.
Bacterial quantification is fundamental to biomedical research and clinical diagnostics. Traditional methods, such as plate counting and optical density (OD) measurement, are limited by poor timeliness and lack of scalability for high-throu...
Down-regulation of biofilm formation genes expression on some gram-negative bacteria by Fumaria officinalis extract [0.03%]
毛蕊花提取物下调部分革兰氏阴性菌生物被膜形成基因的表达
Ban O Abdulsattar,Jwan O Abdulsattar,Oday M Abdulmunem et al.
Ban O Abdulsattar et al.
The rapid rise of antibiotic-resistant Gram-negative pathogens underscores the critical need for alternative therapeutic approaches. Biofilm formation contributes significantly to pathogen resistance and persistence. This study aimed to inv...